Product Description for FGF basic / FGF2
Rabbit anti Human FGF basic / FGF2.
Presentation: Biotin
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.
Presentation: Biotin
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.
Properties for FGF basic / FGF2
| Product Category | Primary Antibodies |
|---|---|
| Target Category | |
| Quantity | 25 µg |
| Synonyms | BFGF, FGFB, Fibroblast growth factor 2 (basic), HBGF-2, HBGF2, Heparin-binding growth factor 2 |
| Presentation | Biotin |
| Reactivity | Hu |
| Applications | E, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Polyclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Add. information | Centrifuge vial prior to opening! |
| Application | Direct ELISA: To detect Human FGF-basic by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of ~1.0 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 2000-4000 pg/well of recombinant Human FGF-basic. Sandwich ELISA: To detect Human FGF-basic by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25–1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human FGF-basic (Cat.-No PP1007P) as a capture antibody, allows the detection of at least 2000–4000 pg/well of recombinant Human FGF-basic. Western Blot: To detect hFGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human FGF-basic is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
| Background | FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. |
| Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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| Gene ID | 2247 |
Accessory Products
Proteins and/or Positive Controls
Proteins for FGF basic / FGF2 (31 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP750002 |
FGF basic / FGF2 |
||||||
|
Human |
> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
0.1 mg /
$411.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP317426 |
FGF basic / FGF2 |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP720037 |
FGF basic / FGF2 |
||||||
|
Human |
> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
10 µg /
$299.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP723109 |
FGF basic / FGF2 |
||||||
|
Human |
> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli | ||||
| OriGene Technologies, Inc. | |||||||
| TP723110 |
FGF basic / FGF2 |
||||||
|
Human |
> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
$230.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP723719 |
FGF basic / FGF2 |
||||||
|
Human |
> 98 % Preparation: . Purity Detail: Purity is >98%, as determined by Coomassie stained SDS-PAGE. |
E. coli |
10 µg /
$135.00
|
|||
| OriGene Technologies, Inc. | |||||||
| AR09356PU-L |
FGF basic / FGF2 (recombinant) |
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|
Human | Purified | > 95 % Heparin affinity chromatography | E. coli | |||
| OriGene Technologies GmbH | |||||||
| AR09356PU-N |
FGF basic / FGF2 (recombinant) |
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|
|
Human | Purified | > 95 % Heparin affinity chromatography | E. coli | |||
| OriGene Technologies GmbH | |||||||
| AR09356PU-S |
FGF basic / FGF2 (recombinant) |
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|
|
Human | Purified | > 95 % Heparin affinity chromatography | E. coli | |||
| OriGene Technologies GmbH | |||||||
| AR10036PU-L |
FGF basic / FGF2 |
||||||
|
|
Human | Purified |
>98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Insect cells | |||
| OriGene Technologies GmbH | |||||||
| AR10036PU-N |
FGF basic / FGF2 |
||||||
|
|
Human | Purified |
>98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Insect cells | |||
| OriGene Technologies GmbH | |||||||
| AR10036PU-S |
FGF basic / FGF2 |
||||||
|
|
Human | Purified |
>98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Insect cells | |||
| OriGene Technologies GmbH | |||||||
| AR10039PU-L |
FGF basic / FGF2 (cell culture grade) |
||||||
|
|
Human | Purified |
> 98.0% as determined by both RP-HPLC and SDS-PAGE analysis. Purification Method: Proprietary chromatographic techniques. |
E. coli | |||
| OriGene Technologies GmbH | |||||||
| AR10039PU-N |
FGF basic / FGF2 (cell culture grade) |
||||||
|
|
Human | Purified |
> 98.0% as determined by both RP-HPLC and SDS-PAGE analysis. Purification Method: Proprietary chromatographic techniques. |
E. coli | |||
| OriGene Technologies GmbH | |||||||
| AR10039PU-S |
FGF basic / FGF2 (cell culture grade) |
||||||
|
|
Human | Purified |
> 98.0% as determined by both RP-HPLC and SDS-PAGE analysis. Purification Method: Proprietary chromatographic techniques. |
E. coli | |||
| OriGene Technologies GmbH | |||||||
| AR10041PU-L |
FGF basic / FGF2 (Animal Product Free Grade) |
||||||
|
|
Human | Purified |
> 95% pure as determined by 10% PAGE (coomassie staining). Purification Method: DEAE sepharose, Affinity Chromatography. |
E. coli | |||
| OriGene Technologies GmbH | |||||||
| AR10042PU-L |
FGF basic / FGF2 (Stem Cell Grade) |
||||||
|
|
Human | Purified |
> 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
E. coli | |||
| OriGene Technologies GmbH | |||||||
| AR10042PU-N |
FGF basic / FGF2 (Stem Cell Grade) |
||||||
|
|
Human | Purified |
> 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
E. coli | |||
| OriGene Technologies GmbH | |||||||
| AR10042PU-S |
FGF basic / FGF2 (Stem Cell Grade) |
||||||
|
|
Human | Purified |
> 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
E. coli | |||
| OriGene Technologies GmbH | |||||||
| AR31105PU-N |
FGF basic / FGF2 (164 amino acids) |
||||||
|
|
Human | Purified | > 98 % pure by SDS-PAGE and HPLC analyses | E. coli | |||
| OriGene Technologies GmbH | |||||||
| AR31105PU-S |
FGF basic / FGF2 (146 amino acids) |
||||||
|
|
Human | Purified | > 98 % pure by SDS-PAGE and HPLC analyses | E. coli | |||
| OriGene Technologies GmbH | |||||||
| AR50023PU-N |
FGF basic / FGF2 (143-288) (recombinant) |
||||||
|
Human | Purified | > 95 % by SDS - PAGE | E. coli | |||
| OriGene Technologies GmbH | |||||||
| AR50023PU-S |
FGF basic / FGF2 (143-288) (recombinant) |
||||||
|
|
Human | Purified | > 95 % by SDS - PAGE | E. coli | |||
| OriGene Technologies GmbH | |||||||
| DA3503 |
FGF basic / FGF2 |
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|
Human | Purified | > 98 % by SDS-PAGE and visualised by silver stain | E. coli |
10 µg /
$160.00
|
||
| OriGene Technologies GmbH | |||||||
| DA3503X |
FGF basic / FGF2 |
||||||
|
|
Human | Purified | > 98 % by SDS-PAGE and visualised by silver stain | E. coli |
25 µg /
$180.00
|
||
| OriGene Technologies GmbH | |||||||
| DA3503XC |
FGF basic / FGF2 |
||||||
|
|
Human | Purified | > 98 % by SDS-PAGE and visualised by silver stain | E. coli |
50 µg /
$240.00
|
||
| OriGene Technologies GmbH | |||||||
| PA055 |
FGF basic / FGF2 (154 amino acids) |
||||||
|
|
Human | Purified | > 95 % pure by SDS-PAGE and HPLC analyses | E. coli | |||
| OriGene Technologies GmbH | |||||||
| PA055X |
FGF basic / FGF2 (154 amino acids) |
||||||
|
|
Human | Purified | > 95 % pure by SDS-PAGE and HPLC analyses | E. coli | |||
| OriGene Technologies GmbH | |||||||
| PA1015 |
FGF basic / FGF2 |
||||||
|
|
Human | Purified |
> 95 % Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Insect cells | |||
| OriGene Technologies GmbH | |||||||
| PA1015X |
FGF basic / FGF2 |
||||||
|
|
Human | Purified |
> 95 % Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Insect cells | |||
| OriGene Technologies GmbH | |||||||
| PA1015XC |
FGF basic / FGF2 |
||||||
|
|
Human | Purified |
> 95 % Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Insect cells | |||
| OriGene Technologies GmbH | |||||||
Recommended Secondary Antibodies for FGF basic / FGF2 (9 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | AP | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | Biotin | Rb | E, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | Rb | E, ICC/IF, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | Rb | E, P, WB | |||
| OriGene Technologies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | Rb | E, F, ICC/IF | |||
| OriGene Technologies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
|
Donkey | PE | Rb | F | |||
| OriGene Technologies GmbH | |||||||
| R1458C2 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C3 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1458C5 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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