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Huntingtin antibody

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Principal name

Huntingtin antibody

Alternative names for Huntingtin antibody

Huntington Disease Protein, HD, IT15, HTT

SwissProt ID

P42858 (Human), P42859 (Mouse), P51111 (Rat)

Gene ID

3064 (HTT), 15194 (Htt)

Ncbi ID

NP_002102.4, 9606, NP_002102, NM_002111, NP_034544.1

Available reactivities

Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Chimp (Chimpanzee), Chk (Chicken), Can (Canine), Mky (Monkey)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Huntingtin antibody

Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which trans lates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded p olyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression. [provided by RefSeq]
Antigen distribution: Nucleus but also cytoplasmic expression in most tissues. Highest levels in neurons and paneth cells in gastrointestinal tract. Distinct granular expression pattern in several glandular epithelia.

General readings

1. Trushina,E. et al. (2004) Mutant huntingtin impairs axonal trafficking in mammalian neurons in vivo and in vitro. Mol. Cell. Biol. 24 (18), 8195-
8209.
2. Lee,W.C., Yoshihara,M. and Littleton,J.T. (2004) Cytoplasmic aggregates trap polyglutamine-containing proteins and block axonal transport in
a Drosophila model of Huntington's disease. Proc. Natl. Acad. Sci. U.S.A. 101 (9), 3224-3229.
3. Zainelli,G.M., Ross,C.A., Troncoso,J.C., Fitzgerald,J.K. and Muma,N.A. (2004) Calmodulin regulates transglutaminase 2 cross-linking of huntingtin. J. Neurosci. 24 (8), 1954-1961.

R1553P - Huntingtin
Figure 2. Immunohistochemistry. Affinity Purified anti-Huntingtin pS421 antibody was used at a 1:100 dilution to detect phosphorylated Huntingtin by immunohistochemistry in human brain cerebellum. Positive cytoplasmic staining is observed in neurons. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Alan Yen, LifeSpanBiosciences, Seattle, WA.
R1553P - Huntingtin
Figure 1. Western blot analysis is shown using Affinity Purified anti-Huntingtin pS421 antibody to detect endogenous protein present in an unstimulated human PC-3 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~190 kDa corresponding to truncated human Huntingtin protein. The blot was incubated with a 1:1,000 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication, Steven Pelech, Kinexus Inc., Vancouver, BC.
SM1660 - Huntingtin
Total protein extract of normal human cerebral cortex separated as a strip on a 3-12.5% gradient SDS-PAGE gel and Western blotted. The blot was probed with SM1660.
SM1661 - Huntingtin
Total protein extract of normal human cerebral cortex separated as a strip on a 3-12.5% gradient SDS-PAGE gel and Western blotted. The blot was probed with SM1661.
SM1662 - Huntingtin
Total protein extract of normal human cerebral cortex separated as a strip on a 3- 12.5% gradient SDS-PAGE gel and Western blotted. The blot was probed with SM1662.
H00003064-M01 - Huntingtin
Detection limit for recombinant GST tagged HD is approximately 0.03ng/ml as a capture antibody.
H00003064-M02 - Huntingtin
Detection limit for recombinant GST tagged HD is approximately 0.3ng/ml as a capture antibody.
H00003064-M05 - Huntingtin
Detection limit for recombinant GST tagged HD is approximately 0.3ng/ml as a capture antibody.
H00003064-M06 - Huntingtin
Detection limit for recombinant GST tagged HD is approximately 0.3ng/ml as a capture antibody.
H00003064-M10 - Huntingtin
Detection limit for recombinant GST tagged HD is approximately 0.3ng/ml as a capture antibody.
H00003064-M11 - Huntingtin
Immunoperoxidase of monoclonal antibody to HD on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml]
H00003064-M11 - Huntingtin
HD monoclonal antibody (M11), clone 3F1 Western Blot analysis of HD expression in Hela NE ( Cat # L013V3 ).
H00003064-M11 - Huntingtin
HD monoclonal antibody (M11), clone 3F1. Western Blot analysis of HD expression in U-2 OS ( Cat # L022V1 ).
H00003064-M11 - Huntingtin
Detection limit for recombinant GST tagged HD is approximately 0.3ng/ml as a capture antibody.
AP07574PU-N - Huntingtin
Brain cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE)
MAB0051 - Huntingtin
Western blot analysis of Huntington Disease protein in different lymphoblast cell line extracts ( Lanes 1-4 ) ( CAG repeats ~65 and ~20 ) using HD monoclonal antibody, clone 1A771 ( Cat # MAB0051 ) ( Panel A ) and an antibody from another source ( Panel B ) .
PAB10327 - Huntingtin
HTT (phospho S421) polyclonal antibody ( Cat # PAB10327 ) was used at a 1 : 100 dilution to detect phosphorylated HTT by immunohistochemistry in human cerebellum.Positive cytoplasmic staining is observed in neurons.Tissue was formalin-fixed and paraffin embedded.Personal Communication, Alan Yen, Life Span Biosciences, Seattle, WA.
PAB10327 - Huntingtin
Western blot analysis after AKT and phosphatase treatment is shown using HTT (phospho S421) polyclonal antibody ( Cat # PAB10327 ).In ( A ) untreated lysates from N2A and 293A cells were stained directly using HTT (phospho S421) polyclonal antibody ( Cat # PAB10327 ).Full length staining of HTT is noted, albeit at low levels of expression, as well as a strongly stainingband at 200 kDa that may represent staining of truncatedprotein.In B ) staining is shown after immunoprecipitation using a monoclonal antibody followed by AKT treatment ( to phosphorylate ), along with untreated, and phosphatase ( PP ) treated ( dephosphorylate ) immunoprecipitated HTT.Full length phosphorylated HTT is clearly detected in these immuno purified samples ( except dephosphorylated ).In C ) lysates are treated directly with AKT or PP to alter the phosphorylation status of HTT.Personal communication, Simon Warby, CMMT, Vancouver, BC.
PAB10327 - Huntingtin
Western blot analysis is shown using HTT (phospho S421) polyclonal antibody ( Cat # PAB10327 ) to detect endogenous protein present in an unstimulated human PC-3 wholecell lysate ( arrowhead ).Comparison to a molecular weight marker indicates a band of ~190 kDa corresponding to truncated human HTT protein.The blot was incubated witha 1 : 1,000 dilution of the antibody at room temperature followed by detection using standard techniques.Personal communication, Steven Pelech, Kinexus Inc., Vancouver, BC.
AM31065PU-N - Huntingtin
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM31065PU-N - Huntingtin
HD monoclonal antibody clone 3F1. Western Blot analysis of HD expression in U-2 OS.
AM31065PU-N - Huntingtin
Detection limit for recombinant GST tagged HD is approximately 0.3ng/ml as a capture antibody.
AM26742PU-N - Huntingtin
Immunohistochemistry on paraffin sections: Human hypothalamus, stained with AM26742PU-N.
AP33391PU-N - Huntingtin
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
TA309937 - Huntingtin
Western blot analysis of Huntingtin expression in HEK293 cells.
TA336451 - Huntingtin
Western Blot: Huntingtin Antibody (1A771) [TA336451] - Analysis of Htt in four different lymphoblast HD cell lines extracts each expressing ~65 (mutant) and ~20 (normal) CAG repeats. Lanes 1-4. A, HTT mAb clone 1A771 . B. An Htt antibody recognizing both
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Huntingtin antibody
  Mouse anti-HTT (Huntington) monoclonal antibody  
Western blot analysis of Huntingtin expression in HEK293 cells. Mouse IgG H.F5 Purified Hu, Ms P, WB
0.1 mg / $295.00
  OriGene Technologies, Inc.

Huntingtin antibody
  Rabbit polyclonal Huntingtin phospho S421 antibody (Phospho-specific)  
Huntingtin Rabbit IgG Purified Hu E, P, WB
0.1 mg / $515.00
  OriGene Technologies, Inc.

Huntingtin antibody
  Mouse Monoclonal Huntingtin Antibody (1A771)  
Western Blot: Huntingtin Antibody (1A771) [TA336451] - Analysis of Htt in four different lymphoblast HD cell lines extracts each expressing ~65 (mutant) and ~20 (normal) CAG repeats. Lanes 1-4. A, HTT mAb clone 1A771 . B. An Htt antibody recognizing both Mouse IgG 1A771 Purified Hu, Ms WB
0.1 mg / $415.00
  OriGene Technologies, Inc.

Huntingtin (85-200) antibody
Huntingtin Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
20 µl / $50.00
  SICGEN

Huntingtin (85-200) antibody
Huntingtin Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
0.6 mg / $280.00
  SICGEN

Huntingtin (1247-1646) antibody
Immunohistochemistry on paraffin sections: Human hypothalamus, stained with AM26742PU-N. Mouse IgG2b 3HU-4E6 Purified Hu, Ms P
0.1 mg / $420.00
  OriGene Technologies GmbH

Huntingtin antibody
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse 3F1 Purified Hu E, P, WB
50 µg / $475.00
  OriGene Technologies GmbH
+2 additional images

Huntingtin pSer421 antibody
Brain cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu E, P, WB
50 µg / $425.00
  OriGene Technologies GmbH

Huntingtin (C-term) antibody
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / $425.00
  OriGene Technologies GmbH

Huntingtin control peptide
Huntingtin Purified
50 µg / $205.00
  OriGene Technologies GmbH

Huntingtin control peptide
Huntingtin Purified
50 µg / $205.00
  OriGene Technologies GmbH

Huntingtin antibody
Total protein extract of normal human cerebral cortex separated as a strip on a 3- 12.5% gradient SDS-PAGE gel and Western blotted. The blot was probed with SM1661. Mouse IgG1 HDB4E10 Purified Hu, Ms, Rb C, IP, WB
0.1 mg / $410.00
  OriGene Technologies GmbH

Huntingtin antibody
Total protein extract of normal human cerebral cortex separated as a strip on a 3- 12.5% gradient SDS-PAGE gel and Western blotted. The blot was probed with SM1662. Mouse IgG1 HDC8A4 Purified Hu, Ms, Rb C, IP, WB
0.1 mg / $410.00
  OriGene Technologies GmbH

CRISPR

Catalog No. Species Donor Vector  

Htt - mouse gene knockout kit via CRISPR
Htt - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / $1,290.00
  OriGene Technologies, Inc.

Htt - mouse gene knockout kit via CRISPR
Htt - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / $1,290.00
  OriGene Technologies, Inc.

Htt - mouse gene knockout kit via CRISPR
Htt - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / $1,290.00
  OriGene Technologies, Inc.

Htt - mouse gene knockout kit via CRISPR
Htt - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / $1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

HTT overexpression lysate
HTT overexpression lysate  
0.1 mg / $495.00
  OriGene Technologies, Inc.

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