Beta-2-microglobulin antibody

Principal name

Beta-2-microglobulin antibody

Alternative names for Beta-2-microglobulin antibody

B2M, Beta-2 microglobulin, Beta 2 microglobulin

SwissProt ID

P01887 (Mouse), P01888 (Bovin), P07151 (Rat), P21611 (Chick), P61769 (Human), Q07717 (Pig)

Gene ID

567 (B2M), 24223 (B2m)

Available reactivities

Hu (Human), Por (Porcine), Rt (Rat), Chk (Chicken), Bov (Bovine), Ms (Mouse)

Available hosts

Mouse, Rabbit, Sheep, Goat

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Radioimmunoassay (R), Paraffin Sections (P), Immunoelectron Microscopy (EM), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of Beta-2-microglobulin antibody

ß2-microglobulin (B2M) is a principal component of the Major Histocompatibility Complex (MHC) class I molecule, a terry membrane protein complex that displays fragments derived from proteolyzed cytosolic proteins on the surface of cells for recognition by the surveillance immune system. As an integral component of the MHC class I complex,ß2-microglobulin plays a critically important role in immune system function. It has important relevance to cancer biology research; for example, research studies have shown that nearly one-third of diffuse large B cell lymphomas contain mutations that ictivate ß2-microglobulin gene function, thereby allowing tumor cells to escape immune detection. In additon,ß2-microglobulin has been identified as an amyloid preprotein with collagen-binding affinity; its accumulation in osteoarthritic lesions of long-term dialysis patients is reportedly a contributing factor to the condition known as amyloid osteoarthropathy.

General readings

1. Hilgert I, Horejsi V, Kristofova H.: The use of murine monoclonal antibody B2M-01 for
detection and purification of human beta 2-microglobulin. Folia Biol (Praha). 1984;30(6):369-76.
2. Drbal K, Angelisova P, Hilgert I, Cerny J, Novak P, Horejsi V.: A proteolytically truncated
form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated
myeloid cells. Blood. 2001 Sep 1;98(5):1561-6.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Beta-2-microglobulin antibody

  Rabbit anti-B2M Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using B2M antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / $335.00
  OriGene Technologies, Inc.

Beta-2-microglobulin (65-79) antibody

B2M antibody Cat.-No AP31992PU-N staining of Human Duodenum lysate at 0.03 μg/ml (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu E, WB
0.1 mg / $380.00
  OriGene Technologies GmbH

Beta-2-microglobulin (N-term) antibody

  Goat Anti-beta-2-microglobulin (B2M) Antibody  
(0.3ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu E, WB
0.1 mg / $360.00
  OriGene Technologies, Inc.

Beta-2-microglobulin antibody

  Rabbit polyclonal Beta-2-Microglobulin antibody  
IHC of Rabbit anti-Beta-2-Microglobulin Antibody. Tissue: normal human skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Left panel: isotype control, Right panel: ß2 microglobulin antibody at 1 ug/ml for 20 min at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ß2 microglobulin is cell membrane (and to lesser amount cytoplasmatic compartment). Rabbit Purified Hu C, E, ICC/IF, P, WB
0.1 mg / $515.00
  OriGene Technologies, Inc.

Beta-2-microglobulin antibody

  Rabbit Polyclonal B2M Antibody  
Western blot analysis of B2M in SK-N-SH cell lysate with B2M antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Purified Hu WB
0.1 mg / $415.00
  OriGene Technologies, Inc.

Beta-2-microglobulin antibody

  Anti-B2M Rabbit Polyclonal Antibody  
Predicted band size: 14 kDa . Positive control: Raji cell and human thyroid cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 15%SDS-PAGE Lane1: Raji cell lysate Lane2: Human thyroid cancer tissue lysate Lysate:s 40 ug per lane Primary antibody: 1/125 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / $335.00
  OriGene Technologies, Inc.

Beta-2-microglobulin antibody

  Anti-B2M rabbit polyclonal antibody  
Predicted band size: 14 kDa . Positive control: Human kidney tissue and Raji cell lysate. Recommended dilution: 1/200-1000. (Gel: 15%SDS-PAGE Lane1: Human kidney tissue lysate Lane2: Raji cell lysate Lysates: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / $335.00
  OriGene Technologies, Inc.

Beta-2-microglobulin (N-term) antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with B2M antibody (N-term) (Cat.#AP12414PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P
0.4 ml / $415.00
  OriGene Technologies GmbH

Beta-2-microglobulin (C-term) antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with B2M antibody (C-term) (Cat.#AP12415PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P
0.4 ml / $415.00
  OriGene Technologies GmbH

Beta-2-microglobulin antibody

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Hu E, P
0.25 mg / $425.00
  OriGene Technologies GmbH

Beta-2-microglobulin antibody

Beta-2-microglobulin Rabbit Biotin Hu C, E, P, WB
0.1 mg / $515.00
  OriGene Technologies GmbH

Beta-2-microglobulin antibody

Beta-2-microglobulin Rabbit HRP Hu C, E, EM, WB
0.5 mg / $590.00
  OriGene Technologies GmbH

Beta-2-microglobulin antibody

Beta-2-microglobulin Rabbit HRP Hu C, E, P, WB
0.1 mg / $515.00
  OriGene Technologies GmbH

13 Item(s)

per page
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