Beta-2-microglobulin antibody
Principal name
Beta-2-microglobulin antibody
Alternative names for Beta-2-microglobulin antibody
B2M, Beta-2 microglobulin, Beta 2 microglobulin
SwissProt ID
P01887 (Mouse), P01888 (Bovin), P07151 (Rat), P21611 (Chick), P61769 (Human), Q07717 (Pig)
Ncbi ID
NP_004039.1, NP_036644.1, 9606, 9031, NP_004039, NP_036644, NM_004048, NM_012512
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Radioimmunoassay (R), Paraffin Sections (P), Immunoelectron Microscopy (EM), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), EPair for Elisa (EPair)
Background of Beta-2-microglobulin antibody
ß2-microglobulin (B2M) is a principal component of the Major Histocompatibility Complex (MHC) class I molecule, a terry membrane protein complex that displays fragments derived from proteolyzed cytosolic proteins on the surface of cells for recognition by the surveillance immune system. As an integral component of the MHC class I complex,ß2-microglobulin plays a critically important role in immune system function. It has important relevance to cancer biology research; for example, research studies have shown that nearly one-third of diffuse large B cell lymphomas contain mutations that ictivate ß2-microglobulin gene function, thereby allowing tumor cells to escape immune detection. In additon,ß2-microglobulin has been identified as an amyloid preprotein with collagen-binding affinity; its accumulation in osteoarthritic lesions of long-term dialysis patients is reportedly a contributing factor to the condition known as amyloid osteoarthropathy.
General readings
1. Hilgert I, Horejsi V, Kristofova H.: The use of murine monoclonal antibody B2M-01 for
detection and purification of human beta 2-microglobulin. Folia Biol (Praha). 1984;30(6):369-76.
2. Drbal K, Angelisova P, Hilgert I, Cerny J, Novak P, Horejsi V.: A proteolytically truncated
form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated
myeloid cells. Blood. 2001 Sep 1;98(5):1561-6.
Surface staining of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) PE.
Surface staining of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) FITC.
Western Blotting analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human β2-microglobulin (B2M-01).
Lane 1: RAJI human Burkitt lymphoma cell line
Lane 2: EL4 mouse lymphoblastic lymphoma cell line
Lane 3: U937 human histiocytic lymphoma cell line
Lane 1: RAJI human Burkitt lymphoma cell line
Lane 2: EL4 mouse lymphoblastic lymphoma cell line
Lane 3: U937 human histiocytic lymphoma cell line
Surface staining of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) PE.
Surface staining of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) FITC.
Western Blotting analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human β2-microglobulin (B2M-01).
Lane 1: RAJI human Burkitt lymphoma cell line
Lane 2: EL4 mouse lymphoblastic lymphoma cell line
Lane 3: U937 human histiocytic lymphoma cell line
Lane 1: RAJI human Burkitt lymphoma cell line
Lane 2: EL4 mouse lymphoblastic lymphoma cell line
Lane 3: U937 human histiocytic lymphoma cell line
Western Blotting analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-Human β2-microglobulin antibody (B2M-01). Lane 1: RAJI Human Burkitt lymphoma cell line.Lane 2: EL4 Mouse lymphoblastic lymphoma cell line. Lane 3: U937 Human histiocytic lymphoma cell line.
Surface staining of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) PE.
Surface staining of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) FITC.
1. Murine 4T1 cells were mixed with human DU-145 cells and stained with 1 µg Beta-2-microglobulin antibody (BM604S). Anti-mouse Cy3 was used as a secondary antibody. Red stained cells mark the Beta-2-microglobulin positive human DU-145 cells.2. Murine 4T1 cells were stained with 1 µg Beta-2-microglobulin antibody (BM604S). Anti-mouse Cy3 was used as a secondary antibody. Murine 4T1 cells are negative for Beta-2-microglobulin staining.Pictures by courtesy of Fraunhofer ITEM, Regensburg, Germany.
Surface staining of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) PE.
Surface staining of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) FITC.
Confocal microscopy of Hman Beta-2-microglobulin-transfected canine cells labeled A) with B2M-01 antibody Alexa Fluor® 647 conjugate and B) with Alexa Fluor® 647 conjugated Mouse IgG2a Isotype Control. Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2 µg/ml.
Western Blotting analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human β2-microglobulin (B2M-01). Lane 1: RAJI human Burkitt lymphoma cell line. Lane 2: EL4 mouse lymphoblastic lymphoma cell line. Lane 3: U937 human histiocytic lymphoma cell line.
Surface staining of human peripheral blood cells by mouse monoclonal anti-beta2-microglobulin antibody B2M-02.
Surface staining of human peripheral blood cells by mouse monoclonal anti-beta2-microglobulin antibody B2M-02.
Immunoperoxidase of monoclonal antibody to B2M on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3 ug/ml]
Proximity Ligation Analysis (PLA) of protein-protein interactions between CALR and B2M. HeLa cells were stained with anti-CALR rabbit purified polyclonal 1:1200 and anti-B2M mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Immunofluorescence of monoclonal antibody to B2M on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of B2M expression in transfected 293T cell line by B2M monoclonal antibody (M01), clone 3F9-2C2.Lane 1: B2M transfected lysate(13.7 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of B2M over-expressed 293 cell line, cotransfected with B2M Validated Chimera RNAi ( Cat # H00000567-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with B2M monoclonal antibody (M01), clone 3F9-2C2 (Cat # H00000567-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
B2M monoclonal antibody (M01), clone 3F9-2C2 Western Blot analysis of B2M expression in U-2 OS ( Cat # L022V1 ).
Detection limit for recombinant GST tagged B2M is approximately 0.03ng/ml as a capture antibody.
Methanol-fixed Hela cells stained with 20 μg/ml mouse anti-B2MG mAb clone D6E4 and fluorescence labelled secondary antibody.
Staining of methanol-fixed Hela cells by Immunocytochemistry using Mouse anti-Human beta 2 Microglobulin monoclonal antibody (D2E9) at 1/50 (20 µg/ml).
Detect recombinant human B2MG antigen (100ng/lane) using anti-human B2MG mAb clone D2E9 at 1µg/ml dilution.
Fluorescence Flow Cytometry: Hela cells strained with Mouse anti-B2MG mAb clone D2E9 and fluorescence labelled secondary antibody. Black line histogram represents Hela cells stained with isotype control, Mouse anti-TB 38Kd antigen mAb (Cat.-No AM09229PU-N, B12F8) and fluorescence labelled secondary antibody.
Human Thymus (Formalin-Fixed, Paraffin-Embedded) stained with B2M antibody at 5 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blotting analysis ( non - reducing conditions ) of whole cell lysate of various cell lines using B2M monoclonal antibody, clone B2M - 01 ( Cat # MAB0899 ) . Lane 1 : Raji human Burkitt lymphoma cell line. Lane 2 : EL4 mouse lymphoblastic lymphoma cell line. Lane 3 : U 937 human histiocytic lymphoma cell line.
B2M MaxPab rabbit polyclonal antibody. Western Blot analysis of B2M expression in human placenta.
Western Blot analysis of B2M expression in transfected 293T cell line (H00000567-T01) by B2M MaxPab polyclonal antibody.Lane 1: B2M transfected lysate(13.70 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with B2M antibody (N-term) (Cat.#AP12414PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of B2M (arrow) using rabbit polyclonal B2M Antibody (N-term) (Cat#AP12414PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the B2M gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with B2M antibody (C-term) (Cat.#AP12415PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of B2M(arrow) using rabbit polyclonal B2M Antibody (C-term) (Cat.#AP12415PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the B2M gene (Lane 2) (Origene Technologies).
Immunofluorescent Staining: Chicken peripheral blood mononuclear cells were double-stained with Mouse anti-Chicken Beta-2-Microglobulin-FITC and Mouse anti-Chicken CD3-PE (Cat#AM08114FC-N). Small lymphocytes were then gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 µg/10e6 cells.
Immunohistochemistry analysis of Formalin-Fixed Paraffin-Embedded Human Liver tissue, using AM08319PU-N at 10 µg/ml.
Immunohistochemistry: AM08384PU-N B2M antibody staining of Formalin-Fixed Paraffin-Embedded of Human Liver at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Flow Cytometry: AM08384PU-N B2M antibody staining of Human peripheral blood lymphocytes followed by anti-Mouse FITC.
The membrane blot was probed with B2M monoclonal antibody ( Cat # MAB5428 ) ( 1µg/ml ).Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection system.Arrows indicate cellular recombinant B2M ( 12 kDa ).Lane 1. Marker.Lane 2. HeLa cell lysate ( induced ).Lane 3. MD231.Lane 4. U251.Lane 5. Purified B2M protein.
Detect recombinant human B2MG antigen (100ng/lane) using anti-human B2MG mAb D6D1 at 1μg/mL dilution.
Stainning of methanol-fixed Hela cells: Mouse anti-Human beta 2 Microglobulin monoclonal antibody (D2A10) 1/50 (20 µg/ml).
Detect recombinant human B2MG antigen (100ng/lane) using anti-human B2MG mAb D2A10 at 1 μg/ml dilution.
Above is the histogram of Mouse anti-Human beta 2 Micro Globulin monoclonal antibody (D2A10) 1:100 (10ug/mL) staining of Hela cells. Black line histogram represents the isotype control, Mouse anti-TB 38Kd antigen monoclonal antibody (Clone B12F8).
Staining of methanol-fixed Hela cells by Immunocytochemistry: Mouse anti-Human beta 2 Microglobulin monoclonal antibody (D3A2) 1:50 (20 µg/ml).
Detect recombinant human B2MG antigen (100ng/lane) using anti-human B2MG mAb D3A2 at 1 μg/ml.
Above is the histogram of Hela cells stained with anti-Human B2MG mAb D3A2 and fluorescence labelled secondary antibody. Black line represents histogram of Hela cells stained with isotype control, anti-TB 38Kd antigen mAb B12F8.
Human Thymus; WB Suggested Anti-B2M Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Thymus; B2M antibody - N-terminal region (ARP56555_P050) in Human Thymus cells using Western Blot
Recombinant human B2M, 21-119aa, His-tagged
Human Prostate (formalin-fixed, paraffin-embedded) stained with B2M antibody AM20523PU-N at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human β2-microglobulin (B2M-01).Lane 1: RAJI human Burkitt lymphoma cell lineLane 2: EL4 mouse lymphoblastic lymphoma cell lineLane 3: U937 human histiocytic lymphoma cell line
Surface staining of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) PE.
Surface staining of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) FITC.
COP5 transiently transfected with Beta-2-microglobulin cDNA stained by clone mAb4
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
B2M antibody Cat.-No AP31992PU-N staining of Human Duodenum lysate at 0.03 μg/ml (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-B2M Rabbit Polyclonal antibody. (TA590171)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-B2M Rabbit Polyclonal antibody. (TA590171)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-B2M Rabbit Polyclonal antibody. (TA590171)
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-B2M Rabbit Polyclonal antibody. (TA590171)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-B2M Rabbit Polyclonal antibody. (TA590171)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-B2M polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B2M (RC207587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-B2M.
Western Blot: beta 2 Microglobulin Antibody - Western Blot was performed using affinity purified SEQer B2M antibody, aa(21-119) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer B2M antibody, aa(21-119) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
ELISA: beta 2 Microglobulin Antibody
Standard curve for ELISA analysis with B2M recombinant protein (dilution range from 0.3ng/ml to 200ng/ml) using B2M Capture Antibody (Cat# TA803600/TA803617/TA802776) at 5µg/ml and HRP conjugated B2M Detection mAb (Cat# TA590171) at 0.125µg/ml.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-B2M mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802776)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-B2M mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802776)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B2M (RC207587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-B2M.
Standard curve for ELISA analysis with B2M recombinant protein (dilution range from 0.3ng/ml to 200ng/ml) using B2M Capture Antibody (Cat# TA803600/TA803617/TA802776) at 5µg/ml and HRP conjugated B2M Detection mAb (Cat# TA590171) at 0.125µg/ml.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-B2M mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803617)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B2M (RC207587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-B2M.
Standard curve for ELISA analysis with B2M recombinant protein (dilution range from 0.3ng/ml to 200ng/ml) using B2M Capture Antibody (Cat# TA803600/TA803617/TA802776) at 5µg/ml and HRP conjugated B2M Detection mAb (Cat# TA590171) at 0.125µg/ml.
(0.3ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-B2M antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-B2M antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-B2M antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC of Rabbit anti-Beta-2-Microglobulin Antibody. Tissue: normal human skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Left panel: isotype control, Right panel: ß2 microglobulin antibody at 1 ug/ml for 20 min at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ß2 microglobulin is cell membrane (and to lesser amount cytoplasmatic compartment).
IF of Rabbit Anti-Beta-2-microglobulin antibody. Tissue: Human Embryonic Kidney cells (HEK 293 cells). Primary antibody: ß-2 microglobulin antibody at 1:200 for overnight at 4°C. Secondary antibody: donkey anti-rabbit secondary antibody conjugated with Alexa Fluor 488 fluorochrome at 1:1,000 for 45 min at RT. Localization: ß2-microglobulin is mainly on the cell surface and occasionally cytoplasmic. Staining: Beta-2-microglobulin as green fluorescent signal.
IHC of Rabbit anti-Beta-2-Microglobulin Antibody. Tissue: normal human skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Left panel: isotype control, Right panel: ß2 microglobulin antibody at 1 ug/ml for 20 min at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ß2 microglobulin is cell membrane (and to lesser amount cytoplasmatic compartment).
IF of Rabbit Anti-Beta-2-microglobulin antibody. Tissue: Human Embryonic Kidney cells (HEK 293 cells). Primary antibody: ß-2 microglobulin antibody at 1:200 for overnight at 4°C. Secondary antibody: donkey anti-rabbit secondary antibody conjugated with Alexa Fluor 488 fluorochrome at 1:1,000 for 45 min at RT. Localization: ß2-microglobulin is mainly on the cell surface and occasionally cytoplasmic. Staining: Beta-2-microglobulin as green fluorescent signal.
Western blot analysis of B2M in SK-N-SH cell lysate with B2M antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of B2M in mouse brain tissue with B2M antibody 5 ug/mL.
Immunofluorescence of B2M in mouse brain tissue with B2M antibody at 20 ug/mL.
Predicted band size: 14 kDa . Positive control: Raji cell and human thyroid cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 15%SDS-PAGE Lane1: Raji cell lysate Lane2: Human thyroid cancer tissue lysate Lysate:s 40 ug per lane Primary antibody: 1/125 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)
Predicted band size: 14 kDa . Positive control: Human kidney tissue and Raji cell lysate. Recommended dilution: 1/200-1000. (Gel: 15%SDS-PAGE Lane1: Human kidney tissue lysate Lane2: Raji cell lysate Lysates: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds)
Predicted cell location: Cytoplasm and membrane. Positive control: Human lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using B2M antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification: x 200)
Western blot analysis of extracts of various cell lines, using B2M antibody.
Immunohistochemistry of paraffin-embedded human stomach cancer using B2M antibody at dilution of 1:200 (200x lens).
Immunohistochemistry of paraffin-embedded mouse heart using B2M antibody at dilution of 1:200 (200x lens).
Immunofluorescence analysis of MCF7 cell using B2M antibody. Blue: DAPI for nuclear staining
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP31992PU-N | Beta-2-microglobulin (65-79) antibody |
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Goat | Aff - Purified | Bov, Hu | E, WB |
0.1 mg /
$380.00
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| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Western blotting. |
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| TA311281 | Beta-2-microglobulin (N-term) antibody |
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| Goat Anti-beta-2-microglobulin (B2M) Antibody | |||||||
 |
Goat | Aff - Purified | Bov, Hu | E, WB |
0.1 mg /
$360.00
|
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| OriGene Technologies, Inc. | |||||||
| AP31732PU-N | Beta-2-microglobulin antibody |
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Goat | IgG | Aff - Purified | Hu | E, P |
0.25 mg /
$425.00
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| OriGene Technologies GmbH | |||||||