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discontinued

AM10073PU-N CD8 (C-term) antibody

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OriGene recommends this product for Immunohistochemistry.

Quick Overview

Mouse anti Human CD8 C8/144B

AM10073PU-N

Product Description for CD8

Mouse anti Human CD8 C8/144B.
Properties: (C-term)
Presentation: Purified
Product is tested for Western blot / Immunoblot, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Flow Cytometry, Frozen Sections, Paraffin Sections.

Properties for CD8

Product Category Primary Antibodies
Target Category
Quantity 0.2 mg
Synonyms CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Presentation Purified
Reactivity Hu
Applications C, F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone C8/144B
Host Mouse
Isotype IgG1
Molecular weight 32 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P01732
Immunogen:
A 13 amino acid peptide from C-terminal cytoplasmic domain of alpha chain of Human CD8 molecule.
GeneID:
925
Property C-term
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA: Use BSA free Antibody for coating.
Flow Cytometry: 0.5-1 µg/106 cells. 
Immunofluorescence: 0.5-1 µg/ml.
Western Blot:  0.5-1 µg/ml.
Immunoprecipitation: 1-2 µg/500 µg protein lysate. 
Immunohistochemistry on Frozen Sections: 1-2 µg/ml.
Immunohistochemistry on Paraffin Sections: 1-2 µg/ml.
Incubation Time: 30 minutes at RT. 
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Positive Control: HuT78 or hPBL,Tonsil.
Background CD8 is a cell surface glycoprotein molecule expressed on the surface of a subset of T cells, generally the cytotoxic/suppressor cells, as well as on some natural killer cells. There are two forms of CD8, either as an alpha-alpha homodimer or alpha-beta heterodimer. The pathological role of CD8 has been observed in familial CD8 deficiency. 
A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.
Concentration 0.2 mg/ml
General Readings
  1. Mason DY, Cordell JL, Gaulard P, Tse AG, Brown MH. Immunohistological detection of human cytotoxic/suppressor T cells using antibodies to a CD8 peptide sequence. J Clin Pathol. 1992 Dec;45(12):1084-8. PubMed PMID: 1479035. (Free PMC Article available)
Storage Store undiluted at 2-8°C. 
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Specificity
Specificity:
Recognizes Human CD8. Other species not tested. 
Cellular Localization: Cell surface.

Accessory Products

Proteins and/or Positive Controls

Proteins for CD8 (1 products)

Catalog No. Species Pres. Purity   Source  

CD8 (full length, N-term HIS tag, transcript variant 2)
CD8 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		E. coli
50 µg / $205.00
  OriGene Technologies, Inc.

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