AP09406PU-S MYB antibody

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25 µl / $190.00
Delivery Time: 2-3 working days*
(*) valid for US customers only

Quick Overview

Rabbit anti Chimpanzee, Equine, Human, Monkey, Mouse, Opossum, Orang-Utan, Porcine, Rat MYB

Product Description for MYB

Rabbit anti Chimpanzee, Equine, Human, Monkey, Mouse, Opossum, Orang-Utan, Porcine, Rat MYB.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for MYB

Product Category Primary Antibodies
Target Category
Quantity 25 µl
Synonyms Proto-oncogene c-Myb, Transcriptional activator Myb, c-Myb
Presentation Aff - Purified
Reactivity Bonobo, Chimp, Eq, Gorilla, Hu, Mky, Ms, Op, Orang-Utan, Por, Rt
Applications E, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P06876
Immunogen:
Peptide corresponding to an internal portion of the c-Myb protein
GeneID:
17863
Application ELISA: 1/10,000 – 1/25,000.
Western Blot: 1/250 – 1/1000.
Background The transcription factor c-Myb plays an important role in the development of all hematopoietic lineages during definitive hematopoiesis. Its critical role in hematopoiesis has been first demonstrated in experiments with targeted disruption of the c-Myb gene where deletion of both alleles of the c-Myb gene was embryonally lethal due to severe anemia. c-Myb is a short-lived sequence specific DNA-binding protein that regulates transcription of several important genes involved directly in cellular processes such as proliferation, differentiation, and apoptosis. Posttranslational modifications such as phosphorylation, acetylation, ubiquitination, and conjugation of SUMO proteins are crucial for modulation of the transactivation activity of c-Myb.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Sramko M, Markus J, Kabát J, Wolff L, Bies J. Stress-induced inactivation of the c-Myb transcription factor through conjugation of SUMO-2/3 proteins. J Biol Chem. 2006 Dec 29;281(52):40065-75. Epub 2006 Oct 30. PubMed PMID: 17077080.
  2. Bies J, Markus J, Wolff L. Covalent attachment of the SUMO-1 protein to the negative regulatory domain of the c-Myb transcription factor modifies its stability and transactivation capacity. J Biol Chem. 2002 Mar 15;277(11):8999-9009. Epub 2002 Jan 4. PubMed PMID: 11779867.
Storage Store the antibody at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Storage Conditions for Trial Size:
This vial contains a relatively low volume of reagent (25 µl). To minimize loss of volume dilute 1:10 by adding 225 µl of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Shelf life: 3 month from despatch.
Format
Purification:
Affinity chromatography
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide
State:
Liquid purified Ig
Aff - Purified
Specificity
Specificity:
This antibody reacts with endogenous c-Myb protein and also SUMOylated overexpressed c-Myb protein in transfected Cos7 cells.
Species:
Rat, Mouse, Porcine, Human, Horse, Platypus, Opossum, Orangutan, Spider monkey, Bonobo, Gorilla, Chimpanzee.
Gene ID 17863

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