TYRO3 antibody
Principal name
TYRO3 antibody
Alternative names for TYRO3 antibody
BYK, DTK, RSE, SKY, Tyrosine-protein kinase receptor TYRO3
SwissProt ID
P55144 (Mouse), P55146 (Rat), Q06418 (Human), Q8QFP9 (Xenla), Q98949 (Chick), Q9YI66 (Danre)
Ncbi ID
9606, NP_006284, NP_006284.2, NM_006293.2, NM_006293, 10090, NM_019392, NM_017092, BC082325
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F), Luminex (LMNX)
Background of TYRO3 antibody
The transmembrane signalling by receptor tyrosine kinases invloves the activation of cell surface receptors as a result of ligand binding and subsequenct actviation of cytoplasmic tyrosine kinase domain that resides in the cytoplasmic loop. The tryrosine kinase receptors are critical for cell survival, proliferation and cell differentiation. The Tyro 3 family receptor members include Tyro 3 (also known as Sky, Brt, Tif, Rse, Dtk or Etk); Axl ½ (also known as Ark, Ufo, Tyro 7) and Mer (also known as Eyk, Nyk, tyro 12) (1-2). These receptors share distinctive structural domains in the extracellular region, composed of 2 immunoglobulin-related domains linked to 2 fibronectin type III repeats. These structural motifs are characteristics of cell adhesion molecules such as neuronal cell adhesion molecule (involved in proper migration of neuronal crest cells), and protein phosphatase-kappa and m.
The growth-arrest specific gene 6 (Gas 6), initially cloned form serum starved 3T3 fibroblast, causes cell cycle re-entry of serum starved 3T3 cells and also prevent them from apoptotic cell death caused by complete serum removal. The Gas 6 has been identified as a common ligand for all Tyro3 family receptor kinases including Mertk, tyro3 and Axl1/2. Triple mutant mouse (Tyro3-/-; Axl-/- ; Mer-/-) exhibit multiple major organ defects suggesting their involvement in differentiation and growth. Tyro3 receptors are widely expressed in neuronal tissue, lymphoid, vascular, reproductive and retinal tissues (3). The role of Tyro3 receptors in spermatogenesis is just emerging (4). Tyro3 is a 880-890 aa protein with multiple functional domains characteristics of receptor tyrosine kinases.
Western Blot analysis of TYRO3 expression in transfected 293T cell line by TYRO3 monoclonal antibody (M05), clone 4F6.Lane 1: TYRO3 transfected lysate(96.9 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of TYRO3 over-expressed 293 cell line, cotransfected with TYRO3 Validated Chimera RNAi ( Cat # H00007301-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TYRO3 monoclonal antibody (M05) clone 4F6 (Cat # H00007301-M05 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Detection limit for recombinant GST tagged TYRO3 is approximately 0.1ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with TYRO3 polyclonal antibody ( Cat # PAB3391 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Western blot analysis of TYRO3 polyclonal antibody ( Cat # PAB3391 ) in mouse brain lysate. SKY ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
TYRO3 MaxPab rabbit polyclonal antibody. Western Blot analysis of TYRO3 expression in mouse lung.
Western Blot analysis of TYRO3 expression in transfected 293T cell line (H00007301-T01) by TYRO3 MaxPab polyclonal antibody.Lane 1: TYRO3 transfected lysate(96.90 KDa).Lane 2: Non-transfected lysate.
Figure 2. Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with TYRO3 Antibody (Cat.#AM11072PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Figure 1. Western blot analysis of anti-TYRO3 Monoclonal Antibody (Cat.#AM11072PU-N) by TYRO3 recombinant protein. TYRO3 protein (arrow) was detected using the purified Mab.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
(LEFT)Western blot analysis of anti-SKY Pab in mouse brain lysate. SKY (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. (RIGHT)Western blot analysis of SKY (arrow) using rabbit polyclonal SKY Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SKY gene (Lane 2).
Western blot of TYRO3 antibody (AP10364PU-N at 1/750 dilution)
Immunofluorescence staining of methanol-fixed MCF-7 and HepG2 cells showing membrane and cytoplasmic localization.
Western blot analysis using TYRO3 antibody Cat.-No AM06173SU-N against truncated TYRO3 recombinant protein.
Western blot analysis using TYRO3 antibody Cat.-No AM06327SU-N against extracellular domain of Human TYRO3 (aa41-429).
Immunofluorescence analysis of Hela (Left) and MCF-7 (Right) cells using Tyro3 antibody Cat.-NO AM06410SU-N (green).
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500413)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500413)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500413)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500413)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500413)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500413)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500413)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500413)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500413)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500413)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500413)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500413)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500413)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYRO3 (RC208260, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3.
Immunoprecipitation(IP) of TYRO3 by using TrueMab monoclonal anti-TYRO3 antibodies (Negative control: IP without adding anti-TYRO3 antibody.). For each experiment, 500ul of DDK tagged TYRO3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-TYRO3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500414)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500414)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500414)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500414)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500414)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500414)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500414)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500414)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500414)
Anti-TYRO3 mouse monoclonal antibody (TA500414) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TYRO3(RC208260).
Immunoprecipitation(IP) of TYRO3 by using TrueMab monoclonal anti-TYRO3 antibodies (Negative control: IP without adding anti-TYRO3 antibody.). For each experiment, 500ul of DDK tagged TYRO3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-TYRO3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYRO3 (RC208260, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3.
Anti-TYRO3 mouse monoclonal antibody (TA500415) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TYRO3(RC208260).
Immunoprecipitation(IP) of TYRO3 by using TrueMab monoclonal anti-TYRO3 antibodies (Negative control: IP without adding anti-TYRO3 antibody.). For each experiment, 500ul of DDK tagged TYRO3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-TYRO3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC208260 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TYRO3 antibody(TA500415), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500416)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500416)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500416)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500416)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500416)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500416)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500416)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500416)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYRO3 (RC208260, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3.
Immunoprecipitation(IP) of TYRO3 by using TrueMab monoclonal anti-TYRO3 antibodies (Negative control: IP without adding anti-TYRO3 antibody.). For each experiment, 500ul of DDK tagged TYRO3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-TYRO3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500417)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500417)
Anti-TYRO3 mouse monoclonal antibody (TA500417) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TYRO3(RC208260).
Immunoprecipitation(IP) of TYRO3 by using TrueMab monoclonal anti-TYRO3 antibodies (Negative control: IP without adding anti-TYRO3 antibody.). For each experiment, 500ul of DDK tagged TYRO3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-TYRO3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYRO3 (RC208260, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500420)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500420)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500420)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500420)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500420)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500420)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500420)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500420)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500420)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500420)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500420)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500420)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYRO3 (RC208260, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3.
Immunoprecipitation(IP) of TYRO3 by using TrueMab monoclonal anti-TYRO3 antibodies (Negative control: IP without adding anti-TYRO3 antibody.). For each experiment, 500ul of DDK tagged TYRO3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-TYRO3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500421)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500421)
Anti-TYRO3 mouse monoclonal antibody (TA500421) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TYRO3(RC208260).
Immunoprecipitation(IP) of TYRO3 by using TrueMab monoclonal anti-TYRO3 antibodies (Negative control: IP without adding anti-TYRO3 antibody.). For each experiment, 500ul of DDK tagged TYRO3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-TYRO3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYRO3 (RC208260, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3.
HEK293T cells transfected with either RC208260 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TYRO3 antibody(TA500421), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYRO3 (RC208260, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TYRO3 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
ELISA: TYRO3 Antibody
TYRO3 Luminex Elisa with 5B4 Capture (TA600225) and 9B12 Detection (TA700225) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 4C8 Capture (TA600226) and 9B12 Detection (TA700225) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 2D11 Capture (TA600227) and 5B4 Detection (TA700226) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 2D11 Capture (TA600227) and 9B12 Detection (TA700225) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 ELISA with 2G2 Capture (TA600228) and 5B4 Detection (TA700226) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 ELISA with 2G2 Capture (TA600228) and 9B12 Detection (TA700225) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 2G2 Capture (TA600228) and 4C8 Detection (TA700228) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 2G2 Capture (TA600228) and 5B4 Detection (TA700226) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 2G2 Capture (TA600228) and 2D11 Detection (TA700229) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 2G2 Capture (TA600228) and 9B12 Detection (TA700225) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 2G2 Capture (TA600228) and 5B7 Detection (TA700227) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 ELISA with 9B12 Capture (TA600229) and 2D11 Detection (TA700229) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 ELISA with 9B12 Capture (TA600229) and 5B4 Detection (TA700226) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 9B12 Capture (TA600229) and 5B4 Detection (TA700226) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 9B12 Capture (TA600229) and 5B7 Detection (TA700227) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 9B12 Capture (TA600229) and 2D11 Detection (TA700229) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 9B12 Capture (TA600229) and 4C8 Detection (TA700228) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 ELISA with 2G2 Capture (TA600228) and 9B12 Detection (TA700225) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 5B4 Capture (TA600225) and 9B12 Detection (TA700225) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 4C8 Capture (TA600226) and 9B12 Detection (TA700225) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 2D11 Capture (TA600227) and 9B12 Detection (TA700225) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 2G2 Capture (TA600228) and 9B12 Detection (TA700225) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 ELISA with 9B12 Capture (TA600229) and 5B4 Detection (TA700226) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 ELISA with 2G2 Capture (TA600228) and 5B4 Detection (TA700226) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 2D11 Capture (TA600227) and 5B4 Detection (TA700226) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 9B12 Capture (TA600229) and 5B4 Detection (TA700226) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 2G2 Capture (TA600228) and 5B4 Detection (TA700226) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 2G2 Capture (TA600228) and 5B7 Detection (TA700227) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 9B12 Capture (TA600229) and 5B7 Detection (TA700227) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 2G2 Capture (TA600228) and 4C8 Detection (TA700228) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 9B12 Capture (TA600229) and 4C8 Detection (TA700228) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 ELISA with 9B12 Capture (TA600229) and 2D11 Detection (TA700229) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 2G2 Capture (TA600228) and 2D11 Detection (TA700229) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
TYRO3 Luminex Elisa with 9B12 Capture (TA600229) and 2D11 Detection (TA700229) Antibodies. Substrate used: Recombinant Human TYRO3 (TP308260)
Western blot analysis of lysate from U-87 MG cell line, using TYRO3 Antibody(Cat. #TA302134). TA302134 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
Formalin-fixed and paraffin-embedded human brain tissue reacted with TYRO3 antibody (Cat.#TA302134), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western Blot: TYRO3 Antibody [TA336749] - Lane 1: Western blot on normal human brain, Lane 2: Western blot on normal mouse brain.
Immunocytochemistry/Immunofluorescence: TYRO3 Antibody [TA336749] - ICC staining of HELA cells using NBP128635 (Green) at a 1:200 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody. Nuclei were visualized using Hoechst 3325
- Quick-Filters:
- Highly Validated (10)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM06327SU-N | TYRO3 antibody |
||||||
 |
Mouse | IgG1 | 10E11 | Ascites | Hu | WB |
0.1 ml /
$380.00
|
| OriGene Technologies GmbH | |||||||
| AM06410SU-N | TYRO3 antibody |
||||||
 |
Mouse | IgG1 | 6D6F10 | Ascites | Hu | E, ICC/IF |
0.1 ml /
$380.00
|
| OriGene Technologies GmbH | |||||||
| AM11072PU-N | TYRO3 antibody |
||||||
 |
Mouse | 64CT15.6.2.3.1 | Purified | Hu | P, WB |
0.4 ml /
$415.00
|
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
|||||||
| AP14424PU-N | TYRO3 (C-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu, Ms | P, WB |
0.4 ml /
$415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308260 | TYRO3 |
||||
| Recombinant protein of human TYRO3 protein tyrosine kinase (TYRO3) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP700175 | TYRO3 (C-term DDK/His tag) |
||||
| Purified recombinant protein of human TYRO3 protein tyrosine kinase (TYRO3), with C-terminal DDK/His tag, expressed in human cells, 20 µg | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP710173 | TYRO3 (C-term DDK tag) |
||||
| Purified protein of human TYRO3 protein tyrosine kinase (TYRO3), residues 41-429aa, with C-terminal DDK tag, expressed in sf9, 20ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
SF9 cells |
20 µg /
$411.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY401899 | TYRO3 overexpression lysate |
|
 |
0.1 mg /
$295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR211050L2V | Lenti ORF particles, Tyro3 (GFP-tagged) - Mouse TYRO3 protein tyrosine kinase 3 (cDNA clone MGC:90668 IMAGE:30356080), 200 uL, >10^7 TU/mL |
|
|
200 µl /
$1,470.00
|
|
| OriGene Technologies, Inc. | ||
| MR211051L2V | Lenti ORF particles, Tyro3 (GFP-tagged) - Mouse TYRO3 protein tyrosine kinase 3 (Tyro3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
$1,720.00
|
|
| OriGene Technologies, Inc. | ||
| MR211051L1V | Lenti ORF particles, Tyro3 (Myc-DDK-tagged) - Mouse TYRO3 protein tyrosine kinase 3 (Tyro3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
$1,550.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR204843L2V | Lenti ORF particles, Tyro3 (GFP-tagged ORF) - Rat TYRO3 protein tyrosine kinase (Tyro3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
$1,720.00
|
|
| OriGene Technologies, Inc. | ||
| RR204843L1V | Lenti ORF particles, Tyro3 (Myc-DDK-tagged ORF) - Rat TYRO3 protein tyrosine kinase (Tyro3), 200 uL, >10^7 TU/mL |
|
|
200 µl /
$1,550.00
|
|
| OriGene Technologies, Inc. | ||