SM497F CD4 antibody

Swiss Prot Num:

F6Y6X8

Immunogen:

Equine thymocytes

Remarks:

Spleen cells from immunised mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.

Flow cytometry: Neat-1/10, use 10µl of this working dilution to label 10e6 cells in 100µl.

Originator or purchased from resellers:

1. Goodman LB, Loregian A, Perkins GA, Nugent J, Buckles EL, Mercorelli B, et al. A point mutation in a herpesvirus polymerase determines neuropathogenicity. PLoS Pathog. 2007 Nov;3(11):e160. PubMed PMID: 17997600. (Free PMC Article available, 7 images available)
2. Go YY, Zhang J, Timoney PJ, Cook RF, Horohov DW, Balasuriya UB. Complex interactions between the major and minor envelope proteins of equine arteritis virus determine its tropism for equine CD3+ T lymphocytes and CD14+ monocytes. J Virol. 2010 May;84(10):4898-911. doi: 10.1128/JVI.02743-09. Epub 2010 Mar 10. PubMed PMID: 20219931. (Free PMC Article available, 7 images available)

1. Lunn DP, Holmes MA, Duffus WP. Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets. Immunology. 1991 Oct;74(2):251-7. PubMed PMID: 1748472. (Free PMC Article available)
2. Kydd, J.H. and Antczak, D.F., (1991) First International Workshop on Equine Leucocyte Antigens. Equine Immunol. 4 - 5.
3. Deeg CA, Reese S, Gerhards H, Wildner G, Kaspers B. The uveitogenic potential of retinal S-antigen in horses. Invest Ophthalmol Vis Sci. 2004 Jul;45(7):2286-92. PubMed PMID: 15223807.
4. Pearson W, Omar S, Clarke AF. Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses. Can J Vet Res. 2007 Jul;71(3):213-7. PubMed PMID: 17695597. (Free PMC Article available, 3 images available)
5. Brault SA, Blanchard MT, Gardner IA, Stott JL, Pusterla N, Mapes SM, et al. The immune response of foals to natural infection with equid herpesvirus-2 and its association with febrile illness. Vet Immunol Immunopathol. 2010 Sep 15;137(1-2):136-41. doi: 10.1016/j.vetimm.2010.05.010. Epub 2010 Jun 1. PubMed PMID: 20646766.
6. Hamza E, Steinbach F, Marti E. CD4+CD25+ T cells expressing FoxP3 in Icelandic horses affected with insect bite hypersensitivity. Vet Immunol Immunopathol. 2012 Jul 15;148(1-2):139-44. doi: 10.1016/j.vetimm.2011.05.033. Epub 2011 Jun 6. PubMed PMID: 21700344.
7. Lunn DP, Holmes MA, Antczak DF, Agerwal N, Baker J, Bendali-Ahcene S, et al. Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995. Vet Immunol Immunopathol. 1998 Mar 31;62(2):101-43. PubMed PMID: 9638857.

Label:

Fluorescein Isothiocyanate Isomer 1
FITC

Purification:

Affinity chromatography on Protein G from tissue culture supernatant

Buffer System:

Containing 0.09% Sodium azide and 1% BSA

State:

Liquid purified IgG
Purified

Specificity:

This anitbody reacts with T-helper cells, it recognises a 58kDa cell surface glycoprotein that is primarily expressed on a subpopulation of equine T lymphocytes and considered to be the equine homologue of human CD4.

Species:

Equine (Horse).