AR10036PU-L FGF basic / FGF2

Swiss Prot Num:

P09038

Basic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.

Description:

Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in Insect Cell is a single, glycosylated, polypeptide chain containing 155 amino acids and having a Molecular Mass of 17353 Dalton.
The FGF-basic is purified by proprietary chromatographic techniques.

AA Sequence:

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Ala-Gly-Ser-Ile.

Biological activity:

The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by ³H-thymidine uptake) is 0.5 ng/ml, corresponding to a specific activity of 2 x 10⁶ Units/mg.

Buffer System:

20mM Tris pH 7.9, 100mM KCl, 1mM DTT and 20% Glycerol.

Purity:

>98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

State:

Liquid sterile filtered solution
Purified