AR10039PU-N FGF basic / FGF2 (cell culture grade)

Swiss Prot Num:

P09038

Prior to reconstitution store at 2-8°Cfor one month or desiccated below -18°C for longer.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Description:

Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids. 
Protein Content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein. 
2. Analysis by RP-HPLC, using a standard solution of FGF-2 as a Reference Standard.

AA Sequence:

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Glu-Gly-Glu-Ile

Biological activity:

The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is < 0.5 ng/ml, corresponding to a specific activity of 2 x10⁶ Units/mg.

Buffer System:

Lyophilized from a concentrated (1.0 mg/ml) sterile solution containing 5mM NaPO₄ and 100mM NaCl

Purity:

> 98.0% as determined by both  RP-HPLC and SDS-PAGE analysis. 
Purification Method: Proprietary chromatographic techniques.

State:

Sterile Filtered White lyophilized (freeze-dried) powder
Purified

Reconstitution:

Restore in sterile 18MΩ-cm^-1 H₂O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions