Septin-2 antibody
Principal name
Septin-2 antibody
Alternative names for Septin-2 antibody
SEPT2, DIFF6, KIAA0158, NEDD5, NEDD-5, Septin2, SEPT-2, Neural precursor cell expressed developmentally down-regulated protein 5
SwissProt ID
P42208 (Mouse), P54359 (Drome), Q15019 (Human), Q2NKY7 (Bovin), Q5RA66 (Ponab), Q5ZMH1 (Chick), Q91Y81 (Rat)
Ncbi ID
9606, NP_006146, NP_001008491, NP_001008491.1, NM_006155, NM_004404, NM_001008491, NM_001008492, NP_004395, NP_001008492, NP_001153189.1, NM_010891, NM_001159717, NM_001159718, NM_001159719, NM_057148
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of Septin-2 antibody
- Kit Component:
- - KN315531G1, Sept2 gRNA vector 1 in pCas-Guide vector
- KN315531G2, Sept2 gRNA vector 2 in pCas-Guide vector
- KN315531D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Kremer BE, Adang LA, Macara IG.
Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7.
Cell. 2007 Sep 7;130(5):837-50.
PMID: 17803907
Immunofluorescence of purified MaxPab antibody to SEPT2 on HeLa cell. [antibody concentration 10 ug/ml]
SEPT2 MaxPab polyclonal antibody. Western Blot analysis of SEPT2 expression in human spleen.
Western Blot analysis of SEPT2 expression in transfected 293T cell line (H00004735-T01) by SEPT2 MaxPab polyclonal antibody.Lane 1: SEPT2 transfected lysate(39.71 KDa).Lane 2: Non-transfected lysate.
SEPT2 polyclonal antibody ( Cat # PAB11438 ) ( 0.05 µg/ml ) staining of human testis lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.
Human HepG2; WB Suggested Anti-SEPT2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate. SEPT2 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; SEPT2 antibody - middle region (ARP56661_P050) in Human HepG2 cells using Western Blot
Human 293T; Host:Rabbit. Target Name:41154. Sample Tissue:Human 293T. Antibody Dilution: 1.0ug/ml. SEPT2 is supported by BioGPS gene expression data to be expressed in HEK293T.; SEPT2 antibody - N-terminal region (ARP56660_P050) in Human 293T cells using Western Blot
Human 721_B; Host:Rabbit. Target Name:41154. Sample Tissue:Human 721_B. Antibody Dilution: 1.0ug/ml. SEPT2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; SEPT2 antibody - N-terminal region (ARP56660_P050) in Human 721_B cells using Western Blot
Human Fetal Lung; Host:Rabbit. Target Name:41154. Sample Tissue:Human Fetal Lung. Antibody Dilution: 1.0ug/ml.; SEPT2 antibody - N-terminal region (ARP56660_P050) in Human Fetal Lung cells using Western Blot
Human Cervical Cancer, Mouse, Monkey Fibroblast; . Sample Type:Â 1. Human Cervical Cancer Cell Lysate (15ug). 2. Monkey Fibroblast Cell Lysate (15ug). Primary Dilution:Â 1:1000. Secondary Antibody: goat anti-Rabbit. Secondary Dilution:Â 1:40,000. Image Submitted by:Â Â Dr. Jakob Szwedo, Dr. Lupashin's Lab. University of Arkansas for Medical Sciences. See Customer Feedback tab for detailed information.. .; SEPT2 antibody - N-terminal region (ARP56660_P050) in Human Cervical Cancer, Mouse, Monkey Fibroblast cells using Western Blot
Human HepG2; WB Suggested Anti-SEPT2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate.. SEPT2 is strongly supported by BioGPS gene expression data to be expressed in HepG2.; SEPT2 antibody - N-terminal region (ARP56660_P050) in Human HepG2 cells using Western Blot
Recombinant human SEPT2, 1-361 aa, His-tagged
Human Tonsil (formalin-fixed, paraffin-embedded) stained with SEPT2 antibody AP22579PU-N followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Breast (formalin-fixed, paraffin-embedded) stained with SEPT2 antibody AP22579PU-N followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with SEPT2 antibody AP22579PU-N followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Antibody (0.05 µg/ml) staining of Human Testis lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Western blot analysis of AP53852PU-N SEPT2 Antibody (C-term) Cat.-No AP53852PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the SEPT2 antibody detected the SEPT2 protein (arrow).
TA305762 (0.05ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of extracts from Jurkat cells and MCF-7 cells, using SEPT2 antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts of various cell lines, using SEPT2 antibody.
WB Suggested Anti-SEPT2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysateSEPT2 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells
WB Suggested Anti-SEPT2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate.SEPT2 is strongly supported by BioGPS gene expression data to be expressed in HepG2
Sample Type:??. Human Cervical Cancer Cell Lysate (15ug); 2. Monkey Fibroblast Cell Lysate (15ug); Primary Dilution:??:1000; Secondary Antibody: goat anti-Rabbit; Secondary Dilution:??:40,000;
Host:Rabbit; Target Name:41154; Sample Tissue:Human Fetal Lung; Antibody Dilution: 1.0 ug/ml
Host:Rabbit; Target Name:41154; Sample Tissue:Human 721_B; Antibody Dilution: 1.0 ug/mlSEPT2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
Host:Rabbit; Target Name:41154; Sample Tissue:Human 293T; Antibody Dilution: 1.0 ug/mlSEPT2 is supported by BioGPS gene expression data to be expressed in HEK293T
Gel: 8%SDS-PAGE,Lysate: 40 ug,,Primary antibody: (SEPT2 Antibody) at dilution 1/400 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 10S
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using (SEPT2 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP22579PU-N | Septin-2 (37-49) antibody |
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Goat | Aff - Purified | Hu, Ms, Platypus, Rb, Rt | E, P, WB |
50 µg /
$425.00
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| OriGene Technologies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
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