LDHA antibody
Principal name
LDHA antibody
Alternative names for LDHA antibody
LDH-A, L-lactate dehydrogenase A chain, LDH-M, PIG19, NY-REN-59, LDH muscle subunit, Cell proliferation-inducing gene 19 protein, Renal carcinoma antigen NY-REN-59
SwissProt ID
P00338 (Human), P00339 (Pig), P00340 (Chick), P04642 (Rat), P06151 (Mouse), P13491 (Rabit), P19858 (Bovin)
Ncbi ID
NP_001075746.1, 9606, NP_005557, NP_776524, NP_001075746, NP_001158887, NP_001158887.1, NM_001135239, NM_001165414, NP_001128711, NP_034829.1, NP_034829, NM_010699, NM_017025, NM_005566, NM_001165416, NM_001165415
Available reactivities
Available hosts
Available applications
Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Dot blot (Dot), Immunodiffusion (ID), Radioimmunoassay (R), Paraffin Sections (P), Flow Cytometry (F), Luminex (LMNX), Frozen Sections (C)
Background of LDHA antibody
Lactate dehydrogese (LDH) is a ubiquitous enzyme that catalyzes the inter-conversion of pyruvate and lactate, with simultaneous inter-conversion of DH and D+. Mammalian LDH has five tetrameric isozymes comprising combitions of two subunits (i) A subunit (LDHA or LDHM) predomites in skeletal muscle where it involve in aerobic metabolism and pyruvate reduction (ii) B subunit (LDHB) predomites in cardiac muscle where it helps catalyze aerobic oxidation of pyruvate. Several human cancers such as including rel, breast, gastric, sopharyngeal cancer etc. have higher LDHA levels compared to normal tissues and LDHA upregulation ensures efficient aerobic/glycolytic metabolism for tumor cells with reduced oxygen dependency. Moreover, LDHA also plays an important role in the development, invasion and metastasis of several maligncies and induced suppression of LDHA in cancer cells has been shown to trigger ROS burst, mitochondrial pathway apoptosis and limited tumorigenic potential. Defects in LDHA have also been linked to exertiol myoglobinuria and glycogen storage disease type 11 (GSD11).
General readings
Zhu, X., et al. Genet. Epidemiol. 34(2):171-187(2010)
Zhuang, L., et al. Mod. Pathol. 23(1):45-53(2010)
Zhao, Y.H., et al. Oncogene 28(42):3689-3701(2009)
Xie, H., et al. Mol. Cancer Ther. 8(3):626-635(2009)
Koukourakis, M.I., et al. Oncology 77(5):285-292(2009)
Rikova, K., et al. Cell 131(6):1190-1203(2007)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Immunofluorescence Microscopy of Biotin conjugated Anti-Lactate Dehydrogenase Antibody. Tissue: HeLa cells. Fixation: fixed for 5 min in 1/1 MeTOH:Acetone, blocked with (preservative free) for 15 min. Antigen retrieval: not required. Primary antibody: Lactate Dehydrogenase antibody at 1/200 for 1 h at RT. Secondary antibody: DyLight 488 conjugated Streptavidin antibody at 1/10,000 for 30 min at RT.
Staining: Lactate Dehydrogenase as green fluorescent signal.
Staining: Lactate Dehydrogenase as green fluorescent signal.
Western blot analysis shows anti-Lactate Dehydrogenase antibody detects LDH in HeLa cell extracts. Reactivity with LDH is observed in the cytoplasmic fraction (CF) and little to no reactivity in the nuclear fraction (NF). ~30 µg was loaded per lane onto a 10% gel for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kDa corresponding to the expected molecular weight for the protein. The blot was incubated with a 1:1,000 dilution of the antibody in 5% milk in TBST at room temperature followed by detection using standard procedures. Personal communication Kuldeep Patel.
Human Bronchial Epithelial Tissue; Rabbit Anti-LDHA Antibody . Catalog Number: ARP54777_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue . Observed Staining: Cytoplasmic . Primary Antibody Concentration: 1:100 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-LDHA Antibody (ARP54777_P050) ) in Human Bronchial Epithelial Tissue using Immunohistochemistry
Human Hela; Host: Rabbit . Target Name: LDHA . Sample Tissue: Hela . Antibody Dilution: 1.0ug/ml. LDHA is supported by BioGPS gene expression data to be expressed in HeLa.; LDHA antibody - middle region (ARP54777_P050) in Human Hela cells using Western Blot
Human 293T; WB Suggested Anti-LDHA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate; LDHA antibody - middle region (ARP54777_P050) in Human 293T cells using Western Blot
LDHA MaxPab rabbit polyclonal antibody. Western Blot analysis of LDHA expression in human kidney.
Western Blot analysis of LDHA expression in transfected 293T cell line (H00003939-T01) by LDHA MaxPab polyclonal antibody.Lane 1: LDHA transfected lysate(36.70 KDa).Lane 2: Non-transfected lysate.
Immunoblot analysis of cell lysates.Lane 1 : HeLa cell lysate. Lnae 2 : HepG2 cell lysate.Lane 3 : 293T cell lysate.Lane 4 : SK-N-MC cell lysate.Lane 5 :RD cell lysate.Lane 6 : PC-12 cell lysate.Lane 7 : NIH/3T3 cell lysate.
Immunoblot analysis of cell lysates.Lane 1 : HeLa cell lysate. Lnae 2 : MCF-7 cell lysate.Lane 3 : HepG2 cell lysate.Lane 4 : 293T cell lysate.Lane 5 :SK-N-MC cell lysate.Lane 6 : RD cell lysate.Lane 7 : IMR-32 cell lysate.
Human Liver; Liver; LDHA antibody - N-terminal region (ARP54776_P050) in Human Liver cells using Immunohistochemistry
Mouse; LDHA antibody - N-terminal region (ARP54776_P050) validated by WB using LdhC knockout mouse sperm, LdhA transgenic kidney, IdhA transgenic testis, LdhC knockout testis at 1:1000.; LDHA antibody - N-terminal region (ARP54776_P050) in Mouse cells using Western Blot
Human Placenta; WB Suggested Anti-LDHA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Placenta; LDHA antibody - N-terminal region (ARP54776_P050) in Human Placenta cells using Western Blot
Recombinant human LDHA, 1-332 aa, His-tagged: 15% SDS-PAGE (3 µg)
LDHA MaxPab polyclonal antibody. Western Blot analysis of LDHA expression in human liver.
Western Blot analysis of LDHA expression in transfected 293T cell line (H00003939-T01) by LDHA MaxPab polyclonal antibody.Lane 1: LDHA transfected lysate(36.52 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of LDHA expression in transfected 293T cell line (H00003939-T01) by LDHA MaxPab polyclonal antibody.Lane 1: LDHA transfected lysate(36.70 KDa).Lane 2: Non-transfected lysate.
LDHA MaxPab rabbit polyclonal antibody. Western Blot analysis of LDHA expression in human kidney.
Immunoprecipitation of LDHA transfected lysate using anti-LDHA MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with LDHA MaxPab mouse polyclonal antibody (B01) (H00003939-B01).
Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human LDHA antibody (1:500) with Texas Red (Red).
Western blot analysis: Cell lysates of HeLa (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human LDHA (1:8000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human skin tissue reacted with LDHA Antibody (Center) Cat.-No AP52459PU-N which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of LDHA Antibody (Center) Cat.-No AP52459PU-N in Ramos, A375 cell line lysates (35ug/lane). This demonstrates the LDHA antibody detected the LDHA protein (arrow).
Flow cytometry analysis of Hela cells using LDHA Antibody (Center) Cat.-No AP52459PU-N (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500518)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500518)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500518)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500518)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500518)
Immunoprecipitation(IP) of LDHA by using TrueMab monoclonal anti-LDHA antibodies (Negative control: IP without adding anti-LDHA antibody.). For each experiment, 500ul of DDK tagged LDHA overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LDHA antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LDHA (RC209378, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LDHA.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500531)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500531)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500531)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500531)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500531)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500531)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500531)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500531)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500531)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500531)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500531)
Immunoprecipitation(IP) of LDHA by using TrueMab monoclonal anti-LDHA antibodies (Negative control: IP without adding anti-LDHA antibody.). For each experiment, 500ul of DDK tagged LDHA overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LDHA antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LDHA (RC209378, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LDHA.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500558)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500558)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500558)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500558)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500558)
Immunoprecipitation(IP) of LDHA by using TrueMab monoclonal anti-LDHA antibodies (Negative control: IP without adding anti-LDHA antibody.). For each experiment, 500ul of DDK tagged LDHA overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LDHA antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LDHA (RC209378, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LDHA.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500568)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500568)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500568)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500568)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500568)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500568)
Immunoprecipitation(IP) of LDHA by using TrueMab monoclonal anti-LDHA antibodies (Negative control: IP without adding anti-LDHA antibody.). For each experiment, 500ul of DDK tagged LDHA overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LDHA antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LDHA (RC209378, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LDHA.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LDHA monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
LDHA ELISA with 5G10 Capture (TA600008) and 2B11 Detection (TA700008) Antibodies. Substrate used: Recombinant Human LDHA (TP309378)
LDHA Luminex ELISA with 5G10 Capture (TA600008) and 2B11 Detection (TA700008) Antibodies. Substrate used: Recombinant Human LDHA (TP309378)
LDHA ELISA with 5G10 Capture (TA600008) and 2B11 Detection (TA700008) Antibodies. Substrate used: Recombinant Human LDHA (TP309378)
LDHA Luminex ELISA with 5G10 Capture (TA600008) and 2B11 Detection (TA700008) Antibodies. Substrate used: Recombinant Human LDHA (TP309378)
Western blot analysis of Jurkat cell and A431 cell lysate using LDHA antibody.
Immunohistochemistry of paraffin-embedded rat lung using LDHA antibody at dilution of 1:200 (200x lens).
Anti-LDHA antibody (0.3µg/ml) staining of Heart (A), Skeletal Muscle (B) and Kidney (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Lactate Dehydrogenase A Antibody [TA336781] - Analysis of Lactate Dehydrogenase A in HeLa whole cell lysates.
Western Blot: Lactate Dehydrogenase A Antibody [TA336781] - Analysis of Lactate Dehydrogenase A in NIH/3T3 whole cell lysates.
Western Blot: Lactate Dehydrogenase A Antibody [TA336781] - Lactate Dehydrogenase A expression in bovine skeletal muscle lysate. Image from verified customer review.
Western Blot: Lactate Dehydrogenase A Antibody [TA336781] - Lactate Dehydrogenase A expression in porcine skeletal muscle lysate. Image from verified customer review.
Immunohistochemistry: Lactate Dehydrogenase A Antibody [TA336781] - IHC staining of Lactate Dehydrogenase A in lung tissue.
Immunohistochemistry: Lactate Dehydrogenase A Antibody [TA336781] - Staining of postnatal day 8(P8) Plp-EGFP transgenic mice brain section. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A Antibody [TA336781] - Immunocytochemical analysis of Lactate Dehydrogenase A in HeLa cells
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN209378BN | LDHA - human gene knockout kit via CRISPR |
||
 |
Human | mBFP-Neo |
1 kit /
$1,290.00
|
| OriGene Technologies, Inc. | |||