PELI1 antibody
Principal name
PELI1 antibody
Alternative names for PELI1 antibody
Protein pellino homolog 1, Pellino-1, Pellino 1, PRISM, Pellino-related intracellular-signaling molecule
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of PELI1 antibody
- Kit Component:
- - KN208197G1, PELI1 gRNA vector 1 in pCas-Guide vector
- KN208197G2, PELI1 gRNA vector 2 in pCas-Guide vector
- KN208197D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Moynagh PN. The Pellino family: IRAK E3 ligases with emerging roles in innate immune signaling. Trends Immunol. 2009; 30:33-42.
Jiang Z, Johnson J, Nie H, et al. Pellino 1 is required for interleukin-1 (IL-1)-mediated signaling through its interaction with the IL-1 receptor-associated kinase 4 (IRAK4)-IRAK-tumor necrosis factor receptor-associated factor 6 (TRAF6) complex. J. Biol. Chem. 2003; 278:10952-6
Butler MP, Hanly JA, and Moynagh PN. Kinase-active interleukin-1 receptor-associated kinases promote polyubiquitination and degradation of the Pellino family: direct evidence for Pellino proteins being ubiquitin-protein isopeptide ligases. J. Biol. Chem. 2007; 282:29729-37.
Goh ET, Arthur JS, Cheung PC, et al. Identification of the protein kinases that activate the E3 ubiquitin ligase Pellino 1 in the innate immune system. Biochem. J. 2012; 441:339-46
Human 721_B; Host:Rabbit. Target Name:PELI1. Sample Tissue:Human 721_B. Antibody Dilution: 1.0ug/ml. PELI1 is supported by BioGPS gene expression data to be expressed in 721_B.; PELI1 antibody - N-terminal region (ARP57429_P050) in Human 721_B cells using Western Blot
Human HepG2; WB Suggested Anti-PELI1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; PELI1 antibody - N-terminal region (ARP57429_P050) in Human HepG2 cells using Western Blot
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807506)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807506)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807506)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807506)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (RC208197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (RC208197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807600)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (RC208197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (RC208197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (RC208197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1.(1:2000)
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807629)(1:150)
Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-PELI1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807629)(1:150)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807629)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PELI1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807629)(1:150)
Immunohistochemical staining of paraffin-embedded Human bladder tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807629)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (RC208197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1.(1:2000)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807741)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (RC208197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1.(1:2000)
Western blot analysis of Pellino 1 in human liver tissue lysate with Pellino 1 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry of Pellino in HepG2 cells with Pellino 1 antibody at 2.5 ug/ml.
Immunofluorescence of Pellino 1 in HepG2 cells with Pellino 1 antibody at 20 ug/ml.
Immunocytochemistry of Pellino in HepG2 cells with Pellino 1 antibody at 2.5 ug/mL.
Western Blot: Pellino 1 Antibody [TA336442] - Analysis of Pellino 1 (Peli1) in human Ramos lysate (A) and mouse RAW lysate (B) using Pellino 1 antibody at 1:1000.
Immunohistochemistry-Paraffin: Pellino 1 Antibody [TA336442] - Analysis of human breast using Pellino 1 antibody at 1:100.
WB Suggested Anti-PELI1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate
Host:Rabbit; Target Name:PELI1; Sample Tissue:Human 721_B; Antibody Dilution: 1.0 ug/mlPELI1 is supported by BioGPS gene expression data to be expressed in 721_B
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA326634 | PELI1 (C-term) antibody |
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| Rabbit Polyclonal Pellino 1 Antibody | |||||||
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Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, WB |
0.1 mg /
$415.00
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA807506 | PELI1 antibody |
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| PELI1 mouse monoclonal antibody, clone OTI1D9 (formerly 1D9) | |||||||
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Mouse | IgG1 | OTI1D9 | Purified | Hu | P, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA807629 | PELI1 antibody |
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| PELI1 mouse monoclonal antibody, clone OTI13F8 | |||||||
 |
Mouse | IgG1 | OTI13F8 | Purified | Hu | P, WB |
0.1 ml /
$360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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