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AM01335PU-N Complement factor I antibody

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0.1 mg / $730.00
Delivery Time: 3-5 working days*
(*) valid for US customers only

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Quick Overview

Mouse anti Human Complement factor I 3R/8

AM01335PU-N

Product Description for Complement factor I

Mouse anti Human Complement factor I 3R/8.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Functional assay, Western blot / Immunoblot.

Properties for Complement factor I

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms AHUS3, C3B/C4B inactivator, C3BINA, C3b-INA, CFI, IF, KAF
Presentation Purified
Reactivity Hu
Applications C, E, FN, WB
Clonality Monoclonal
Clone 3R/8
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P05156
Immunogen:
Native human factor I from serum.
GeneID:
3426
Isotype control AM03095PU-N, SM10P (for use in human samples)
Add. information This antibody blocks function of complement factor I. Removal of Sodium Azide is recommended prior to use in functional assays.
Application ELISA: 1/3000 - 1/4000.
Western Blot.
Functional Assays.
Immunohistochemistry on frozen sections.
Recommended Positive Control: Kidney from post streptoccal glomerulonephritis patients.
Background Factor I is an 88 kDa protein consisting of one polypeptide chain of 35.4 kDa and one of 27.6 kDa. Factor I is a serine protease which inactivates complement components C3b and C4b. C3b cleavage requires the presence of cofactors complement component (3b/4b) receptor 1 (CR1), membrane cofactor protein or factor H, while C4b cleavage requires complement component 4 binding protein or CR1. Factor I has no natural inhibitors. Dysfunctional factor I can lead to uncontrolled activation of the alternative complement pathway causing in systemic depletion of C3 and lower levels of factors B and H, resulting in recurrent pyogenic infections. Defects in CFI are also associated with haemolytic uraemic syndrome.
Concentration 1.0mg/ml
General Readings
  1. Dragon-Durey MA, Frémeaux-Bacchi V. Atypical haemolytic uraemic syndrome and mutations in complement regulator genes. Springer Semin Immunopathol. 2005 Nov;27(3):359-74. Epub 2005 Nov 11. PubMed PMID: 16189652.
  2. Nilsson SC, Karpman D, Vaziri-Sani F, Kristoffersson AC, Salomon R, Provot F, et al. A mutation in factor I that is associated with atypical hemolytic uremic syndrome does not affect the function of factor I in complement regulation. Mol Immunol. 2007 Mar;44(8):1835-44. Epub 2006 Nov 7. PubMed PMID: 17084897.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography on Protein A
Buffer System:
Borate buffered saline pH 8.4 containing 0.02% Sodium Azide
State:
Liquid purified Ig
Purified
Specificity
Specificity:
This antibody recognises complement factor I, a member of the peptidase S1 family present in the blood plasma.
Species:
Human.
Gene ID 3426

Accessory Products

Proteins and/or Positive Controls

Proteins for Complement factor I (1 products)

Catalog No. Species Pres. Purity   Source  

Complement factor I
Complement factor I Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

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Click here to see all secondary antibodies for Complement factor I
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