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FGF basic / FGF2 antibody

Principal name

FGF basic / FGF2 antibody

Alternative names for FGF basic / FGF2 antibody

FGFB, Heparin-binding growth factor 2, Fibroblast growth factor 2 (basic), BFGF, HBGF-2, HBGF2

SwissProt ID

P03969 (Bovin), P09038 (Human), P12226 (Xenla), P13109 (Rat), P15655 (Mouse), P20003 (Sheep), P48799 (Rabit), P48800 (Chick), Q60487 (Cavpo)

Gene ID

2247 (FGF2), 281161, 14173 (Fgf2), 54250

Ncbi ID

NP_001997, NP_001997.5, 9606, NP_776481, NP_032032, NP_062178, NP_062178.1, NP_032032.1, AAI66646.1, NM_002006, NM_008006, NM_019305

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), Rb (Rabbit), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Sh (Sheep)

Available hosts

Mouse, Rabbit, Chicken, Sheep

Available applications

Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Frozen Sections (C), Radioimmunoassay (R), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Neutralisation (NEUT), Flow Cytometry (F)

Background of FGF basic / FGF2 antibody

The FGF family is composed of at least 22 polypeptides that show a variety of biological activities towards cells of mesenchymal, neuronal and epithelial origin. All members are heparin-binding growth factors (HBGF). Until the structure of basic fibroblast growth factor (bFGF/FGF-2) was determined, a number of synonyms was used to describe this growth factor. As is often the case, the nomenclature reflected the observed activities reported by individual groups. Basic FGF has been reported as leukemia growth factor, macrophage growth factor, endothelial growth factor and tumor angiogenesis factor. The eventual isolation and characterization of bFGF was done from soluble brain extracts. bFGF was found to have a molecular mass of 16 kDa and to be 154 amino acids in length. Interestingly, bFGF contains no hydrophobic leader sequence previously thougth to be required for cell secretion. Basic FGF bears 55% homology to acidic FGF and also seems to exist in three forms: the 154 amino-acid form and two other truncated versions of 146 and 131 amino acids lacking the N-terminal 9 and 24 residues. Acidic and basic FGF compete for the binding to 125 kDa and 145 kDa receptor species. However, acidic FGF has a higher affinity for the 125 kDa species, while basic FGF has a higher affinity for the 145 kDa species. FGF receptor activation leads to the activation of MAP kinase and protein kinase C. FGF's induce the proliferative response in cells derived from mesoderm and neuroectoderm. It seems that basic FGF reduces the average doubling time by shortening the G1 phase of the cell cycle. Furthermore, it has been reported to induce the release of plasminogen activator by endothelial cells. Perhaps one of the most potentially significant applications of bFGF is related to its reported ability to induce angiogenesis.

General readings

Nakayama T., et al. (2007) Blood. 109(2):1363-72.A E Eriksson., et al. (1991) PNAS. 88: 3441-3445

PM1203P

Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 0.25 µg/ml-0.50 µg/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Tissue samples were provided by the Cooperative Human Tissue Network which is funded by the National Cancer Institute.

PM1203P

Immunohistochemistry: Antibody Cat.no. PP1007P stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. Tissue samples were provided by the Cooperative Human Tissue Network which is funded by the National Cancer Institute.

SDS-PAGE analysis of recombinant mouse FGF-2 (basic). Samples were loaded in 15% SDS-polyacrylamide gel under reducing and nonreducing condition and stained with Coomassie blue.

FGF2-induced proliferation of NHDF cells. NHDF cells were stimulated with increasing amounts of human basic FGF.

FGF2-induced proliferation of HUVE cells. HUVECs were stimulated with increasing amounts of human basic FGF.

Test of cross-reactivity of anti-Human FGF-2 polyclonal antibody by Western blot analysis. Recombinant human, mouse and Rat FGF-2 produced in E. coli were loaded under reducing conditions in 15% SDS-polyacrylamide gel. Anti-human FGF-2 recognizes also the mouse and the rat form.

Western analysis of recombinant human (DA3503XC), mouse (AR31056PU-L) and rat (AR31055PU-L) FGF-2 (basic) using a polyclonal antibody directed against recombinant human FGF-2. As expected the antibody cross reacts with the mouse and rat proteins.

FGF-2 Sandwich-ELISA using a polyclonal Goat anti-human FGF-2 as capture antibody and recombinant human FGF-2 (Cat.-No DA3503X) as standard. The polyclonal rabbit anti-human FGF-2 antibody (Cat.-No DP3506)in combination with a goat anti-rabbit-Biotin was used for detection.

Immunohistochemistry of FGF-2 staining in Paraffin sections of Bovine follicule tissue. Endogenous peroxidase activity was blocked by incubating the section in 1% H2O2 in Methanol for 30 minutes. The section was incubated with AP02009SU (1/1200) and detected using Biotinylated secondary antibody with ABC Kit. DAB was used as the Chromogen. (A) AP02009SU stains the larger vessels in the theca externa, but not lymphatic vessels (arrow). Scale bar = 100 µm. Berisha B et al (2000) J Endocrinol167(3):371-82.

Western blot analysis with 50 ng recombinant human FGF2.

PAB8713

Western blot analysis of Human bFGF using Monoclonal anti-Human bFGF antibody

AM09155PU-N

Western blot analysis of Human bFGF using Monoclonal anti-Human bFGF antibody

AM09157PU-N

Western blot analysis of Human bFGF using Monoclonal anti-Human bFGF antibody

AM09159PU-N

Western blot analysis of Human bFGF using Monoclonal anti-Human bFGF antibody

AM09160PU-N

Human A375; . Sample Type :. Human A375 cells. Primary Antibody Dilution :. 1:100. Secondary Antibody :. Anti-rabbit-Alexa-546. Secondary Antibody Dilution :. 1:100. Color/Signal Descriptions :. Red: FGF2 Blue: Nuclei. Gene Name :. FGF2. Submitted by :. Igor Prudovsky, PhD, DSc, Principal Investigator, Center for Molecular Medicine, Maine Medical Center Research Institute, 81 Research Dr. Scarborough, ME 4074; FGF2 antibody - middle region (ARP42005_P050) in Human A375 cells using Immunohistochemistry

ARP42005_P050

Human Jurkat; WB Suggested Anti-FGF2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; FGF2 antibody - middle region (ARP42005_P050) in Human Jurkat cells using Western Blot

ARP42005_P050

Immunohistochemical analysis of Paraffin Embeedded Mammary cancer sections using FGF2 antibody. Staining FGF2 in Cytoplasm.DAB chromogenic reaction.

AP23263PU-N

Western blot of Rat brain tissue lysis using FGF2 antibody

AP23263PU-N

FGF2 Antibody (Center) (Cat. #AP51656PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FGF2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

AP51656PU-N

FGF2 Antibody (Center) (Cat. #AP51656PU-N) western blot analysis in NCI-H460cell line lysates (35µg/lane).This demonstrates the FGF2 antibody detected the FGF2 protein (arrow).

AP51656PU-N

Dose-dependent stimulation of cell proliferation in HUVE cells by recombinant rat and mouse FGF-2 (basic). Values are the means (±SD) of triplicate determinations and expressed as percentage of control.

SDS-PAGE analysis of recombinant Rat FGF-2 (basic): Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Coomassie blue.

Dose-dependent stimulation of cell proliferation in HUVE cells by recombinant Rat and Mouse FGF-2 (basic). Values are the means (±SD) of triplicate determinations and expressed as percentage of control.

SDS-PAGE (15%) with subsequent Silver staining of recombinant Mouse FGF-2 (basic). Lane 1: MW marker. Lane 2: Mouse FGF-2.

Immunohistochemical analysis of paraffin-embedded human recturn adenocarcinoma tissue showing cytoplasmic localization using FGF2 mouse mAb with DAB staining.

AM06116SU-N

Western blot analysis using FGF2 mouse mAb against truncated FGF2 recombinant protein.

AM06116SU-N

ISOKineTM bFGF vs our potent and proven e-coli derived bFGF

PR80001-10

ISOKine^TM bFGF vs our potent and proven e-coli derived bFGF

PR80001-50

ISOKine^TM bFGF vs our potent and proven e-coli derived bFGF.

PR80001-100

Human FGF2 (Fibroblast Growth Factor 2) recombinant protein

Human FGF2 (Fibroblast Growth Factor 2) 18kDa isoform recombinant protein

Representative Standard Curve

CEK1151

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BFGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500012)

TA500012

TA500012

Anti-BFGF mouse monoclonal antibody (TA500012) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BFGF(RC217426).

TA500012

TA500012

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFGF (RC217426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFGF.

TA500012

Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-BFGF monoclonal antibody at 1:200 dilution.

TA500012

TA500013

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BFGF monoclonal antibody.

TA500013

BFGF antibody (2H11) at 1:10000 + Recombinant human bFGF (Cat# RC217426)

TA500013

TA500013

TA500269

HEK293T cells transfected with either RC217426 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BFGF antibody(TA500269), and then analyzed by flow cytometry.

TA500269

TP317426

TP720035

TP720037

TP723107

TP723108

TP723109

TP723110

TP723719

TP750002

Western blot analysis using recombinant rat FGF-2. Lane 1: 10 ng, Lane 2: 50 ng, Lane 3: 100 ng, Lane 4: 250 ng.

TA319021

Predicted cell location: Cytoplasm . Positive control: Human breast cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using FGF2 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)

TA321420

Predicted band size: 31kDa. Positive control: Mouse lung tissue lysate . Recommended dilution: 1/1000-5000

TA321421

Predicted cell location: Cytoplasm . Positive control: Human breast cancer tissue. Recommended dilution: 1/100-500 The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using FGF2 antibody at dilution 1/100, on the right is treated with the synthetic peptide. (Original magnification: x200)

TA321421

To detect hFGF-basic by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hFGF-basic.

TA328310

To detect hFGF-basic by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human FGF-basic (TA328309) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hFGF-basic.

TA328310

To detect Human FGF-basic by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human FGF-basic (TA328310) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human FGF-basic.

TA328311

TA328311

TA328311

WB Suggested Anti-FGF2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate

TA346315

Sample Type: Human A375 cells Primary Antibody Dilution: 1: 100; Secondary Antibody: Anti-rabbit-Alexa-546; Secondary Antibody: ilution: 1: 100; Color/Signal Descriptions: Red: FGF2 Blue: Nuclei; Gene Name: FGF2;

TA346315

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
DP3506	FGF basic / FGF2 antibody
	Rabbit			Azide Free	Hu	FN, WB	0.2 mg / $290.00
							OriGene Technologies GmbH
+2 additional images
DP3506S	FGF basic / FGF2 antibody
	Rabbit			Azide Free	Hu	FN, WB	0.1 mg / $230.00
							OriGene Technologies GmbH
+2 additional images
PM1203P	FGF basic / FGF2 antibody
	Mouse	IgG1		Azide Free	Hu	E, P, WB	0.5 mg / $220.00
							OriGene Technologies GmbH
+1 additional image
PP1007B1	FGF basic / FGF2 antibody
	Rabbit			Biotin	Hu	E, WB	25 µg / $250.00
							OriGene Technologies GmbH
+1 additional image
PP1007B2	FGF basic / FGF2 antibody
	Rabbit			Biotin	Hu	E, WB	50 µg / $340.00
							OriGene Technologies GmbH
+1 additional image
PP1007P1	FGF basic / FGF2 antibody
	Rabbit			Aff - Purified	Hu	E, FN, P, WB	50 µg / $250.00
							OriGene Technologies GmbH
+1 additional image
PP1007P2	FGF basic / FGF2 antibody
	Rabbit			Aff - Purified	Hu	E, FN, P, WB	0.1 mg / $340.00
							OriGene Technologies GmbH
+1 additional image
SM1286P	FGF basic / FGF2 antibody
	Mouse	IgG2a	MC-GF1	Purified	Hu	C, E, P, WB	0.5 mg / $560.00
							OriGene Technologies GmbH

CRISPR

Catalog No.	Species	Donor Vector
KN217426	FGF2 - human gene knockout kit via CRISPR
	Human	GFP-Puro	1 kit / $1,290.00
			OriGene Technologies, Inc.
KN217426BN	FGF2 - human gene knockout kit via CRISPR
	Human	mBFP-Neo	1 kit / $1,290.00
			OriGene Technologies, Inc.
KN217426LP	FGF2 - human gene knockout kit via CRISPR
	Human	Luciferase-Puro	1 kit / $1,290.00
			OriGene Technologies, Inc.
KN217426RB	FGF2 - human gene knockout kit via CRISPR
	Human	RFP-BSD	1 kit / $1,290.00
			OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP750002	FGF basic / FGF2
	Recombinant protein of human Fibroblast Growth Factor-basic (FGF2) produced in E. coli
	Human	> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	0.1 mg / $411.00
				OriGene Technologies, Inc.
	Published recently!
TP317426	FGF basic / FGF2
	Recombinant protein of human fibroblast growth factor 2 (basic) (FGF2)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / $748.00
				OriGene Technologies, Inc.
TP720035	FGF basic / FGF2
	Recombinant protein of mouse fibroblast growth factor basic
	Mouse	> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	10 µg / $299.00
				OriGene Technologies, Inc.
TP720037	FGF basic / FGF2
	Recombinant protein of human fibroblast growth factor 2 (basic) (FGF2)
	Human	> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	10 µg / $299.00
				OriGene Technologies, Inc.
TP723107	FGF basic / FGF2
	Purified recombinant protein of Mouse fibroblast growth factor 2 (Fgf2).
	Mouse	> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	50 µg / $230.00
				OriGene Technologies, Inc.

TP723108	FGF basic / FGF2
	Purified recombinant protein of Rat fibroblast growth factor 2 (Fgf2).
	Rat	> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	50 µg / $230.00
				OriGene Technologies, Inc.

TP723110	FGF basic / FGF2
	Purified recombinant protein of Human fibroblast growth factor 2 (basic) (FGF2).
	Human	> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	50 µg / $230.00
				OriGene Technologies, Inc.

TP723719	FGF basic / FGF2
	Purified recombinant protein of Human fibroblast growth factor 2 (basic) (FGF2)
	Human	> 98 % Preparation: . Purity Detail: Purity is >98%, as determined by Coomassie stained SDS-PAGE.	E. coli	10 µg / $135.00
				OriGene Technologies, Inc.

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FGF basic / FGF2 antibody

Principal name

Alternative names for FGF basic / FGF2 antibody

SwissProt ID

Gene ID

Ncbi ID

Available reactivities

Available hosts

Available applications

Background of FGF basic / FGF2 antibody

General readings

Primary Antibodies