AM00683PU-N Bovine Corona Virus E2 protein antibody

Swiss Prot Num:

P25193

Immunogen:

Bovine corona virus. Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Bovine Corona Virus Spike glycoprotein is cleaved into S1 and S2 Spike proteins. The precursor is processed into S1 and S2 by host cell furin or furin-like protease to yield the mature S1 and S2 proteins. The cleavage site between S1 and S2 requires the optimal sequence [KR]-X-[KR]-R.

S1 attaches the virion to the cell membrane by binding to 9-O-acetylated sialic acid containing proteins, initiating the infection.

S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.

Purification:

>95% pure (SDS-PAGE). Protein G chromatography

Buffer System:

PBS, pH 7.4 containing 0.09% Sodium azide

State:

Liquid purified IgG
Purified

Specificity:

Recognizes bovine corona virus surface antigen (peplomer).