FAF1 antibody
Principal name
FAF1 antibody
Alternative names for FAF1 antibody
UBXD12, UBXN3A, CGI-03, FAS-associated factor 1,UBX domain-containing protein 3A, UBX domain-containing protein 12
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP)
Background of FAF1 antibody
Fas-associated protein 1 (FAF1) was initially identified as a Fas-binding pro-apoptotic protein that is component of the death-inducing signaling complex in Fas-mediated apoptosis (1). FAF1 can also induce apoptosis in the absence of extrinsic death signals when overexpressed although it does not contain typical death motifs such as the death domain, death effector domain, and caspase recruitment domain (1,2). Overexpression of FAF1 also decreases the basal level of NF-kappaB activity in transfected 293 cells, inhibits NF-kappaB activity induced by TNF-alpha, IL-1beta and lipopolysaccharide, and prevents NF-kappaB translocation to the nucleus (3), suggesting that another role of FAF1 is to negatively regulate the activity of NF-kappaB. FAF1 can also interact with the inflammatory signaling PYRIN-containing Apaf-1-like proteins (PYPAFs, also called NALPs) such as PYPAF1, PYPAF2 (NALP2), and PYPAF7, suggesting FAF1 may also be involved in the inflammation pathway (4). Multiple differentially spliced isoforms of FAF1 are known to exist.
Immunofluorescence of monoclonal antibody to FAF1 on HeLa cell. [antibody concentration 30 ug/ml]
FAF1 monoclonal antibody (M01), clone 1A10 Western Blot analysis of FAF1 expression in HeLa ( Cat # L013V1 ).
FAF1 polyclonal antibody (A01), Lot # 051024JC01 Western Blot analysis of FAF1 expression in Hela NE ( Cat # L013V3 ).
Immunofluorescence of purified MaxPab antibody to FAF1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of FAF1 expression in transfected 293T cell line (H00011124-T01) by FAF1 MaxPab polyclonal antibody.Lane 1: FAF1 transfected lysate(71.5 KDa).Lane 2: Non-transfected lysate.
FAF1 MaxPab polyclonal antibody. Western Blot analysis of FAF1 expression in NIH/3T3.
FAF1 MaxPab polyclonal antibody. Western Blot analysis of FAF1 expression in Y-79.
FAF1 MaxPab rabbit polyclonal antibody. Western Blot analysis of FAF1 expression in mouse testis.
FAF1 MaxPab rabbit polyclonal antibody. Western Blot analysis of FAF1 expression in human liver.
Western Blot analysis of FAF1 expression in transfected 293T cell line (H00011124-T02) by FAF1 MaxPab polyclonal antibody.Lane 1: FAF1 transfected lysate(74.00 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry of FAF1 in rat spleen tissue with FAF1 polyclonal antibody ( Cat # PAB12980 ) at 2.5 µg/ml.
Western blot analysis of FAF1 in Jurkat cell lysate with FAF1 polyclonal antibody ( Cat # PAB12980 ) at ( A ) 1 and ( B ) 2 µg/ml.
Western blot analysis of FAF1 in rat kidney tissue extract.
Human HeLa; WB Suggested Anti-FAF1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Hela Whole Cell. FAF1 is supported by BioGPS gene expression data to be expressed in HeLa.; FAF1 antibody - middle region (ARP61634_P050) in Human HeLa cells using Western Blot
Human HeLa; WB Suggested Anti-FAF1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Hela Whole Cell. FAF1 is supported by BioGPS gene expression data to be expressed in HeLa.; FAF1 antibody - C-terminal region (ARP61635_P050) in Human HeLa cells using Western Blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAF1 (RC201072, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAF1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAF1 (RC201072, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAF1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAF1 (RC201072, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAF1.
Western blot analysis of HT29 cell lysate (35ug) by using anti-FAF1 monoclonal antibody. Dilution: 1:500
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-FAF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804144)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FAF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804144)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAF1 (RC201072, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAF1.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-FAF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804153)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAF1 (RC201072, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAF1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAF1 (RC201072, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAF1.
Western blot analysis of HT29 cell lysate (35ug) by using anti-FAF1 monoclonal antibody. Dilution: 1:500
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAF1 (RC201072, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAF1.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FAF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804160)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAF1 (RC201072, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAF1.
Western blot analysis of HT29 cell lysate (35ug) by using anti-FAF1 monoclonal antibody. Dilution: 1:500
Western blot analysis of FAF1 in Jurkat cell lysate with FAF1 antibody at (A) 1 and (B) 2 ug/ml.
Immunohistochemistry of FAF1 in rat spleen tissue with FAF1 antibody at 2.5 ug/ml.
Immunofluorescence of FAF1 in rat spleen tissue with FAF1 antibody at 2 ug/mL.
Western blot analysis of FAF1 in rat kidney tissue extract.
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using FAF1 Antibody at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using FAF1 Antibody at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
Predicted band size: 74 kDa . Positive control: Mouse testis tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes)
Western blot analysis of extracts from HepG2 cells and K562 cells, using FAF1 antibody.
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA804154 | FAF1 antibody |
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| FAF1 mouse monoclonal antibody, clone OTI10D1 (formerly 10D1) | |||||||
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Mouse | IgG1 | OTI10D1 | Purified | Hu | P, WB |
0.1 ml /
$360.00
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| OriGene Technologies, Inc. | |||||||
 +15 additional images |
Highly validated antibody!
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