Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » CD209 / DC-SIGN
discontinued

DDX0209P-50 CD209 / DC-SIGN antibody

See related secondary antibodies

Search for all "CD209 / DC-SIGN"

View Datasheet

Quick Overview

Mouse anti Human CD209 / DC-SIGN 111H2.02

DDX0209P-50

More Views

  • DDX0209P-50
  • DDX0209P-50
  • DDX0209P-50

Product Description for CD209 / DC-SIGN

Mouse anti Human CD209 / DC-SIGN 111H2.02.
Presentation: Purified
Product is tested for Frozen Sections, Paraffin Sections, Flow Cytometry.

Properties for CD209 / DC-SIGN

Product Category Primary Antibodies
Quantity 50 µg
Synonyms C-type lectin domain family 4 member L, CLEC4L, DC-SIGN1, DCSIGN, DCSIGN1, Dendritic Cell Marker, Dendritic cell-specific ICAM-3-grabbing non-integrin 1
Presentation Purified
Reactivity Hu
Applications C, F, P
Clonality Monoclonal
Clone 111H2.02
Host Mouse
Isotype IgG2b
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9NNX6
Immunogen:
HeLa cells stably transfected-with human DC-SIGN.
GeneID:
30835
Isotype control AM03110PU-N, SM12P
Application DDX0209P-50 / DDX0209P-100 purified: Surface flow cytometry, IHC Formol- paraffin, Bouin paraffin, Histowax.
DDX0209A488-50 / DDX0209A488-100 Alexa-Fluor®488: Surface Flow cytometry.
DDX0209A546-50 / DDX0209A546-100 Alexa-Fluor®647: IF. 
DDX0209A647-50 / DDX0209A647-100 Alexa-Fluor®647: Surface Flow cytometry. 
DDX0209B-50 DDX0209B-100 Biotin (on request): Surface Flow cytometry, IHC. 
Usage recommendation:
*This monoclonal antibody may be used between 5-20 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Background DC-SIGN (“DC Specific, ICAM-3 Grabbing, Nonintegrin”)/CD209 and liver/lymph node-specific ICAM-3-grabbing nonintegrin (L-SIGN) (CD299/DC-SIGNR for DC-SIGN-related molecule; DC-SIGN2) are closely related genes that map to chromosome 19p13.3.Both genes encode a member of the C-type lectin family of type II transmembrane proteins. The two receptors are 77% identical at the amino acid level, have similar ligands. They are expressed in different tissues. Both receptors have been shown to interact with ICAM-3 DC-SIGN is a high affinity receptor for HIV gp120 (Soilleux EJ. 2003, Clinical Science 104, 437-; Dakappagari N., et al. 2006, The J Immunol, 176, 426; Geijtenbeeck T.B., et al. 2000, Cell, 100, 57 ; .Bashirova A. et al., 2001, J.Exp. Med., 193, 671). The clone 111H2.02 anti-DC-SIGN blocks HIVgp120-DC-SIGN interaction and reacts on formalin-fixed tissues (Canard B et al, Immunol Lett. 2011, 135(1-2):165-72).
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Bashirova AA, Geijtenbeek TB, van Duijnhoven GC, van Vliet SJ, Eilering JB, Martin MP, et al. A dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN)-related protein is highly expressed on human liver sinusoidal endothelial cells and promotes HIV-1 infection. J Exp Med. 2001 Mar 19;193(6):671-8. PubMed PMID: 11257134. (Free PMC Article available, 5 images available)
  2. Soilleux EJ. DC-SIGN (dendritic cell-specific ICAM-grabbing non-integrin) and DC-SIGN-related (DC-SIGNR): friend or foe? Clin Sci (Lond). 2003 Apr;104(4):437-46. PubMed PMID: 12653690.
  3. Dakappagari N, Maruyama T, Renshaw M, Tacken P, Figdor C, Torensma R, et al. Internalizing antibodies to the C-type lectins, L-SIGN and DC-SIGN, inhibit viral glycoprotein binding and deliver antigen to human dendritic cells for the induction of T cell responses. J Immunol. 2006 Jan 1;176(1):426-40. PubMed PMID: 16365436.
  4. Canard B, Vachon H, Fontaine T, Pin JJ, Paul S, Genin C, et al. Generation of anti-DC-SIGN monoclonal antibodies capable of blocking HIV-1 gp120 binding and reactive on formalin-fixed tissue. Immunol Lett. 2011 Mar 30;135(1-2):165-72. doi: 10.1016/j.imlet.2010.11.002. Epub 2010 Nov 13. PubMed PMID: 21078343.
General Readings
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Buffer System:
Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol.
State:
Purified
Specificity
Specificity:
Human DC-SIGN.
Species cross-reactivity: positive staining on NIH3T3-transfected LSIGN.

Accessory Products

Recommended Secondary Antibodies for CD209 / DC-SIGN (11 products)

Catalog No. Host Clone/Iso. Pres.   React. Applications  

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit AP C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Biotin C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit FITC E, ICC/IF
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit HRP C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Texas Red ICC/IF
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Rabbit PE F
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Goat PE
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Donkey PE
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy2
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy3
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy5
    OriGene Technologies GmbH
Click here to see all secondary antibodies for CD209 / DC-SIGN
Compare Products

You have no items to compare.

My Cart

You have no items in your shopping cart.

Service
 
© Copyright 1998 - 2018 OriGene Technologies, Inc.
  • LinkedIn