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SM1376P Complement factor I antibody

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0.25 mg / $380.00
Delivery Time: 5-7 working days*
(*) valid for US customers only

View Datasheet

Quick Overview

Mouse anti Human Complement factor I OX-21

SM1376P

Product Description for Complement factor I

Mouse anti Human Complement factor I OX-21.
Presentation: Purified
Product is tested for Paraffin Sections, Enzyme Immunoassay, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot, Flow Cytometry.

Properties for Complement factor I

Product Category Primary Antibodies
Target Category
Quantity 0.25 mg
Synonyms AHUS3, C3B/C4B inactivator, C3BINA, C3b-INA, CFI, IF, KAF
Presentation Purified
Reactivity Hu
Applications E, F, IP, P, R, WB
Clonality Monoclonal
Clone OX-21
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P05156
Immunogen:
Purified Human Complement Factor 1.  
Spleen cells from immunised FI cross DBA/2 x Balb/c mice were fused with cells of the NS-1 mouse myeloma cell line.
GeneID:
3426
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA. 
Titre: 1/40 000 (plates coated with 2 µg/ml purified Factor I and HRPO Goat anti-Mouse IgG secondary antibody).
Western Blot.  
Flow Cytometry.
Immunoprecipitation.
Immunohistochemistry on Paraffin Sections (20 µg/ml).
Background Factor I is a plasma protein of molecular weight 88 kDa, consisting of two disulphide linked chains of 50 and 38 kDa. Factor I is a serine protease with a high degree of specificity for C3b and C4b. It has a requirement for protein cofactors for cleavage of these complement proteins, Factor H, CR1 or MCP are required for C3b cleavage and C4bp or CR1 for C4b cleavage.
Concentration 1.0 mg/ml.
General Readings
  1. Hsiung L, Barclay AN, Brandon MR, Sim E, Porter RR. Purification of human C3b inactivator by monoclonal-antibody affinity chromatography. Biochem J. 1982 Apr 1;203(1):293-8. PubMed PMID: 7103942. (Free PMC Article available)
  2. Kavanagh D, Kemp EJ, Mayland E, Winney RJ, Duffield JS, Warwick G, et al. Mutations in complement factor I predispose to development of atypical hemolytic uremic syndrome. J Am Soc Nephrol. 2005 Jul;16(7):2150-5. Epub 2005 May 25. PubMed PMID: 15917334.
  3. Kraus E, James W, Barclay AN. Cutting edge: novel RNA ligands able to bind CD4 antigen and inhibit CD4+ T lymphocyte function. J Immunol. 1998 Jun 1;160(11):5209-12. PubMed PMID: 9605115.
  4. Sedgwick JD, Ford AL, Foulcher E, Airriess R. Central nervous system microglial cell activation and proliferation follows direct interaction with tissue-infiltrating T cell blasts. J Immunol. 1998 Jun 1;160(11):5320-30. PubMed PMID: 9605131.
  5. Benedict Seddon and Don Mason, 1999. Regulatory T cells in the control of autoimmunity: The essential role of transforming growth factor β and interleukin 4 in the prevention of autoimmune thyroiditis in rats by peripheral CD4+ CD45RC- cells and CD4+ CD8- thymocytes. J. Exp. Med. Vol. 189, Number 2:279-288.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein G Chromatography
Buffer System:
PBS
Preservatives:
0.02% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
This monoclonal antibody clone OX-21 recognizes Human Complement Protein Factor I.
Factor I has two polypeptide chains, 56kD and 38kD. This antibody detects only the 56kD chain. 
This clone has been reported not to cross react with Rat.
Species:
Human.

Accessory Products

Proteins and/or Positive Controls

Proteins for Complement factor I (1 products)

Catalog No. Species Pres. Purity   Source  

Complement factor I
Complement factor I Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

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