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Thrombopoietin antibody

Principal name

Thrombopoietin antibody

Alternative names for Thrombopoietin antibody

THPO, MGDF, C-mpl ligand, Megakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, Megakaryocyte growth and development factor

SwissProt ID

P40225 (Human), P40226 (Mouse), P42705 (Canfa), P42706 (Pig), P49745 (Rat)

Gene ID

7066 (THPO), 21832 (Thpo), 103690065 (LOC103690065), 81811

Ncbi ID

NP_000451.1, 9606, NP_001166976.1, 10090, NP_000451, NP_112395, NP_112395.1, NM_000460, NM_001177597, NP_033405, NP_001166976, NM_001173505, NM_009379, NM_031133, NM_001177598

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Rt (Rat)

Available hosts

Goat, Mouse, Rabbit, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of Thrombopoietin antibody

Thrombopoietin (TPO) is also called megakaryocyte growth and development factor (MGDF). TPO, a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels.1 Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. The human MGDF gene has been mapped to chromosome 3q26.3. 2,3 The standard product used in this kit is gene recombinant mouse TPO, consisting of 335 amino acids with the molecular mass of 35KDa. As a result of glycosylation, the molecular mass is 75KDa.

General readings

Blood. 2009 Aug 20;114(8):1655-7. Epub 2009 Jun 24.
Ann Hepatol. 2008 Jul-Sep;7(3):235-44.

Western Blot analysis of THPO expression in transfected 293T cell line (H00007066-T01) by THPO MaxPab polyclonal antibody.Lane 1: THPO transfected lysate(38.39 KDa).Lane 2: Non-transfected lysate.

H00007066-B01

Western blot analysis of THPO polyclonal antibody ( Cat # PAB2956 ) in K-562 cell line lysates ( 35 µg/lane ) . THPO ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .

PAB2956

Western Blot analysis of THPO expression in transfected 293T cell line (H00007066-T01) by THPO MaxPab polyclonal antibody.Lane 1: THPO transfected lysate(37.40 KDa).Lane 2: Non-transfected lysate.

H00007066-D01P

Western blot analysis of anti-THPO(C-term) Pab in K562 cell line lysates (35ug/lane). THPO(arrow) was detected using the purified Pab (1:60 dilution).

AP14198PU-N

Thpo polyclonal antibody ( Cat # PAB13838 ) ( 1 µg/ml ).Secondary antibody : ABC Alkaline Phosphatase Kit.Lane 1 : 100 ng mouse Thpo ( non-reduced ).Lane 2 : 100 ng mouse Thpo ( reduced ).

PAB13838

Human MCF-7; WB Suggested Anti-THPO Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; THPO antibody - middle region (ARP58734_P050) in Human MCF-7 cells using Western Blot

ARP58734_P050

Human MCF-7; WB Suggested Anti-THPO Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; THPO antibody - middle region (ARP58735_P050) in Human MCF-7 cells using Western Blot

ARP58735_P050

Western blot analysis using THPO antibody Cat.-No AM06758PU-N against human THPO (AA: 250-303) recombinant protein (Expected MW is 30 kDa)

AM06758PU-N

AM06758PU-N

Flow Cytometric analysis of MCF-7 cells using THPO antibody Cat.-No AM06758PU-N (green) and negative control (red).

AM06758PU-N

Western blot analysis of Thrombopoietin expression in HepG2 (A); mouse liver (B); rat liver (C) whole cell lysates.

CEK1563 - Mouse Thrombopoietin ELISA Kit

Representative Standard Curve

CEK1563

TP723455

TP723456

TP723457

TP723885

Anti-Thrombopoietin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

TA317418

Western blot analysis using recombinant human TPO.

TA319079

Predicted band size: 38 kDa . Positive control: 293 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

TA323653

To detect hTPO by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO.

TA328460

To detect hTPO by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TPO (TA328461) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO.

TA328460

To detect hTPO by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TPO (TA328460) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO.