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AR09356PU-N FGF basic / FGF2 (recombinant)

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FGF basic / FGF2

AR09356PU-N

Product Description for FGF basic / FGF2

FGF basic / FGF2.
Presentation: Purified

Properties for FGF basic / FGF2

Product Category Proteins & Growth Factors
Target Category
Quantity 50 µg
Synonyms BFGF, FGFB, Fibroblast growth factor 2 (basic), HBGF-2, HBGF2, Heparin-binding growth factor 2
Presentation Purified
Molecular weight 18
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P09038
Purity > 95 % Heparin affinity chromatography
Application Characterized growth factor additive in cell culture media for mesoderm and neuroectoderm-derived cells.
Biological Test System
- Cell proliferation assay with BAE cells
- Rabbit cornea in vivo assay
Background FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
General Readings
  1. Abraham JA, Whang JL, Tumolo A, Mergia A, Friedman J, Gospodarowicz D, et al. Human basic fibroblast growth factor: nucleotide sequence and genomic organization. EMBO J. 1986 Oct;5(10):2523-8. PubMed PMID: 3780670. (Free PMC Article available)
  2. Gospodarowicz D, Neufeld G, Schweigerer L. Molecular and biological characterization of fibroblast growth factor, an angiogenic factor which also controls the proliferation and differentiation of mesoderm and neuroectoderm derived cells. Cell Differ. 1986 Jul;19(1):1-17. PubMed PMID: 2425984.
  3. Lobb R, Sasse J, Sullivan R, Shing Y, D'Amore P, Jacobs J, et al. Purification and characterization of heparin-binding endothelial cell growth factors. J Biol Chem. 1986 Feb 5;261(4):1924-8. PubMed PMID: 3003088.
  4. Iwane M, Kurokawa T, Sasada R, Seno M, Nakagawa S, Igarashi K. Expression of cDNA encoding human basic fibroblast growth factor in E. coli. Biochem Biophys Res Commun. 1987 Jul 31;146(2):470-7. PubMed PMID: 3304281.
  5. Knoerzer W, Binder HP, Schneider K, Gruss P, McCarthy JE, Risau W. Expression of synthetic genes encoding bovine and human basic fibroblast growth factors (bFGFs) in Escherichia coli. Gene. 1989 Jan 30;75(1):21-30. PubMed PMID: 2470650.
  6. Boehnke K, Mirancea N, Pavesio A, Fusenig NE, Boukamp P, Stark HJ. Effects of fibroblasts and microenvironment on epidermal regeneration and tissue function in long-term skin equivalents. Eur J Cell Biol. 2007 Dec;86(11-12):731-46. Epub 2007 Feb 9. PubMed PMID: 17292509.
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the antibody at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human basic Fibroblast Growth Factor (hbFGF).
Biological activity:
< 1 ng/ml for half maximum stimulation of cell proliferation with bovine aortic endothelial cells (BAE cells).
Format
Buffer System:
100 µl (10 µg; 50 µg) or 1 ml (1 mg) phosphate buffered saline (pH 6), supplemented with 5 mM glutathion
Purity:
>95% Heparin affinity chromatography
State:
Lyophilised purified protein
Purified
Reconstitution:
Reconstitute in 100 µl (10 µg; 50 µg) or 1 ml (1 mg) dist. H2O, dilute further in PBS as required.
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