discontinued

PA1015X FGF basic / FGF2

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FGF basic / FGF2

PA1015X

Product Description for FGF basic / FGF2

FGF basic / FGF2.
Presentation: Purified

Properties for FGF basic / FGF2

Product Category Proteins & Growth Factors
Target Category
Quantity 10 µg
Synonyms BFGF, FGFB, Fibroblast growth factor 2 (basic), HBGF-2, HBGF2, Heparin-binding growth factor 2
Presentation Purified
Molecular weight 17 kDa
Source Insect cells
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P09038
Purity > 95 % Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Add. information Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Fibroblast Growth Factor-b as a Reference Standard.
Background Basic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
Concentration 0.5mg/ml
Storage Store the protein undiluted at 2-8°C for 3 weeks or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Shelf life: one year from despatch.
Source Baculovirus
Description
Description:
Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in Sf9 insect cells is a single, glycosylated, polypeptide chain containing 155 amino acids. The FGF-basic is purified by proprietary chromatographic techniques.
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Ala-Gly-Ser-Ile.
Biological activity:
The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5 ng/ml.
Molecular weight:
17353 Dalton
Specific activity:
2 x 10e6 Units/mg
Format
Buffer System:
20mM Tris pH=7.9, 100mM KCl, 1mM DTT and 20% glycerol
Purity:
>95% Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
State:
Liquid purified protein (sterile filtered)
Purified
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