Product Description for CD3
Mouse anti Human CD3 AT3H3.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.
Properties for CD3
| Product Category | Primary Antibodies |
|---|---|
| Target Category | |
| Quantity | 0.1 ml |
| Synonyms | T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4 |
| Presentation | Purified |
| Reactivity | Hu |
| Applications | E, WB |
| Clonality | Monoclonal |
| Clone | AT3H3 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Isotype control | AM03095PU-N, SM10P (for use in human samples) |
| Application | The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000. |
| Background | The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multi subunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. |
| Concentration | 1.0 mg/ml |
| General Readings | Gold DP., et al. (1986) Nature 321(6068): 431-434Soudais C., et al. (1993) Nat Genet 3(1):77-81. |
| Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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Accessory Products
Proteins and/or Positive Controls
Proteins for CD3 (5 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP308276 |
CD3 |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP700034 |
CD3 |
||||||
|
Human |
> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP720690 |
CD3 |
||||||
|
Human |
> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
10 µg /
$299.00
|
|||
| OriGene Technologies, Inc. | |||||||
| AR50677PU-N |
CD3 (23-126, His-tag) |
||||||
|
Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.25 mg /
$820.00
|
||
| OriGene Technologies GmbH | |||||||
| AR50677PU-S |
CD3 (23-126, His-tag) |
||||||
|
|
Human | Purified | > 90 % by SDS - PAGE | E. coli |
50 µg /
$320.00
|
||
| OriGene Technologies GmbH | |||||||
Positive controls for CD3 (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| LY400242 |
CD3E overexpression lysate |
||||||
|
0.1 mg /
$295.00
|
||||||
| OriGene Technologies, Inc. | |||||||
Recommended Secondary Antibodies for CD3 (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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