R1457C2 Mouse IgG (H+L chain), adsorbed antibody

Immunogen:

Mouse IgG, whole molecule

This product is designed for Immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent Western blotting.
Also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilurtions:
FLISA: 1/10,000-1/50,000.
Immunofluorescence: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.

Prior to reconstitution store at 2-8°C.
Following reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Label:

(Cyanine 2-OSu) (Molecular Weight 897 daltons)
Cy2

Absorption emission:

489 nm / 505 nm

Molar radio:

2.8 moles Cy2™ per mole of Goat IgG

Purification:

Immunoaffinity Chromatography

Buffer System:

0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2

Preservatives:

0.01% Sodium Azide

Stabilizers:

10 mg/ml BSA (IgG and Protease free)

State:

Lyophilized purified Ig fraction

Reconstitution:

Restore with 1.0 ml of deionized water (or equivalent).

Species reactivity (tested):

Mouse.

Specificity:

This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.
No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Rabbit, Rat and Sheep Serum Proteins