AM50194PU-S CD44 antibody

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0.1 mg / $430.00
Delivery Time: 1-3 working days*
(*) valid for US customers only

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Mouse anti Human, Monkey, Baboon CD44 SPM544

AM50194PU-S

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  • AM50194PU-S

Product Description for CD44

Mouse anti Human, Monkey, Baboon CD44 SPM544.
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot.

Properties for CD44

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms CDw44, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein 1
Presentation Purified
Reactivity Bab, Hu, Mky
Applications C, F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone SPM544
Host Mouse
Isotype IgG2a
Molecular weight 80-95kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P16070
Immunogen:
Stimulated human leukocytes.
GeneID:
960
Isotype control AM03096PU-N
Application ELISA: Use BSA free Antibody for coating. 
Flow Cytometry: 0.5-1 µg/million cells.
Immunofluorescence: 0.5-1 µg/ml. 
Western Blotting: 0.5-1 µg/ml.
Immunoprecipitation: 0.5-1 µg/500 µg protein lysate. 
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µ/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: HeLa cells or paracortex in tonsil or lymph node.
Background The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
Concentration 0.2 mg/ml
General Readings
  1. Schlossman SF, et. al. Leucocyte Typing V, p1713-1719, Oxford Univ. Press, 1995.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Specificity
Specificity:
Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes. Its epitope is resistant to digestion by trypsin and chymotrypsin.
Cellular Localization: Cell surface.
Species:
Human, Baboon, and Green Monkey.

Accessory Products

Proteins and/or Positive Controls

Proteins for CD44 (4 products)

Catalog No. Species Pres. Purity   Source  

CD44 (transcript variant 2)

CD44 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

CD44 (transcript variant 1)

CD44 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

CD44 (transcript variant 1)

CD44 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / $299.00
  OriGene Technologies, Inc.

CD44 (transcript variant 2)

CD44 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
  OriGene Technologies, Inc.

Positive controls for CD44 (3 products)

Catalog No. Species Pres. Purity   Source  

CD44 overexpression lysate

CD44 overexpression lysate
0.1 mg / $295.00
  OriGene Technologies, Inc.

CD44 overexpression lysate

CD44 overexpression lysate
0.1 mg / $295.00
  OriGene Technologies, Inc.

CD44 overexpression lysate

CD44 overexpression lysate
0.1 mg / $495.00
  OriGene Technologies, Inc.
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