MYB antibody
Principal name
MYB antibody
Alternative names for MYB antibody
c-Myb, Proto-oncogene c-Myb, Transcriptional activator Myb
Ncbi ID
NP_001123644.1, 9606, EAW47967, 10090, NP_001185843.1, NP_005366, NP_001123644, NP_005366.2, NM_010848, NM_005375, NM_001130172, NM_001130173, NM_001161659, NM_001161660, NM_001161658, NM_001161656
Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of MYB antibody
- Kit Component:
- - KN310567G1, Myb gRNA vector 1 in pCas-Guide vector
- KN310567G2, Myb gRNA vector 2 in pCas-Guide vector
- KN310567D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Fahl SP, Crittenden RB, Allman D, Bender TP. c-Myb is required for pro-B cell differentiation. J Immunol. 2009 Nov 1;183(9):5582-92.
PMID: 19843942
Formalin-fixed, paraffin-embedded human seminoma stained with C-myb (Cat. SM1573P) using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.
MYB polyclonal antibody (A01), Lot # 050727JC01 Western Blot analysis of MYB expression in HepG2 ( Cat # L019V1 ).
Immunohistochemistry (IHC) analysis of MYB antibody (Cat.-No.: AP06542PU-N) in paraffin-embedded human breast carcinoma tissue.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with MYB polyclonal antibody ( Cat # PAB4338 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
MYB polyclonal antibody ( Cat # PAB7308 ) ( 0.3 µg/ml ) staining of human spleen lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Immunofluorescence of purified MaxPab antibody to MYB on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of MYB expression in transfected 293T cell line (H00004602-T01) by MYB MaxPab polyclonal antibody.Lane 1: MYB transfected lysate(70.4 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of MYB expression in transfected 293T cell line (H00004602-T03) by MYB MaxPab polyclonal antibody.Lane 1: MYB transfected lysate(70.4 KDa).Lane 2: Non-transfected lysate.
Human kidney; MYB antibody - N-terminal region (ARP38611_P050) in Human kidney cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-MYB Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; MYB antibody - N-terminal region (ARP38611_P050) in Human HepG2 cells using Western Blot
Western blot using Myb polyclonal antibody ( Cat # PAB10189 ) in COS-7 cell lysates.Lane 1 : vector; Lane 2 : transfected with Myb, no treatment; Lane 3 : transfected with Myb and heat stress-treated ( incubation at 42°C for 30 minutes ) to induce Myb modification.Lane 3 shows detection of bands at 75, 98, and 125 kDa corresponding to overexpressed Myb ( arrows ) : wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively.Nitrocellulose 0.45 µm membrane was blocked for 1 hr at RT in 10% BLOTTO in PBS.The primary antibody was used at 1:500.Personal communication, J. Bies, NCI NIH, Bethesda.
Western blot using affinity purified anti-C-Myb antibody in Cos7 cell lysates: Lane 1: vector; Lane 2: transfected with c-myb, no treatment; Lane 3: transfected with c-Myb and heat stress-treated (incubation at 42οC for 30 minutes) to induce c-Myb modification. Lane 3 shows detection of bands at 75, 98, and 125kDa corresponding to overexpressed c-Myb (arrows): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively. Nitrocellulose 0.45µm membrane was blocked for 1 hr at RT in 10% BLOTTO in PBS. The primary antibody was used at 1:500.
Immunoprecipitation of MYB transfected lysate using anti-MYB MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MYB MaxPab mouse polyclonal antibody (B01) (H00004602-B01).
Western blot analysis: AP20174PU-N MYB antibody staining of Jurkat lysate at 0.5 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (IHC) analyzes of c-Myb antibody (Cat.-No.: AP21138PU-N) in paraffin-embedded human breast carcinoma tissue.
Immunohistochemistry (IHC) analyzes of c-Myb antibody (Cat.-No.: AP21138PU-N) in paraffin-embedded human breast carcinoma tissue at 1/100.
Western blot (WB) analysis of c-Myb antibody (Cat.-No.: AP21138PU-N) in extracts from 293 cells.
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence analysis of HeLa cells, using MYB antibody. The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from HUVEC cells, using MYB antibody. The lane on the right is treated with the synthesized peptide.
Western Blot: c-Myb Antibody - Samples: Lane 1: Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 / Lane 2: RT-4 / Lane 3: U-251MG sp / Lane 4: Human Plasma / Lane 5: Liver / Lane 6: Tonsil; Target weight: 85, 82, 76, 72, 41, 31 (splice variants)
Western Blot: c-Myb Antibody - Western Blot was performed using affinity purified SEQer MYB antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution).
ELISA: c-Myb Antibody - Affinity Purified
TA302544 staining of Human Colon lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA302544 (4µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with EDTA buffer pH 9, HRP-staining.
TA302544 (4µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Western blot analysis of Myb expression in 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of extracts from 293 cells, using Myb (Ab-532) antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-MYB Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate
Human kidney
Host: Rabbit; Target Name: MYB; Sample Tissue: 293T Whole Cell lysates; Antibody Dilution: 1.0 ug/ml
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Hela cells, Raji cells, Primary antibody: (MYB Antibody) at dilution 1/633, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 minutes
Host: Rabbit; Target Name: MYB; Sample Tissue: OVCAR-3 Whole Cell lysates; Antibody Dilution: 1.0ug/ml
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP09406PU-N | MYB antibody |
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Rabbit | IgG | Aff - Purified | Bonobo, Chimp, Eq, Gorilla, Hu, Mky, Ms, Op, Orang-Utan, Por, Rt | E, WB |
0.1 mg /
$515.00
|
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| OriGene Technologies GmbH | |||||||
| AP09406PU-S | MYB antibody |
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|
Rabbit | IgG | Aff - Purified | Bonobo, Chimp, Eq, Gorilla, Hu, Mky, Ms, Op, Orang-Utan, Por, Rt | E, WB |
25 µl /
$190.00
|
|
| OriGene Technologies GmbH | |||||||
| AP31017PU-N | MYB (241-254) antibody |
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Goat | IgG | Aff - Purified | Bt, Can, Elephant, Eq, Hu, Mky, Platypus, Por, Rb | P |
50 µg /
$425.00
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|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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