Beta-2-microglobulin antibody

Principal name

Beta-2-microglobulin antibody

Alternative names for Beta-2-microglobulin antibody

B2M, Beta-2 microglobulin, Beta 2 microglobulin

SwissProt ID

P01887 (Mouse), P01888 (Bovin), P07151 (Rat), P21611 (Chick), P61769 (Human), Q07717 (Pig)

Gene ID

567 (B2M), 24223 (B2m)

Available reactivities

Hu (Human), Por (Porcine), Rt (Rat), Chk (Chicken), Bov (Bovine), Ms (Mouse)

Available hosts

Mouse, Rabbit, Sheep, Goat

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Radioimmunoassay (R), Paraffin Sections (P), Immunoelectron Microscopy (EM), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of Beta-2-microglobulin antibody

ß2-microglobulin (B2M) is a principal component of the Major Histocompatibility Complex (MHC) class I molecule, a terry membrane protein complex that displays fragments derived from proteolyzed cytosolic proteins on the surface of cells for recognition by the surveillance immune system. As an integral component of the MHC class I complex,ß2-microglobulin plays a critically important role in immune system function. It has important relevance to cancer biology research; for example, research studies have shown that nearly one-third of diffuse large B cell lymphomas contain mutations that ictivate ß2-microglobulin gene function, thereby allowing tumor cells to escape immune detection. In additon,ß2-microglobulin has been identified as an amyloid preprotein with collagen-binding affinity; its accumulation in osteoarthritic lesions of long-term dialysis patients is reportedly a contributing factor to the condition known as amyloid osteoarthropathy.

General readings

1. Hilgert I, Horejsi V, Kristofova H.: The use of murine monoclonal antibody B2M-01 for
detection and purification of human beta 2-microglobulin. Folia Biol (Praha). 1984;30(6):369-76.
2. Drbal K, Angelisova P, Hilgert I, Cerny J, Novak P, Horejsi V.: A proteolytically truncated
form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated
myeloid cells. Blood. 2001 Sep 1;98(5):1561-6.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Beta-2-microglobulin antibody

Surface staining of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) PE. Mouse IgG2a B2M-01 Biotin Hu F
0.1 mg / $260.00
  OriGene Technologies GmbH

Beta-2-microglobulin antibody

Western Blotting analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-Human β2-microglobulin antibody (B2M-01). 
Lane 1: RAJI Human Burkitt lymphoma cell line.
Lane 2: EL4 Mouse lymphoblastic lymphoma cell line. 
Lane 3: U937 Human histiocytic lymphoma cell line. Mouse IgG2a B2M-01 HRP Hu E, WB
0.1 mg / $280.00
  OriGene Technologies GmbH

Beta-2-microglobulin antibody

  B2M mouse monoclonal antibody, clone OTI1A9 (formerly 1A9)  
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-B2M mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802776) Mouse IgG1 OTI1A9 Purified Hu E, P, WB
0.1 ml / $360.00
  OriGene Technologies, Inc.

TrueMAB

Beta-2-microglobulin antibody

1. Murine 4T1 cells were mixed with human DU-145 cells and stained with 1 µg Beta-2-microglobulin antibody (BM604S). Anti-mouse Cy3 was used as a secondary antibody. Red stained cells mark the Beta-2-microglobulin positive human DU-145 cells.
2. Murine 4T1 cells were stained with 1 µg Beta-2-microglobulin antibody (BM604S). Anti-mouse Cy3 was used as a secondary antibody. Murine 4T1 cells are negative for Beta-2-microglobulin staining.
Pictures by courtesy of Fraunhofer ITEM, Regensburg, Germany. Mouse IgG2a B2M-01 Purified Hu E, F, ICC/IF, IP, P, R, WB
0.1 mg / $210.00
  OriGene Technologies GmbH

Beta-2-microglobulin antibody

  B2M / Beta 2 Microglobulin Mouse Monoclonal (2213) Antibody  
Anti-B2M antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Mouse IgG1 2213 Purified Hu E
50 µl / $425.00
  OriGene Technologies, Inc.

Beta-2-microglobulin antibody

  B2M mouse monoclonal antibody, clone OTI1A7 (formerly 1A7)  
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-B2M mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803617) Mouse IgG1 OTI1A7 Purified Hu E, P, WB
0.1 ml / $360.00
  OriGene Technologies, Inc.

TrueMAB

Beta-2-microglobulin antibody

Methanol-fixed Hela cells stained with 20 μg/ml mouse anti-B2MG mAb clone D6E4 and fluorescence labelled secondary antibody. Mouse IgG1 D6E4 Aff - Purified Hu E, ICC/IF
0.5 mg / $470.00
  OriGene Technologies GmbH

Beta-2-microglobulin antibody

Staining of methanol-fixed Hela cells by Immunocytochemistry using Mouse anti-Human beta 2 Microglobulin monoclonal antibody (D2E9) at 1/50 (20 µg/ml). Mouse IgG1 D2E9 Aff - Purified Hu E, E(capture), F, ICC/IF, WB
0.5 mg / $470.00
  OriGene Technologies GmbH

Beta-2-microglobulin antibody

Beta-2-microglobulin Mouse IgG1 F21-21 Biotin Chk, Tr F
0.5 mg / $450.00
  OriGene Technologies GmbH

Beta-2-microglobulin antibody

Immunofluorescent Staining: Chicken peripheral blood mononuclear cells were double-stained with Mouse anti-Chicken Beta-2-Microglobulin-FITC and Mouse anti-Chicken CD3-PE (Cat#AM08114FC-N). Small lymphocytes were then gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 µg/10e6 cells. Mouse IgG1 F21-21 FITC Chk, Tr F
0.5 mg / $500.00
  OriGene Technologies GmbH

Beta-2-microglobulin antibody

Beta-2-microglobulin Mouse IgG1 F21-21 Purified Chk, Tr C, F, IP, WB
0.5 mg / $370.00
  OriGene Technologies GmbH

Beta-2-microglobulin antibody

Beta-2-microglobulin Mouse IgG1 F21-21 PE Chk, Tr F
0.1 mg / $340.00
  OriGene Technologies GmbH

Beta-2-microglobulin antibody

Detect recombinant human B2MG antigen (100ng/lane) using anti-human B2MG mAb D6D1 at 1μg/mL dilution. Mouse IgG1 D6D1 Aff - Purified Hu E, WB
0.5 mg / $470.00
  OriGene Technologies GmbH

Beta-2-microglobulin antibody

Stainning of methanol-fixed Hela cells: Mouse anti-Human beta 2 Microglobulin monoclonal antibody (D2A10) 1/50 (20 µg/ml). Mouse IgG1 D2A10 Aff - Purified Hu E, F, ICC/IF, WB
0.5 mg / $470.00
  OriGene Technologies GmbH

Beta-2-microglobulin antibody

Staining of methanol-fixed Hela cells by Immunocytochemistry: Mouse anti-Human beta 2 Microglobulin monoclonal antibody (D3A2) 1:50 (20 µg/ml). Mouse IgG1 D3A2 Aff - Purified Hu E, E(detection), F, ICC/IF, WB
0.5 mg / $470.00
  OriGene Technologies GmbH

Beta-2-microglobulin antibody

Beta-2-microglobulin Mouse IgG1 D2E9 HRP E
0.2 ml / $510.00
  OriGene Technologies GmbH

Beta-2-microglobulin antibody

Human Prostate (formalin-fixed, paraffin-embedded) stained with B2M antibody AM20523PU-N at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG1 n.a Purified Hu P
50 µg / $425.00
  OriGene Technologies GmbH

Beta-2-microglobulin antibody

Beta-2-microglobulin Mouse IgG1 2213 Purified Hu C, E, R
1 ml / $560.00
  OriGene Technologies GmbH

Beta-2-microglobulin antibody

Surface staining of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) FITC. Mouse IgG2a B2M-01 FITC Hu F, ICC/IF
0.1 mg / $260.00
  OriGene Technologies GmbH

Beta-2-microglobulin antibody

Western blot analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human β2-microglobulin (B2M-01).
Lane 1: RAJI human Burkitt lymphoma cell line
Lane 2: EL4 mouse lymphoblastic lymphoma cell line
Lane 3: U937 human histiocytic lymphoma cell line Mouse IgG2a B2M-01 PE Hu F
0.1 mg / $310.00
  OriGene Technologies GmbH

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