Thrombopoietin antibody
Principal name
Thrombopoietin antibody
Alternative names for Thrombopoietin antibody
THPO, MGDF, C-mpl ligand, Megakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, Megakaryocyte growth and development factor
Ncbi ID
NP_000451.1, 9606, NP_001166976.1, 10090, NP_000451, NP_112395, NP_112395.1, NM_000460, NM_001177597, NP_033405, NP_001166976, NM_001173505, NM_009379, NM_031133, NM_001177598
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), ELISA (detection) (E(detection)), Paraffin Sections (P)
Background of Thrombopoietin antibody
Thrombopoietin (TPO) is also called megakaryocyte growth and development factor (MGDF). TPO, a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels.1 Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. The human MGDF gene has been mapped to chromosome 3q26.3. 2,3 The standard product used in this kit is gene recombinant mouse TPO, consisting of 335 amino acids with the molecular mass of 35KDa. As a result of glycosylation, the molecular mass is 75KDa.
General readings
Blood. 2009 Aug 20;114(8):1655-7. Epub 2009 Jun 24.
Ann Hepatol. 2008 Jul-Sep;7(3):235-44.
Western Blot analysis of THPO expression in transfected 293T cell line (H00007066-T01) by THPO MaxPab polyclonal antibody.Lane 1: THPO transfected lysate(38.39 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of THPO polyclonal antibody ( Cat # PAB2956 ) in K-562 cell line lysates ( 35 µg/lane ) . THPO ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Western Blot analysis of THPO expression in transfected 293T cell line (H00007066-T01) by THPO MaxPab polyclonal antibody.Lane 1: THPO transfected lysate(37.40 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of anti-THPO(C-term) Pab in K562 cell line lysates (35ug/lane). THPO(arrow) was detected using the purified Pab (1:60 dilution).
Thpo polyclonal antibody ( Cat # PAB13838 ) ( 1 µg/ml ).Secondary antibody : ABC Alkaline Phosphatase Kit.Lane 1 : 100 ng mouse Thpo ( non-reduced ).Lane 2 : 100 ng mouse Thpo ( reduced ).
Human MCF-7; WB Suggested Anti-THPO Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; THPO antibody - middle region (ARP58734_P050) in Human MCF-7 cells using Western Blot
Human MCF-7; WB Suggested Anti-THPO Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; THPO antibody - middle region (ARP58735_P050) in Human MCF-7 cells using Western Blot
Western blot analysis using THPO antibody Cat.-No AM06758PU-N against human THPO (AA: 250-303) recombinant protein (Expected MW is 30 kDa)
Flow Cytometric analysis of MCF-7 cells using THPO antibody Cat.-No AM06758PU-N (green) and negative control (red).
Western blot analysis of Thrombopoietin expression in HepG2 (A); mouse liver (B); rat liver (C) whole cell lysates.
Representative Standard Curve
Anti-Thrombopoietin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Western blot analysis using recombinant human TPO.
Predicted band size: 38 kDa . Positive control: 293 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
To detect hTPO by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO.
To detect hTPO by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TPO (TA328461) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO.
To detect hTPO by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TPO (TA328460) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO.
To detect hTPO by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TPO (TA328460) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO.
WB Suggested Anti-THPO Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: MCF7 cell lysate
WB Suggested Anti-THPO Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: MCF7 cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA317418 | Thrombopoietin antibody |
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| Thrombopoietin Rabbit Polyclonal (aa35-50) Antibody | |||||||
 |
Rabbit | Purified | Hu | E, P |
50 µg /
$425.00
|
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| OriGene Technologies, Inc. | |||||||
| TA319079 | Thrombopoietin antibody |
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| Rabbit polyclonal anti-THPO (TPO) antibody | |||||||
 |
Rabbit | Purified | Hu | WB |
0.1 mg /
$355.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA323653 | Thrombopoietin antibody |
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| Anti-THPO Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
$335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA328460 | Thrombopoietin antibody |
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| Biotinylated Anti-Human TPO Goat Polyclonal Antibody | |||||||
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Goat | IgG | Purified | Hu | E, WB |
50 µg /
$275.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA328461 | Thrombopoietin antibody |
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| Anti-Human TPO Goat Polyclonal Antibody | |||||||
 |
Goat | IgG | Purified | Hu | E, WB |
0.1 mg /
$275.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AM06758PU-N | Thrombopoietin antibody |
||||||
 |
Mouse | IgG2a | 1B11 | Purified | Hu | E, WB |
0.1 mg /
$410.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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| AP14198PU-N | Thrombopoietin (C-term) antibody |
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Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
$415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP723455 | Thrombopoietin (transcript variant 1) |
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| Purified recombinant protein of Human thrombopoietin (THPO), transcript variant 1. | |||||
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Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
10 µg /
$230.00
|
|
| OriGene Technologies, Inc. | |||||
| TP723456 | Thrombopoietin |
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| Purified recombinant protein of Rat thrombopoietin (Thpo). | |||||
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Rat | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
10 µg /
$230.00
|
|
| OriGene Technologies, Inc. | |||||
| TP723457 | Thrombopoietin (transcript variant 2) |
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| Purified recombinant protein of Mouse thrombopoietin (Thpo), transcript variant 2. | |||||
 |
Mouse | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
10 µg /
$230.00
|
|
| OriGene Technologies, Inc. | |||||
| TP723885 | Thrombopoietin (transcript variant 2) |
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| Purified recombinant protein of Mouse thrombopoietin (Thpo), transcript variant 2 | |||||
 |
Mouse | > 90 % Preparation: . Purity Detail: >90%, as determined by Coomassie stained SDS-PAGE. |
CHO cells |
10 µg /
$255.00
|
|
| OriGene Technologies, Inc. | |||||
ELISA Kits
| Catalog No. | ||
|---|---|---|
| EA100358 | Mouse TPO ELISA Kit |
|
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1 kit /
$465.00
|
|
| OriGene Technologies, Inc. |
Lysates
| Catalog No. | ||
|---|---|---|
| LY424704 | THPO overexpression lysate |
|
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0.1 mg /
$295.00
|
|
| OriGene Technologies, Inc. | ||
| LY432926 | THPO overexpression lysate |
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0.1 mg /
$315.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR205441L2V | Lenti ORF particles, Thpo (GFP-tagged) - Mouse thrombopoietin (Thpo), transcript variant 2, 200 uL, >10^7 TU/mL |
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200 µl /
$1,240.00
|
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| OriGene Technologies, Inc. | ||
| MR205441L1V | Lenti ORF particles, Thpo (Myc-DDK-tagged) - Mouse thrombopoietin (Thpo), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
$1,110.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR210017L2V | Lenti ORF particles, Thpo (GFP-tagged ORF) - Rat thrombopoietin (Thpo), 200 uL, >10^7 TU/mL |
|
|
200 µl /
$930.00
|
|
| OriGene Technologies, Inc. | ||
| RR210017L1V | Lenti ORF particles, Thpo (Myc-DDK-tagged ORF) - Rat thrombopoietin (Thpo), 200 uL, >10^7 TU/mL |
|
|
200 µl /
$830.00
|
|
| OriGene Technologies, Inc. | ||