CD4 antibody
Principal name
CD4 antibody
Alternative names for CD4 antibody
T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
SwissProt ID
A7YY52 (Bovin), F6Y6X8, P01730 (Human), P05540 (Rat), P05542 (Sheep), P06332 (Mouse), P33705 (Canfa), P46630 (Rabit), P79355 (Felca), Q6Q4G0 (Chick), Q6R3N4
Ncbi ID
10116, NP_038516.1, NP_000607.1, NP_001001908.1, NP_001096695.1, NP_001003252.1, 9031, NP_001009250.1, 9940, NP_001075782.1, 9606, 10090, NP_000607, NP_038516, NP_036837, NP_036837.1, AAH25782, NM_013488, NM_012705
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Functional assay (FN), Flow Cytometry (F), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Luminex (LMNX)
Background of CD4 antibody
CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains.
Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin
Intracellular ligands: p56Lck
CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
General readings
1. Chan, M.L., C.-L. Chen, L. Ager, and M.D. Cooper. 1988. J. Immunol. 140:2133.
2. Cooper, M.D., R.P. Bucy, and C.-L. Chen. 1990. In: The Avian Model in Developmental Biology: From Organism to Genes. Editions du CNRS, pp. 239-249.
2. Tanimura N, Sharma JM. Appearance of T cells in the bursa of Fabricius and cecal tonsils during the acute phase of infectious bursal disease virus infection in chickens. Avian Dis. 1997;41:638-45.
3. Pantin-Jackwood MJ, Brown TP, Huff GR. Proventriculitis in broiler chickens: immunohistochemical characterization of the lymphocytes infiltrating the proventricular glands. Vet Pathol. 2004;41:641-8.
4. Abdul-Careem MK, Hunter DB, Thanthrige-Don N, Haghighi HR, Lambourne MD, Sharif S. Cellular and cytokine responses associated with dinitrofluorobenzene-induced contact hypersensitivity in the chicken. Vet Immunol Immunopathol. 2008;122:275-84.
5. Rebel JM, Peeters B, Fijten H, Post J, Cornelissen J, Vervelde L. Highly pathogenic or low pathogenic avian influenza virus subtype H7N1 infection in chicken lungs: small differences in general acute responses. Vet Res. 2011;42:10.
6. Li Z, Nestor KE, Saif YM, Fan Z, Luhtala M, Vainio O. Cross-reactive anti-chicken CD4 and CD8 monoclonal antibodies suggest polymorphism of the turkey CD8α molecule. Poult Sci. 1999;78:1526-31.
7. Sheela RR, Babu U, Mu J, Elankumaran S, Bautista DA, Raybourne RB, et al. Immune responses against Salmonella enterica serovar enteritidis infection in virally immunosuppressed chickens. Clin Diagn Lab Immunol. 2003;10:670-9.
8. Koci MD, Kelley LA, Larsen D, Schultz-Cherry S. Astrovirus-induced synthesis of nitric oxide contributes to virus control during infection. J Virol. 2004;78:1564-74.
9. Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9.
10. Tykałowski B, Stenzel T, Mikulski D, Jankowski J, Zduńczyk Z, Juśkiewicz J, et al. Level of electrolytes and percentage of T-lymphocyte subpopulations in blood of broiler chickens fed mixtures with different contents of sodium chloride. Bull Vet Inst Pulawy. 2011;55:333-7.
11. Norup LR, Dalgaard TS, Pedersen AR, Juul-Madsen HR. Assessment of Newcastle disease-specific T cell proliferation in different inbred MHC chicken lines. Scand J Immunol. 2011;74:23-30.
12. Young JA, Jefferies W. Towards the conservation of endangered avian species a recombinant West Nile Virus vaccine results in increased humoral and cellular immune responses in Japanese Quail (Coturnix japonica). PLoS One. 2013;8(6):e67137.
13. Revajová V, Slaminková Z, Grešáková L, Levkut M. Duodenal morphology and immune responses of broiler chickens fed low doses of deoxynivalenol. Acta Vet. Brno. 2013;82:337-42.
14. Xue M, Shi X, Zhao Y, Cui H, Hu S, Cui X, et al. Effects of reticuloendotheliosis virus infection on cytokine production in SPF chickens. PLoS One. 2013;8(12):e83918.
15. Peng X, Zhang K, Bai S, Ding X, Zeng Q, Yang J, et al. Histological lesions, cell cycle arrest, apoptosis and T cell subsets changes of spleen in chicken fed aflatoxin-contaminated corn. Int J Environ Res Public Health. 2014;11:8567-80.
16. Laniewski P, Kuczkowski M, Chrząstek K, Woźniak A, Wyszyńska A, Wieliczko A, et al. Evaluation of the immunogenicity of Campylobacter jejuni CjaA protein delivered by Salmonella enterica sv. Typhimurium strain with regulated delayed attenuation in chickens. World J Microbiol Biotechnol. 2014;30:281-92.
17. Kjærup RM, Dalgaard TS, Norup LR, Hamzic E, Sørensen P, Juul-Madsen HR. Characterization of cellular and humoral immune responses after IBV infection in chicken lines differing in MBL serum concentration. Viral Immunol. 2014;27:529-42.
18. Pleidrup J, Dalgaard TS, Norup LR, Permin A, Schou TW, Skovgaard K, et al. Ascaridia galli infection influences the development of both humoral and cell-mediated immunity after Newcastle Disease vaccination in chickens. Vaccine. 2014;32:383-92.
19. Dudek K, Bednarek D. Cellular immune response of pigeons in the conditions of endotoxin fever and pyrogenic tolerance. Pol J Vet Sci. 2011;14:127-33.
Table 1. Comparison of CD4 and CD8 Monoclonal antibodies performed by Flow cytometry.
Cell Source: Mouse Lymphocytes Percentage Stained above control: 14.9 %
Cell Source: Spleen Percentage of cells stained above control: 18.38%
Cell Source: CD3e Positive Spleen Cells Percentage of cells stained above control: 52.4%
Cell Source: Spleen Percentage of cells stained above control: 23.3%
Cell Source: Spleen Percentage of cells stained above control: 23.3%
Figure 1. Immunoprecipitation of Human CD4 from the lysate T cells isolated from fresh buffy coats. Western blot was immunostained by anti-Human CD4 antibody (MEM-241). Lane 1: original lysate of T cells. Lane 2: immunoprecipitate by Negative Control antibody. Lane 3: immunoprecipitate by anti-Human CD4 antibody (MEM-115).
Surface staining of human peripheral blood with anti-human CD4 (MEM-115) purified, GAM-APC.
Immunoprecipitation of human CD4 from the lysate T cells isolated from fresh buffy coats. Western blot was immunostained by anti-human CD4 (MEM-241). Lane 1: original lysate of T cells. Lane 2: immunoprecipitate by negative control antibody. Lane 3: immunoprecipitate by anti-human CD4 (MEM-115)
Surface staining of human peripheral blood with anti-human CD4 (MEM-115) purified, GAM-APC.
Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD4 (MEM-241) Er166. Gated on singlets.
Surface staining of human peripheral blood cells with anti-human CD4 (MEM-241) FITC.
Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD4 (MEM-241) Er166. Gated on singlets.
Surface staining of human peripheral blood cells with anti-human CD4 (MEM-241) FITC.
Surface staining of human peripheral blood cells with anti-human CD4 (MEM-241) FITC.
Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD4 (MEM-241) Er166. Gated on singlets.
Surface staining of human peripheral blood cells with anti-human CD4 (MEM-241) FITC.
Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD4 (MEM-241) Er166. Gated on singlets.
Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD4 (MEM-241) Er166. Gated on singlets.
Surface staining of human peripheral blood cells with anti-human CD4 (MEM-241) FITC.
Staining of mouse spleen with RAT ANTI MOUSE CD4: LOW ENDOTOXIN
Staining of mouse spleen with rat anti mouse CD4 antibody SM005P
Staining of mouse spleen cells with Rat anti Mouse CD4 (SM005PS)
Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD4
SM2180A staining of rat splenocytes.
Staining of Frozen Rat spleen with Mouse anti Rat CD4 Antibody
Staining of stimulated Rat spleen cells with Mouse Anti Rat CD4 Antibody (Cat.-No SM255P).
Staining of Frozen Rat spleen with Mouse anti Rat CD4 Antibody
Staining of stimulated Rat spleen cells with Mouse Anti Rat CD4 Antibody (Cat.-No SM255P).
Staining of Frozen Rat spleen with Mouse anti Rat CD4 Antibody
Staining of stimulated Rat spleen cells with Mouse anti Rat CD4 Antibody (Cat.-No SM255P)
Staining of bovine peripheral blood lymphocytes with MOUSE ANTI BOVINE CD4:FITC (SM435F).
Staining of rabbit peripheral blood lymphocytes with MOUSE ANTI RABBIT CD4: FITC (SM600F).
Staining of Canine peripheral blood lymphocytes with Rat Anti Canine CD4 Antibody - FITC Cat.-No SM409F
Figure 1. Peripheral blood lymphocytes stained with anti-CD4 PE labeled antibody (SM1663C).
Western blot analysis of CD4 in human thymus lysate using CD4 polyclonal antibody ( Cat # PAB0377 ) at 2 µg/ml.
( RIGHT ) Western blot analysis of CD4 ( arrow ) using rabbit CD4 polyclonal antibody ( Cat # PAB3534 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the CD4 gene ( Lane 2 ) ( Origene Technologies ) .
Western blot analysis of CD4 polyclonal antibody ( Cat # PAB3534 ) in Hela cell line lysates ( 35 µg/lane ) . CD4 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Immunoprecipitation of human CD4 from the lysate T cells isolated from fresh buffy coats. Western blot was immunostained by CD4 monoclonal antibody, clone MEM - 115 ( Cat # MAB0871 ) . Lane 1 : Original lysate of T cells. Lane 2 : Immunoprecipitate by negative control antibody. Lane 3 : immunoprecipitate by CD4 monoclonal antibody, clone MEM - 115 ( Cat # MAB0871 ) .
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD4 antibody (N-term) (AP11516PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of CD4 Antibody (N-term) in Hela cell line lysates (35 µg/lane). CD4 (arrow) was detected using the purified Pab (1:60 dilution).
Western blot analysis of CD4 (arrow) using rabbit polyclonal CD4 Antibody (N-term) (AP11516PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the CD4 gene (Lane 2) (Origene Technologies).
Human tonsil stained with anti-CD4 antibody
Human tonsil stained with Anti-CD4 Antibody Cat.-No AM11105PU (Clone SP35).
Human T cell lymphoma stained with Anti-CD4 Antibody Cat.-No AM11105PU (Clone SP35).
Human tonsil stained with Anti-CD4 Antibody Cat.-No AM11105PU (Clone SP35).
Cytometric analysis of Anti-CD4 Antibody Cat.-No AM11105PU (Clone SP35) in Jurkats (green) compare to negative control of rabbit IgG (blue).
Immunofluorescent Staining: Peripheral blood mononuclear cells from a healthy domestic Cat were double-stained with Mouse anti-Feline CD4-FITC and Mouse anti-Feline CD8-PE. Small lymphocytes were then gated and analyzed by two-color flow cytometry on a FACScan(TM) flow cytometry (BDIS, San Jose, CA). Amount Used: 1 µg/10e6 cells.
Immunofluorescent Staining: Chicken peripheral blood mononuclear cells were double stained with Mouse anti-Chicken CD4-PE and Mouse anti-Chicken CD3-FITC (AM08114FC-N). Small lymphocytes were then gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 0.1 µg/10e6 cells.
Immunofluorescent Staining: Chicken peripheral blood mononuclear cells were double stained with anti-Chicken CD4-FITC and anti-Chicken TCR alpha/beta-PE (AM08114FC-N). Small lymphocytes were then gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 µg/10e6 cells.
Western Blot analysis of CD4 expression in transfected 293T cell line (H00000920-T01) by CD4 MaxPab polyclonal antibody.Lane 1: CD4 transfected lysate(51.10 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical staining of CD4 on formalin fixed, paraffin embedded human tonsil with CD4 polyclonal antibody.
Cells from BALB/c mesenteric lymph nodes were double-stained with Cd4 monoclonal antibody, clone GK1.5 ( Cat # MAB3222 )-R-phycoerythrin and anti-mouse CD8a-FITC, gated on small lymphocytes and analyzed by flow cytometry.
Surface staining of human peripheral blood cells with CD4 monoclonal antibody, clone MEM-241 ( Cat # MAB3837 ) conjugated with FITC.
Surface staining of human peripheral blood cells with CD4 monoclonal antibody, clone MEM-241 (FITC) ( Cat # MAB4510 ).
Immunohistochemical analysis using CD4 antibody (Cat.-No. AP20210PU-N) in Paraffin-embedded human brain tissue.
Western blot analysis of CD4 (pAla429) antibody Cat.-No.- AP20210PU-N.
T-1364 CD4 (MCH class ll-restricted T cells), mouse anti human, clone EDU-2 (purified monoclonal IgG2a, lyophilized)
T-3123 CD4, mouse anti rat, clone Rib5/2 (biotinylated monoclonal IgG2a, lyophilized,)
Human Placenta; WB Suggested Anti-CD4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Placenta; CD4 antibody - C-terminal region (ARP59105_P050) in Human Placenta cells using Western Blot
Surface staining of CD4 in murine splenocytes with anti-CD4 (GK1.5) azide free, DAR/APC.
Surface staining of CD4 in murine splenocytes with anti-CD4 (GK1.5) PE.
Surface staining of CD4 in murine splenocytes with anti-CD4 (GK1.5) azide free, DAR/APC.
Surface staining of CD4 in murine splenocytes with anti-CD4 (GK1.5) PE.
Surface staining of CD4 in murine splenocytes with anti-CD4 (GK1.5) APC.
Surface staining of CD4 in murine splenocytes with anti-CD4 (GK1.5) azide free, DAR/APC.
Surface staining of CD4 in murine splenocytes with anti-CD4 (GK1.5) PE.
Surface staining of CD4 in murine splenocytes with anti-CD4 (GK1.5) azide free, DAR/APC.
Surface staining of CD4 in murine splenocytes with anti-CD4 (GK1.5) PE.
Immunohistochemistry on frozen sections: Staining with AM26053PU-N on human tonsil
Immunohistochemistry on frozen sections: Staining with AM26060BT-N on rat spleen
Courtesy of Dr. Lehmann, University of Rostock, Germany.
A typical staining pattern with the B-A1 monoclonal antibody of lymphocytes
A typical staining pattern with the B-A1 monoclonal antibody of lymphocytes
A typical staining pattern with the B-A1 monoclonal antibody of lymphocytes
A typical staining pattern with the B-A1 monoclonal antibody of lymphocytes
Staining with clone Edu-2 (CD4) monoclonal antibodies is illustrated by flow cytometry analysis of normal blood cells. Indirect staining was performed using 10 µl of the purified monoclonal antibody with RaM FITC conjugate and 100 µl blood sample.Testing by flow cytometry using a 'lyse-wash' method on whole blood from healthy donors showed the following values expressed in terms of % of the total lymphocyte count:Product code: AM39008PU-N (anti CD4 purified)Mean % positive: 45,36S.D.: 5,20% CV: 11,46
Flow Cytometric analysis of blood T cells using CD4 antibody Cat.-No AM06077PU-N (M2) and Negative Control (M1).
Flow cytometric analysis of CD4 antigen expression on human lymphocyte (left) and human monocyte (right). Open histograms indicate the reaction of Isotypic control to the cells. Shaded histograms indicate the reaction of AM26438AF-N to the cells.
BALB/c Mouse splenocytes were stained with a 0.125 µg/106 cells anti-Mouse CD4 Antibody Cat.-No AM32703PU-N (Clone GK1.5) (purple) or Negative Control (green), followed by PE-conjugated Goat anti-Rat Ig (1/200 dilution).
BALB/c Mouse splenocytes were stained with a 0.125 μg/106 cells anti-Mouse CD4 Antibody Cat.-No AM32703AF-N (Clone GK1.5) (purple) or Negative Control (green), followed by PE-conjugated Goat anti-Rat Ig (1/200 dilution).
BALB/c mouse splenocytes were stained with 0.031 µg /106 cells FITC conjugated anti-Mouse CD4 Antibody Cat.-No AM32703FC-N (Clone GK1.5) and cytocentfuged onto a glass slide prior to imaging by fluorescence microscopy (original magnification 100x)
BALB/c Mouse splenocytes were stained with a 0.031 μg/106 cells FITC conjugated anti-Mouse CD4 Antibody Cat.-No AM32703FC-N (Clone GK1.5) (purple) or Negative Control (green).
Surface staining of human peripheral blood cells with anti-human CD4 FITC (SM3020F).
Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD4 (MEM-241) Er166. Gated on singlets.
Horse peripheral blood lymphocytes stained with mouse anti horse CD4 clone CVS4 Cat.-No. SM497PS followed by FITC conjugated secondary antibody.
Horse peripheral blood lymphocytes stained with mouse anti horse CD4 clone CVS4 Cat.-No. SM497F.
Horse peripheral blood lymphocytes stained with mouse anti horse CD4 antibody clone CVS4 Cat.-No SM497R.
AM26053BT-N: human tonsil, frozen sections.
Surface staining of CD4 in murine splenocytes with anti-CD4 (GK1.5) APC.
Surface staining of CD4 in murine splenocytes with anti-CD4 (GK1.5) azide free, DAR/APC.
Surface staining of CD4 in murine splenocytes with anti-CD4 (GK1.5) PE.
Western blot analysis of CD4 expression in Jurkat (A); SP2/0 (B); PC12 (C); H9C2 (D) whole cell lysates.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD4 Antibody-Biotin labled; followed by Streptavidin PE.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD4 Antibody-APC labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD4 Antibody-APC labled.
Flow cytometric analysis of mouse spleen cells using Anti-CD4 Antibody-APC labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD4 Antibody-FITC labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD4 Antibody-FITC labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD4 Antibody-FITC labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD4 Antibody-PE labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD4 Antibody-PE labled.
Flow cytometric analysis of mouse spleen cells using Anti-CD4 Antibody-PE labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD4 Antibody-PE-Cy5 labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD4 Antibody-PE-Cy5 labled.
Flow cytometric analysis of mouse spleen cells using Anti-CD4 Antibody-PE-Cy5 labled.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD4 Antibody; followed by anti-mouse IgG PE.
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD4 Antibody; followed by anti-mouse IgG PE.
Flow cytometric analysis of mouse spleen cells using Anti-CD4 Antibody; followed by anti-mouse IgG PE.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD4 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Anti-CD4 mouse monoclonal antibody (TA500479) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD4(RC206453).
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-CD4 monoclonal antibody at 1:200.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CD4 mouse monoclonal antibody. (TA500481)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-CD4 monoclonal antibody at 1:200.
Anti-CD4 mouse monoclonal antibody (TA500482) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD4(RC206453).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
P&A Flow cytometric Analysis of live Jurkat cells, using anti-CD4 antibody (TA500483 at 1:100, Red), compared to IgG1 isotype control (Black).
Figue legend: Flow cytometric Analysis of Molt-4 cells, using anti-CD4 antibody(TA500483),(Red), compared to a nonspecific negative control antibody,(Blue). Dilution 1:50
Anti-CD4 mouse monoclonal antibody (TA500484) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD4(RC206453).
Immunofluorescent staining of COS7 cells using anti-CD4 mouse monoclonal antibody (TA500484).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CD4 mouse monoclonal antibody. (TA500488)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
ELISA: CD4 Antibody
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD4 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
ELISA: CD4 Antibody
CD4 Luminex Elisa with 6B7 Capture (TA600277) and 2D4 Detection (TA700277) Antibodies. Substrate used: Recombinant Human CD4 (TP306453)
CD4 Luminex Elisa with 6B7 Capture (TA600277) and 7F2 Detection (TA700298) Antibodies. Substrate used: Recombinant Human CD4 (TP306453)
CD4 Luminex Elisa with 6B7 Capture (TA600277) and 5D9 Detection (TA700300) Antibodies. Substrate used: Recombinant Human CD4 (TP306453)
CD4 Luminex Elisa with 7F2 Capture (TA600278) and 4A9 Detection (TA700279) Antibodies. Substrate used: Recombinant Human CD4 (TP306453)
CD4 Luminex Elisa with 4A9 Capture (TA600279) and 2H8 Detection (TA700301) Antibodies. Substrate used: Recombinant Human CD4 (TP306453)
CD4 Luminex Elisa with 4A9 Capture (TA600279) and 2A7 Detection (TA700297) Antibodies. Substrate used: Recombinant Human CD4 (TP306453)
CD4 Luminex Elisa with 4A9 Capture (TA600279) and 8H3 Detection (TA700299) Antibodies. Substrate used: Recombinant Human CD4 (TP306453)
CD4 Luminex Elisa with 8H3 Capture (TA600280) and 4A9 Detection (TA700279) Antibodies. Substrate used: Recombinant Human CD4 (TP306453)
CD4 Luminex Elisa with 5D9 Capture (TA600281) and 6B7 Detection (TA700278) Antibodies. Substrate used: Recombinant Human CD4 (TP306453)
CD4 Luminex Elisa with 2H8 Capture (TA600282) and 6B7 Detection (TA700278) Antibodies. Substrate used: Recombinant Human CD4 (TP306453)
CD4 Luminex Elisa with 6B7 Capture (TA600277) and 2D4 Detection (TA700277) Antibodies. Substrate used: Recombinant Human CD4 (TP306453)
CD4 Luminex Elisa with 5D9 Capture (TA600281) and 6B7 Detection (TA700278) Antibodies. Substrate used: Recombinant Human CD4 (TP306453)
CD4 Luminex Elisa with 2H8 Capture (TA600282) and 6B7 Detection (TA700278) Antibodies. Substrate used: Recombinant Human CD4 (TP306453)
CD4 Luminex Elisa with 7F2 Capture (TA600278) and 4A9 Detection (TA700279) Antibodies. Substrate used: Recombinant Human CD4 (TP306453)
CD4 Luminex Elisa with 8H3 Capture (TA600280) and 4A9 Detection (TA700279) Antibodies. Substrate used: Recombinant Human CD4 (TP306453)
CD4 Luminex Elisa with 4A9 Capture (TA600279) and 2A7 Detection (TA700297) Antibodies. Substrate used: Recombinant Human CD4 (TP306453)
CD4 Luminex Elisa with 6B7 Capture (TA600277) and 7F2 Detection (TA700298) Antibodies. Substrate used: Recombinant Human CD4 (TP306453)
CD4 Luminex Elisa with 4A9 Capture (TA600279) and 8H3 Detection (TA700299) Antibodies. Substrate used: Recombinant Human CD4 (TP306453)
CD4 Luminex Elisa with 6B7 Capture (TA600277) and 5D9 Detection (TA700300) Antibodies. Substrate used: Recombinant Human CD4 (TP306453)
CD4 Luminex Elisa with 4A9 Capture (TA600279) and 2H8 Detection (TA700301) Antibodies. Substrate used: Recombinant Human CD4 (TP306453)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800512)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800512)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-CD4 monoclonal antibody at 1:200.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800524)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800524)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800525)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD4 mouse monoclonal antibody. (TA800528) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800529)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD4 mouse monoclonal antibody. (TA800532) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CD4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CD4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-CD4 monoclonal antibody at 1:200.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4(RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802064)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD4 mouse monoclonal antibody. (TA802064) Dilution: 1:150
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD4 mouse monoclonal antibody. (TA802064) Dilution: 1:150
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802067)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802067)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802067)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802127)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802127)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-CD4 monoclonal antibody at 1:200.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802199)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802200)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802200)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802200)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802226)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802767)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802767)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802767)
Western blot analysis of extracts (10ug) from 1 cell line by using anti-CD4 monoclonal antibody at 1:200.
Staining of C57Bl/6 splenocytes with 0.06 ug of Rat IgG2b Isotype Control Purified (open histogram) or 0.06 ug of Anti-Mouse CD4 Purified (filled histogram) followed by Anti-Rat IgG FITC . Total viable cells were used for analysis.
Staining of C57Bl/6 thymocytes with 0.25 ug of Rat IgG2b Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD4 Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE . Cells in the lymphocyte gate were used for analysis.
Staining of C57Bl/6 splenocytes with 0.25 ug of Rat IgG2a Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD4 Purified (filled histogram) followed by Anti-Rat IgG FITC . Total viable cells were used for analysis.
Staining of normal human peripheral blood cells with 0.5 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Human CD4 Purified (filled histogram) followed by Anti-Mouse IgG FITC . Cells in the lymphocyte gate were used for analysis.
Western Blot: CD4 Antibody (B486A1) [TA336281] - Western blot analysis of human tonsil tissue using CD4 antibody at a concentration of 2 ug/ml.
Flow Cytometry: CD4 Antibody (B486A1) [TA336281] - Flow cytometric analysis of blood T cells using CD4 mouse mAb (M2) and negative control (M1).
Western Blot: CD4 Antibody [TA336736] - CD4 antibody was tested in the following cell lysates: Lane 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.
Western Blot: CD4 Antibody [TA336736] - Dog spleen homogenate. Probed with anti-CD4 and anti-CD8. Image from verified customer review.
Western Blot: CD4 Antibody [TA336736] - Mouse spleen, 25ug total protein. Image from verified customer review.
Immunohistochemistry-Paraffin: CD4 Antibody [TA336736] - Staining of paraffin-embedded mouse brain tissue. Photo courtesy of product review by verified customer.
Immunocytochemistry/Immunofluorescence: CD4 Antibody [TA336736] - CD4 antibody was tested in Jurkat cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue).
TA348948 (1 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of CD4 in human thymus tissue lysate with CD4 antibody at (A) 0.5 and (B) 1 ug/mL.
Western blot analysis of CD4 in rat lung tissue lysate with CD4 antibody at (A) 0.5 and (B) 1 ug/mL.
C57BL/6 mouse splenocytes stained with purified CD4 (clone RM4-5) (filled histogram) or purified rat IgG2a, kappa; isotype control (open histogram).
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM26053PU-N | CD4 antibody |
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 |
Mouse | IgG2a | EDU-2 | Aff - Purified | Hu | C, F |
0.1 mg /
$290.00
|
| OriGene Technologies GmbH | |||||||
Published recently! |
|||||||
| SM497PS | CD4 antibody |
||||||
 |
Mouse | IgG1 | CVS4 | Purified | Eq | C, F, IP |
0.1 mg /
$350.00
|
| OriGene Technologies GmbH | |||||||
Published recently! |
|||||||
| TA336736 | CD4 antibody |
||||||
| Rabbit Polyclonal CD4 Antibody | |||||||
![Western Blot: CD4 Antibody [TA336736] - CD4 antibody was tested in the following cell lysates: Lane 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.](https://m1.acris-antibodies.com/catalog/product/cache/2/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP1-19371-1-W.jpg) |
Rabbit | Purified | Hu, Ms, Rb, Rt | C, F, ICC/IF, P, WB |
0.1 ml /
$475.00
|
||
| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody! |
||||||
| TA336281 | CD4 antibody |
||||||
| Mouse Monoclonal CD4 Antibody (B486A1) | |||||||
![Western Blot: CD4 Antibody (B486A1) [TA336281] - Western blot analysis of human tonsil tissue using CD4 antibody at a concentration of 2 ug/ml.](https://m1.acris-antibodies.com/catalog/product/cache/2/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB100-2181-1-W.jpg) |
Mouse | IgG1 | B486A1 | Purified | Hu | C, E, F, P, WB |
0.1 ml /
$335.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AM05638PU-N | CD4 antibody |
||||||
 |
Rat | IgG2a | RM4-5 | Purified | Ms | C, F |
0.25 mg /
$460.00
|
| OriGene Technologies GmbH | |||||||
| AM05638PU-T | CD4 antibody |
||||||
|
Rat | IgG2a | RM4-5 | Purified | Ms | C, F |
25 µg /
$180.00
|
| OriGene Technologies GmbH | |||||||
| AM08113PU-N | CD4 antibody |
||||||
|
Mouse | IgG1 | 3-4F4 | Purified | Fe | C, F, IP |
0.5 mg /
$370.00
|
| OriGene Technologies GmbH | |||||||
| AM08115PU-N | CD4 antibody |
||||||
|
Mouse | IgG1 | CT-4 | Purified | Chk | C, F, FN, IP |
0.5 mg /
$370.00
|
| OriGene Technologies GmbH | |||||||
| AM08120PU-N | CD4 antibody |
||||||
|
Mouse | IgM | EP96 | Purified | Chk | C, F, FN, IP |
0.5 mg /
$370.00
|
| OriGene Technologies GmbH | |||||||
| AM08157LE-N | CD4 antibody |
||||||
|
Mouse | IgG1 | RFT-4g | Low Endotoxin | Hu | C, F, IP |
0.5 mg /
$430.00
|
| OriGene Technologies GmbH | |||||||
| AM08157PU-N | CD4 antibody |
||||||
|
Mouse | IgG1 | RFT-4g | Purified | Hu | C, F, IP |
0.1 mg /
$160.00
|
| OriGene Technologies GmbH | |||||||
| AM20691PU-N | CD4 antibody |
||||||
|
Mouse | IgG1 | CA-4 | Purified | Hu | C, ICC/IF |
0.1 mg /
$390.00
|
| OriGene Technologies GmbH | |||||||
| AM26029AF-N | CD4 antibody |
||||||
 |
Rat | IgG2b | GK1.5 | Azide Free | Ms | C, F, FN, ICC/IF, IP |
0.1 mg /
$160.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
| AM26029LE-N | CD4 antibody |
||||||
 |
Rat | IgG2b | GK1.5 | Low Endotoxin | Ms | C, F, FN, ICC/IF, IP |
0.1 mg /
$160.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
| AM26053BT-N | CD4 antibody |
||||||
 |
Mouse | IgG2a | EDU-2 | Biotin | Hu | C, F |
0.1 mg /
$330.00
|
| OriGene Technologies GmbH | |||||||
| AM26060BT-N | CD4 antibody |
||||||
 |
Mouse | IgG2a | Rib5/2 | Biotin | Rt | C, F |
0.2 mg /
$350.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AM26178PU-N | CD4 antibody |
||||||
|
Mouse | IgG1 | 15-8A2 | Purified | Rt | C |
0.1 mg /
$320.00
|
| OriGene Technologies GmbH | |||||||
| AM32703AF-N | CD4 antibody |
||||||
 |
Rat | IgG2b | GK1.5 | Azide Free | Ms | C, F, ICC/IF, IP |
0.1 mg /
$210.00
|
| OriGene Technologies GmbH | |||||||
| AM39008PU-N | CD4 antibody |
||||||
 |
Mouse | IgG2a | Edu-2 | Aff - Purified | Hu | C, F, P |
1 ml /
$270.00
|
| OriGene Technologies GmbH | |||||||
| BM4101 | CD4 antibody |
||||||
 |
Mouse | IgG2a | Rib 5/2 | Purified | Rt | C, F, FN |
0.15 mg /
$330.00
|
| OriGene Technologies GmbH | |||||||