discontinued

DDX0463P-50 SEMA6A antibody

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Quick Overview

Mouse anti Human SEMA6A 118E7

Product Description for SEMA6A

Mouse anti Human SEMA6A 118E7.
Presentation: Purified
Product is tested for Flow Cytometry.

Properties for SEMA6A

Product Category Primary Antibodies
Quantity 50 µg
Synonyms KIAA1368, SEMA6A-1, SEMAQ, Sema VIA, Semaphorin VIA, Semaphorin-6A, Semaphorin-6A-1
Presentation Purified
Reactivity Hu
Applications F
Clonality Monoclonal
Clone 118E7
Host Mouse
Isotype IgG1
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9H2E6
Immunogen:
human recombinant SEMA-6A
GeneID:
57556
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application DDX0463P-50 / DDX0463P-100 Purified: Surface Flow Cytometry.
DDX0463A488-50 / DDX0463A488-100 Alexa-fluor®488: Surface Flow Cytometry. 
DDX0463A546-50 / DDX0463A546-100 Alexa-fluor®546: IF.
DDX0463A647-50 / DDX0463A647-100 Alexa- fluor®647: (on request) IHC, Flow cytometry. 

Usage recommendation:
*This monoclonal antibody may be used between 5-20 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before using.
Background Semaphorins represent a large family composed of 19 secreted, GPI-linked or transmembrane members divided into 5 classes (3 to 7) and characterized by the presence of a 500 amino acid Sema domain in the Nterminus. Initially described for their role in axon guidance and apoptosis during neuronal development, some semaphorins like the class 4 regulate immune functions and are involved in organogenesis and angiogenesis. The transmembrane class 6 semaphorins include 4 members (SEMA-6A to 6D). SEMA-6A features an intracellular binding site for regulatory Evl protein. In the immune system, SEMA6A protein is restricted in vitro and in vivo to an activation status of Langerhans cells. (Gautier G et al., 2006 Am J Pathol., 168: 453-65).
Concentration 0.5 mg/ml
General Readings
  1. Gautier G, de Saint-Vis B, Sénéchal B, Pin JJ, Bates EE, Caux C, et al. The class 6 semaphorin SEMA6A is induced by interferon-gamma and defines an activation status of langerhans cells observed in pathological situations. Am J Pathol. 2006 Feb;168(2):453-65. PubMed PMID: 16436660. (Free PMC Article available, 8 images available)
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Purification:
QMA Hyper D ion exchange chromatography
Buffer System:
Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
State:
Purified
Specificity
Specificity:
Human SEMA-6A (epitope in extracellular domain).
Gene ID 57556

Accessory Products

Proteins and/or Positive Controls

Positive controls for SEMA6A (1 products)

Catalog No. Species Pres. Purity   Source  

SEMA6A overexpression lysate

SEMA6A overexpression lysate
0.1 mg / $495.00
  OriGene Technologies, Inc.
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