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PELI1 antibody

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Principal name

PELI1 antibody

Alternative names for PELI1 antibody

Protein pellino homolog 1, Pellino-1, Pellino 1, PRISM, Pellino-related intracellular-signaling molecule

SwissProt ID

Q8C669 (Mouse), Q96FA3 (Human)

Gene ID

57162 (PELI1), 67245 (Peli1)

Ncbi ID

9606, NP_065702, NP_065702.2, NM_023324, NM_020651

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of PELI1 antibody

Kit Component:
- KN208197G1, PELI1 gRNA vector 1 in pCas-Guide vector
- KN208197G2, PELI1 gRNA vector 2 in pCas-Guide vector
- KN208197D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Moynagh PN. The Pellino family: IRAK E3 ligases with emerging roles in innate immune signaling. Trends Immunol. 2009; 30:33-42.
Jiang Z, Johnson J, Nie H, et al. Pellino 1 is required for interleukin-1 (IL-1)-mediated signaling through its interaction with the IL-1 receptor-associated kinase 4 (IRAK4)-IRAK-tumor necrosis factor receptor-associated factor 6 (TRAF6) complex. J. Biol. Chem. 2003; 278:10952-6
Butler MP, Hanly JA, and Moynagh PN. Kinase-active interleukin-1 receptor-associated kinases promote polyubiquitination and degradation of the Pellino family: direct evidence for Pellino proteins being ubiquitin-protein isopeptide ligases. J. Biol. Chem. 2007; 282:29729-37.
Goh ET, Arthur JS, Cheung PC, et al. Identification of the protein kinases that activate the E3 ubiquitin ligase Pellino 1 in the innate immune system. Biochem. J. 2012; 441:339-46

ARP57429_P050 - PELI1
Human 721_B; Host:Rabbit. Target Name:PELI1. Sample Tissue:Human 721_B. Antibody Dilution: 1.0ug/ml. PELI1 is supported by BioGPS gene expression data to be expressed in 721_B.; PELI1 antibody - N-terminal region (ARP57429_P050) in Human 721_B cells using Western Blot
ARP57429_P050 - PELI1
Human HepG2; WB Suggested Anti-PELI1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; PELI1 antibody - N-terminal region (ARP57429_P050) in Human HepG2 cells using Western Blot
TA807506 - PELI1
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807506)
TA807506 - PELI1
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807506)
TA807506 - PELI1
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807506)
TA807506 - PELI1
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807506)
TA807506 - PELI1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (RC208197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1.
TA807519 - PELI1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (RC208197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1.
TA807600 - PELI1
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807600)(1:150)
TA807600 - PELI1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (RC208197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1.(1:2000)
TA807606 - PELI1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (RC208197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1.(1:2000)
TA807607 - PELI1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (RC208197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1.(1:2000)
TA807629 - PELI1
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807629)(1:150)
TA807629 - PELI1
Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-PELI1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807629)(1:150)
TA807629 - PELI1
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807629)(1:150)
TA807629 - PELI1
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PELI1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807629)(1:150)
TA807629 - PELI1
Immunohistochemical staining of paraffin-embedded Human bladder tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807629)(1:150)
TA807629 - PELI1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (RC208197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1.(1:2000)
TA807741 - PELI1
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807741)(1:150)
TA807741 - PELI1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (RC208197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1.(1:2000)
TP308197 - PELI1
TA326634 - PELI1
Western blot analysis of Pellino 1 in human liver tissue lysate with Pellino 1 antibody at (A) 1 and (B) 2 ug/mL.
TA326634 - PELI1
Immunocytochemistry of Pellino in HepG2 cells with Pellino 1 antibody at 2.5 ug/ml.
TA326634 - PELI1
Immunofluorescence of Pellino 1 in HepG2 cells with Pellino 1 antibody at 20 ug/ml.
TA326634 - PELI1
Immunocytochemistry of Pellino in HepG2 cells with Pellino 1 antibody at 2.5 ug/mL.
TA336442 - PELI1
Western Blot: Pellino 1 Antibody [TA336442] - Analysis of Pellino 1 (Peli1) in human Ramos lysate (A) and mouse RAW lysate (B) using Pellino 1 antibody at 1:1000.
TA336442 - PELI1
Immunohistochemistry-Paraffin: Pellino 1 Antibody [TA336442] - Analysis of human breast using Pellino 1 antibody at 1:100.
TA344773 - PELI1
WB Suggested Anti-PELI1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate
TA344773 - PELI1
Host:Rabbit; Target Name:PELI1; Sample Tissue:Human 721_B; Antibody Dilution: 1.0 ug/mlPELI1 is supported by BioGPS gene expression data to be expressed in 721_B
  1. Quick-Filters:
  2. Highly Validated (3)

12 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PELI1 (C-term) antibody
  Rabbit Polyclonal Pellino 1 Antibody  
Western blot analysis of Pellino 1 in human liver tissue lysate with Pellino 1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / $415.00
  OriGene Technologies, Inc.
+3 additional images

Highly validated antibody!

PELI1 antibody
  PELI1 mouse monoclonal antibody, clone OTI1D9 (formerly 1D9)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807506) Mouse IgG1 OTI1D9 Purified Hu P, WB
0.1 ml / $360.00
  OriGene Technologies, Inc.
+4 additional images

Highly validated antibody!

TrueMAB

PELI1 antibody
  PELI1 mouse monoclonal antibody, clone OTI13F8  
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807629)(1:150) Mouse IgG1 OTI13F8 Purified Hu P, WB
0.1 ml / $360.00
  OriGene Technologies, Inc.
+5 additional images

Highly validated antibody!

TrueMAB

PELI1 antibody
  Rabbit Polyclonal Pellino 1 Antibody  
Western Blot: Pellino 1 Antibody [TA336442] - Analysis of Pellino 1 (Peli1) in human Ramos lysate (A) and mouse RAW lysate (B) using Pellino 1 antibody at 1:1000. Rabbit Purified Hu, Ms P, WB
0.2 ml / $415.00
  OriGene Technologies, Inc.
+1 additional image

PELI1 antibody
  PELI1 mouse monoclonal antibody, clone OTI2H3 (formerly 2H3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (RC208197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1. Mouse IgG1 OTI2H3 Purified Hu WB
0.1 ml / $360.00
  OriGene Technologies, Inc.
 

TrueMAB

PELI1 antibody
  PELI1 mouse monoclonal antibody, clone OTI14F10 (formerly 14F10)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807600)(1:150) Mouse IgG2b OTI14F10 Purified Hu P, WB
0.1 ml / $360.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

PELI1 antibody
  PELI1 mouse monoclonal antibody, clone OTI1A8 (formerly 1A8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (RC208197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1.(1:2000) Mouse IgG1 OTI1A8 Purified Hu WB
0.1 ml / $360.00
  OriGene Technologies, Inc.
 

TrueMAB

PELI1 antibody
  PELI1 mouse monoclonal antibody, clone OTI3A9 (formerly 3A9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (RC208197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1.(1:2000) Mouse IgG1 OTI3A9 Purified Hu WB
0.1 ml / $360.00
  OriGene Technologies, Inc.
 

TrueMAB

PELI1 antibody
  PELI1 mouse monoclonal antibody, clone OTI10D1 (formerly 10D1)  
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807741)(1:150) Mouse IgG1 OTI10D1 Purified Hu P, WB
0.1 ml / $360.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PELI1
  Recombinant protein of human pellino homolog 1 (Drosophila) (PELI1)  
PELI1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Peli1 (GFP-tagged) - Mouse pellino 1 (Peli1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Peli1 (GFP-tagged) - Mouse pellino 1 (Peli1), 200 uL, >10^7 TU/mL  
200 µl / $930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Peli1 (Myc-DDK-tagged) - Mouse pellino 1 (Peli1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Peli1 (Myc-DDK-tagged) - Mouse pellino 1 (Peli1), 200 uL, >10^7 TU/mL  
200 µl / $830.00
  OriGene Technologies, Inc.
 

12 Item(s)

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12 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Mouse Lenti ORF Particle (2)
  2. Primary Antibody (9)
  3. Protein | Growth Factor (1)
Clonality
  1. Monoclonal (7)
  2. Polyclonal (2)
Target
  1. PELI1 (10)
Property
  1. C-term (1)
Reactivity
  1. Human (Hu) (9)
  2. Mouse (Ms) (2)
  3. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (9)
  2. Paraffin Sections (P) (5)
  3. Enzyme Immunoassay (E) (1)
Presentation
  1. Purified (8)
  2. Aff - Purified (1)
Host
  1. Mouse (7)
  2. Rabbit (2)
Images
  1. 1 image (10)
  2. 3+ images (3)
  3. 5+ images (2)
Images: Applications
  1. Western blot / Immunoblot (WB) (9)
  2. Paraffin Sections (P) (4)
  3. IHC (2)
  4. Immunofluorescence (IF) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (12)
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