BM604HRP Beta-2-microglobulin antibody

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0.1 mg / $280.00
Delivery Time: 10 working days*
(*) valid for US customers only

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Mouse anti Human Beta-2-microglobulin B2M-01

BM604HRP

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  • BM604HRP
  • BM604HRP

Product Description for Beta-2-microglobulin

Mouse anti Human Beta-2-microglobulin B2M-01.
Presentation: HRP
Product is tested for Western blot / Immunoblot, Enzyme Immunoassay.

Properties for Beta-2-microglobulin

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms B2M, Beta 2 microglobulin, Beta-2 microglobulin
Presentation HRP
Reactivity Hu
Applications E, WB
Clonality Monoclonal
Clone B2M-01
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P61769
Immunogen:
Purified Human beta-2-Microglobulin
GeneID:
567
Application The antibody is designed for quantitative determination of soluble beta 2-Microglobulin/MHC Class I complexes. This antibody can also be used for detection of free beta 2-Microglobulin by Western blotting.
ELISA.
Western blot (Both Reducing and non-Reducing conditions):
Recommended Dilution: 2-4 µg/ml.
Positive Control: U937 Human histiocytic lymphoma cell line, RAJI human Burkitt lymphoma cell line.
Negative Control: EL4 mouse lymphoblastic lymphoma cell line.
Sample Preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer.
Background b2M non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Concentration 1.0 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Hilgert I, Horejsí V, Kristofová H. The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha). 1984;30(6):369-76. PubMed PMID: 6394369.
  2. Khurana M, Traum AZ, Aivado M, Wells MP, Guerrero M, Grall F, et al. Urine proteomic profiling of pediatric nephrotic syndrome. Pediatr Nephrol. 2006 Sep;21(9):1257-65. Epub 2006 Jun 30. PubMed PMID: 16810512. (Free PMC Article available, 5 images available)
  3. Poláková K, Železníková T, Russ G. HLA-G5 in the blood of leukemia patients and healthy individuals. Leuk Res. 2013 Feb;37(2):139-45. doi: 10.1016/j.leukres.2012.10.019. Epub 2012 Nov 10. PubMed PMID: 23146557.
  4. Lafon M, Prehaud C, Megret F, Lafage M, Mouillot G, Roa M, et al. Modulation of HLA-G expression in human neural cells after neurotropic viral infections. J Virol. 2005 Dec;79(24):15226-37. PubMed PMID: 16306594. (Free PMC Article available, 6 images available)
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
This product is photosensitive and should be protected from light.
Should it contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.
Format
Label:
Conjugated with Horseradish Peroxidase of high specific activity and RZ=3.
The reagent is free of unconjugated as determined by size-exclusion chromatography.
HRP
Buffer System:
PBS
Preservatives:
0.01% (w/v) Thimerosal
State:
Liquid purified Ig fraction
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
The antibody reacts with beta-2-Microglobulin (B2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta-2-Microglobulin.
Negative Species: Mouse, Bovine, Canine (Dog), Rabbit and Chicken.
Gene ID 567

Accessory Products

Proteins and/or Positive Controls

Proteins for Beta-2-microglobulin (16 products)

Catalog No. Species Pres. Purity   Source  

Beta-2-microglobulin

Beta-2-microglobulin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

Beta-2-microglobulin (C-term DDK tag)

Beta-2-microglobulin Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / $411.00
  OriGene Technologies, Inc.

Beta-2-microglobulin

Beta-2-microglobulin Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / $299.00
  OriGene Technologies, Inc.

Beta-2-microglobulin

Beta-2-microglobulin Human Purified
  OriGene Technologies GmbH

Beta-2-microglobulin

Beta-2-microglobulin Human Purified
  OriGene Technologies GmbH

Beta-2-microglobulin

Beta-2-microglobulin Human Purified
  OriGene Technologies GmbH

Beta-2-microglobulin

Beta-2-microglobulin Human Purified > 95.0% as determined by by both RP-HPLC and SDS-PAGE E. coli
  OriGene Technologies GmbH

Beta-2-microglobulin

Beta-2-microglobulin Human Purified > 95.0% as determined by by both RP-HPLC and SDS-PAGE E. coli
  OriGene Technologies GmbH

Beta-2-microglobulin

Beta-2-microglobulin Human Purified > 95.0% as determined by by both RP-HPLC and SDS-PAGE E. coli
  OriGene Technologies GmbH

Beta-2-microglobulin (21-119, His-tag)

Beta-2-microglobulin Human Purified > 95 % by SDS - PAGE E. coli
50 µg / $820.00
  OriGene Technologies GmbH

Beta-2-microglobulin (21-119, His-tag)

Beta-2-microglobulin Human Purified > 95 % by SDS - PAGE E. coli
10 µg / $320.00
  OriGene Technologies GmbH

Beta-2-microglobulin

Beta-2-microglobulin Human Purified > 95 % as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
E. coli
  OriGene Technologies GmbH

Beta-2-microglobulin

Beta-2-microglobulin Human Purified > 95 % as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
E. coli
  OriGene Technologies GmbH

Beta-2-microglobulin

Beta-2-microglobulin Human Purified > 95 % as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
E. coli
  OriGene Technologies GmbH

Beta-2-microglobulin

Beta-2-microglobulin Human > 98 % Urine
  BBI Solutions

Beta-2-microglobulin

Beta-2-microglobulin Human Purified > 40 % Urine
  BBI Solutions
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