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Septin-2 antibody

Principal name

Septin-2 antibody

Alternative names for Septin-2 antibody

SEPT2, DIFF6, KIAA0158, NEDD5, NEDD-5, Septin2, SEPT-2, Neural precursor cell expressed developmentally down-regulated protein 5

SwissProt ID

P42208 (Mouse), P54359 (Drome), Q15019 (Human), Q2NKY7 (Bovin), Q5RA66 (Ponab), Q5ZMH1 (Chick), Q91Y81 (Rat)

Gene ID

4735 (SEPT2), 18000 (Sept2), 117515

Ncbi ID

9606, NP_006146, NP_001008491, NP_001008491.1, NM_006155, NM_004404, NM_001008491, NM_001008492, NP_004395, NP_001008492, NP_001153189.1, NM_010891, NM_001159717, NM_001159718, NM_001159719, NM_057148

Available reactivities

Hu (Human), Bov (Bovine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Bt (Bat), Can (Canine), Eq (Equine), Mky (Monkey), Por (Porcine), Prim (Primate), Rb (Rabbit), Bear

Available hosts

Mouse, Goat, Rabbit

Available applications

ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of Septin-2 antibody

Kit Component:: - KN315531G1, Sept2 gRNA vector 1 in pCas-Guide vector
- KN315531G2, Sept2 gRNA vector 2 in pCas-Guide vector
- KN315531D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:: CRISPR/CAS9 Genome Editing Manual

General readings

Kremer BE, Adang LA, Macara IG.
Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7.
Cell. 2007 Sep 7;130(5):837-50.
PMID: 17803907

Immunofluorescence of purified MaxPab antibody to SEPT2 on HeLa cell. [antibody concentration 10 ug/ml]

H00004735-B01P

SEPT2 MaxPab polyclonal antibody. Western Blot analysis of SEPT2 expression in human spleen.

H00004735-B01P

Western Blot analysis of SEPT2 expression in transfected 293T cell line (H00004735-T01) by SEPT2 MaxPab polyclonal antibody.Lane 1: SEPT2 transfected lysate(39.71 KDa).Lane 2: Non-transfected lysate.

H00004735-B01P

SEPT2 polyclonal antibody ( Cat # PAB11438 ) ( 0.05 µg/ml ) staining of human testis lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.

PAB11438

Human HepG2; WB Suggested Anti-SEPT2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate. SEPT2 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; SEPT2 antibody - middle region (ARP56661_P050) in Human HepG2 cells using Western Blot

ARP56661_P050

Human 293T; Host:Rabbit. Target Name:41154. Sample Tissue:Human 293T. Antibody Dilution: 1.0ug/ml. SEPT2 is supported by BioGPS gene expression data to be expressed in HEK293T.; SEPT2 antibody - N-terminal region (ARP56660_P050) in Human 293T cells using Western Blot

ARP56660_P050

Human 721_B; Host:Rabbit. Target Name:41154. Sample Tissue:Human 721_B. Antibody Dilution: 1.0ug/ml. SEPT2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; SEPT2 antibody - N-terminal region (ARP56660_P050) in Human 721_B cells using Western Blot

ARP56660_P050

Human Fetal Lung; Host:Rabbit. Target Name:41154. Sample Tissue:Human Fetal Lung. Antibody Dilution: 1.0ug/ml.; SEPT2 antibody - N-terminal region (ARP56660_P050) in Human Fetal Lung cells using Western Blot

ARP56660_P050

Human Cervical Cancer, Mouse, Monkey Fibroblast; . Sample Type:Â 1. Human Cervical Cancer Cell Lysate (15ug). 2. Monkey Fibroblast Cell Lysate (15ug). Primary Dilution:Â 1:1000. Secondary Antibody: goat anti-Rabbit. Secondary Dilution:Â 1:40,000. Image Submitted by:Â Â Dr. Jakob Szwedo, Dr. Lupashin's Lab. University of Arkansas for Medical Sciences. See Customer Feedback tab for detailed information.. .; SEPT2 antibody - N-terminal region (ARP56660_P050) in Human Cervical Cancer, Mouse, Monkey Fibroblast cells using Western Blot

ARP56660_P050

Human HepG2; WB Suggested Anti-SEPT2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate.. SEPT2 is strongly supported by BioGPS gene expression data to be expressed in HepG2.; SEPT2 antibody - N-terminal region (ARP56660_P050) in Human HepG2 cells using Western Blot

ARP56660_P050

Recombinant human SEPT2, 1-361 aa, His-tagged

Western blot analysis of extracts from Jurkat/MCF-7 cells, using SEPT2 Antibody. The lane on the right is treated with the synthesized peptide.

Human Tonsil (formalin-fixed, paraffin-embedded) stained with SEPT2 antibody AP22579PU-N followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

AP22579PU-N

Human Breast (formalin-fixed, paraffin-embedded) stained with SEPT2 antibody AP22579PU-N followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

AP22579PU-N

Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with SEPT2 antibody AP22579PU-N followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

AP22579PU-N

Antibody (0.05 µg/ml) staining of Human Testis lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

AP22579PU-N

Western blot analysis of AP53852PU-N SEPT2 Antibody (C-term) Cat.-No AP53852PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the SEPT2 antibody detected the SEPT2 protein (arrow).

AP53852PU-N

TP304867

TP311473

TP311650

TA305762 (0.05ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA305762

Western blot analysis of extracts from Jurkat cells and MCF-7 cells, using SEPT2 antibody.The lane on the right is treated with the synthesized peptide.

TA314243

Western blot analysis of extracts of various cell lines, using SEPT2 antibody.

TA332924

WB Suggested Anti-SEPT2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysateSEPT2 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells

TA345163

WB Suggested Anti-SEPT2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate.SEPT2 is strongly supported by BioGPS gene expression data to be expressed in HepG2

TA345164

Sample Type:??. Human Cervical Cancer Cell Lysate (15ug); 2. Monkey Fibroblast Cell Lysate (15ug); Primary Dilution:??:1000; Secondary Antibody: goat anti-Rabbit; Secondary Dilution:??:40,000;

TA345164

Host:Rabbit; Target Name:41154; Sample Tissue:Human Fetal Lung; Antibody Dilution: 1.0 ug/ml

TA345164

Host:Rabbit; Target Name:41154; Sample Tissue:Human 721_B; Antibody Dilution: 1.0 ug/mlSEPT2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells

TA345164

Host:Rabbit; Target Name:41154; Sample Tissue:Human 293T; Antibody Dilution: 1.0 ug/mlSEPT2 is supported by BioGPS gene expression data to be expressed in HEK293T

TA345164

Gel: 8%SDS-PAGE,Lysate: 40 ug,,Primary antibody: (SEPT2 Antibody) at dilution 1/400 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 10S

TA350403

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using (SEPT2 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)

TA350403

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
AP22579PU-N	Septin-2 (37-49) antibody
	Goat		Aff - Purified	Hu, Ms, Platypus, Rb, Rt	E, P, WB	50 µg / $425.00
						OriGene Technologies GmbH
+3 additional images	Highly validated antibody!
TA305762	Septin-2 (N-term) antibody
	Goat Anti-SEPT2 Antibody
	Goat		Aff - Purified	Bov, Hu, Ms, Rt	WB	0.1 mg / $360.00
						OriGene Technologies, Inc.
TA314243	Septin-2 antibody
	Rabbit polyclonal anti-SEPT2 antibody
	Rabbit	IgG	Purified	Hu, Ms	E, WB	0.1 ml / $335.00
						OriGene Technologies, Inc.
TA332924	Septin-2 antibody
	Rabbit anti-SEPT2 Polyclonal Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt		0.1 mg / $335.00
						OriGene Technologies, Inc.
TA350403	Septin-2 antibody
	Rabbit Polyclonal Anti-SEPT2 Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / $335.00
						OriGene Technologies, Inc.
+1 additional image
AP53852PU-N	Septin-2 (C-term) antibody
	Rabbit	Ig	Aff - Purified	Hu	WB	0.4 ml / $415.00
						OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP304867	Septin-2 (transcript variant 4)
	Recombinant protein of human septin 2 (SEPT2), transcript variant 4
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / $748.00
				OriGene Technologies, Inc.

TP311473	Septin-2 (transcript variant 2)
	Purified recombinant protein of Homo sapiens septin 2 (SEPT2), transcript variant 2
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / $748.00
				OriGene Technologies, Inc.

TP311650	Septin-2 (transcript variant 3)
	Purified recombinant protein of Homo sapiens septin 2 (SEPT2), transcript variant 3
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / $748.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY416834	SEPT2 overexpression lysate
		0.1 mg / $295.00
		OriGene Technologies, Inc.
LY418011	SEPT2 overexpression lysate
		0.1 mg / $295.00
		OriGene Technologies, Inc.
LY423437	SEPT2 overexpression lysate
		0.1 mg / $295.00
		OriGene Technologies, Inc.
LY423438	SEPT2 overexpression lysate
		0.1 mg / $295.00
		OriGene Technologies, Inc.
LY425241	SEPT2 overexpression lysate
		0.1 mg / $295.00
		OriGene Technologies, Inc.
LY425242	SEPT2 overexpression lysate
		0.1 mg / $295.00
		OriGene Technologies, Inc.
LY429285	SEPT2 overexpression lysate
		0.1 mg / $315.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR201625L2V	Lenti ORF particles, Sept2 (GFP-tagged ORF) - Rat septin 2 (Sept2), 200 uL, >10^7 TU/mL
		200 µl / $930.00
		OriGene Technologies, Inc.

RR201625L1V	Lenti ORF particles, Sept2 (Myc-DDK-tagged ORF) - Rat septin 2 (Sept2), 200 uL, >10^7 TU/mL
		200 µl / $830.00
		OriGene Technologies, Inc.

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