Product Description for TGFB1
Mouse anti Human TGFB1 8C4.
Presentation: Azide Free
Product is tested for Frozen Sections, Enzyme Immunoassay, Functional assay.
Presentation: Azide Free
Product is tested for Frozen Sections, Enzyme Immunoassay, Functional assay.
Properties for TGFB1
| Product Category | Primary Antibodies |
|---|---|
| Target Category | |
| Quantity | 0.5 mg |
| Synonyms | TGF-beta-1, TGFB, Transforming growth factor beta-1 |
| Presentation | Azide Free |
| Reactivity | Hu |
| Applications | C, E, FN |
| Clonality | Monoclonal |
| Clone | 8C4 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Application | ELISA. Neutralizing Activity: This MAb neutralizes TGF beta activity in vitro in inhibition assay of CCL/64 cell growth and also neutralize the growth promoting action of TGF-beta in the NRK-49F colony forming assays. The effect of micro-injection of this MAb into one blastomere of two cell stage Xenopus embryos indicated that this MAb was able to neutralize effectively the bioactivity of TG beta in vivo. Immunohistochemistry: May be used in immunohistochemical techniques to locate TGF-beta within tissues. Western Blotting：This antibody can detect 40ng/lane of human TGF-beta on Western Blot. The blot below was stained with HRP substrate 4-Chloro-1-naphthol and H2O2. |
| Background | TGF beta 1 (Transforming Growth Factor Beta 1) is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed as well as non neoplastic tissues, release transforming growth factors; and essentially all cells possess a specific TGF beta 1 receptor. The multimodal nature of TGF beta 1 is seen in its ability to stimulate or inhibit cellular proliferation. TGF beta 1 plays a fundamental role in tissue growth and differentiation involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function. The C-terminal 112 amino acids constitute the mature protein. |
| Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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| Gene ID | 7040 |
Accessory Products
Proteins and/or Positive Controls
Proteins for TGFB1 (11 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| PA161 |
TGFB1 |
||||||
|
Human | Purified | > 98 % pure by SDS-PAGE gel and HPLC analyses | CHO cells | |||
| OriGene Technologies GmbH | |||||||
| TP300973 |
TGFB1 |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP720760 |
TGFB1 |
||||||
|
|
Human |
> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
10 µg /
$299.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP723438 |
TGFB1 |
||||||
|
Human |
> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
10 µg /
$230.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP723439 |
TGFB1 |
||||||
|
Human |
> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
CHO cells |
10 µg /
$230.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP723781 |
TGFB1 |
||||||
|
Human |
> 98 % Preparation: . Purity Detail: >98%, as determined by Coomassie stained SDS-PAGE. |
CHO cells |
10 µg /
$355.00
|
|||
| OriGene Technologies, Inc. | |||||||
| AR09266PU-L |
TGFB1 (279-390) |
||||||
|
Human | Purified | > 95 % by SDS - PAGE | E. coli |
0.5 mg /
$1,570.00
|
||
| OriGene Technologies GmbH | |||||||
| AR09266PU-N |
TGFB1 (279-390) |
||||||
|
|
Human | Purified | > 95 % by SDS - PAGE | E. coli |
0.1 mg /
$570.00
|
||
| OriGene Technologies GmbH | |||||||
| PA161A |
TGFB1 |
||||||
|
|
Human | Purified | > 98 % pure by SDS-PAGE gel and HPLC analyses | CHO cells | |||
| OriGene Technologies GmbH | |||||||
| PA523 |
TGFB1 |
||||||
|
|
Human | Purified | > 98 % pure by SDS-PAGE gel and HPLC analyses | HEK293 cells | |||
| OriGene Technologies GmbH | |||||||
| PA523X |
TGFB1 |
||||||
|
|
Human | Purified | > 98 % pure by SDS-PAGE gel and HPLC analyses | HEK293 cells | |||
| OriGene Technologies GmbH | |||||||
Positive controls for TGFB1 (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| LY424586 |
TGFB1 overexpression lysate |
||||||
|
|||||||
| OriGene Technologies, Inc. | |||||||
Recommended Secondary Antibodies for TGFB1 (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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