VWA5A antibody
Principal name
VWA5A antibody
Alternative names for VWA5A antibody
VWA5A, BCSC1, LOH11CR2A, Breast cancer suppressor candidate 1, BCSC-1, von Willebrand factor A domain-containing protein 5A, Loss of heterozygosity 11 chromosomal region 2 gene A protein
Ncbi ID
9606, NP_001123614.1, NM_014622, NM_172767, NP_938057, NM_198315, NP_055437, NP_001123614, BC004727, NM_001145957, NM_198755, NM_001130142
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P)
Background of VWA5A antibody
- Kit Component:
- - KN201474G1, VWA5A gRNA vector 1 in pCas-Guide vector
- KN201474G2, VWA5A gRNA vector 2 in pCas-Guide vector
- KN201474D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
LOH11CR2A MaxPab polyclonal antibody. Western Blot analysis of LOH11CR2A expression in human stomach.
LOH11CR2A MaxPab polyclonal antibody. Western Blot analysis of LOH11CR2A expression in human liver.
Western Blot analysis of VWA5A expression in transfected 293T cell line (H00004013-T01) by VWA5A MaxPab polyclonal antibody.Lane 1: LOH11CR2A transfected lysate(86.46 KDa).Lane 2: Non-transfected lysate.
LOH11CR2A MaxPab polyclonal antibody. Western Blot analysis of LOH11CR2A expression in human stomach.
LOH11CR2A MaxPab polyclonal antibody. Western Blot analysis of LOH11CR2A expression in human liver.
Western Blot analysis of VWA5A expression in transfected 293T cell line (H00004013-T01) by VWA5A MaxPab polyclonal antibody.Lane 1: LOH11CR2A transfected lysate(86.46 KDa).Lane 2: Non-transfected lysate.
Human Jurkat; WB Suggested Anti-LOH11CR2A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate; LOH11CR2A antibody - N-terminal region (ARP55093_P050) in Human Jurkat cells using Western Blot
Mouse Kidney; WB Suggested Anti-Vwa5a Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Kidney; Vwa5a antibody - C-terminal region (ARP55094_P050) in Mouse Kidney cells using Western Blot
Immunofluorescent analysis of VWA5A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of VWA5A expression in HeLa (A); SVT2 (B) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-VWA5A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501569)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-VWA5A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501569)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-VWA5A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501569)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VWA5A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501569)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-VWA5A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501569)
Anti-VWA5A mouse monoclonal antibody (TA501569) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VWA5A(RC212185).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VWA5A (RC212185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VWA5A.
Flow cytometric Analysis of Hela cells, using anti-VWA5A antibody(TA501569),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-VWA5A antibody(TA501569),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Anti-VWA5A mouse monoclonal antibody (TA501570) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VWA5A(RC212185).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VWA5A (RC212185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VWA5A.
Anti-VWA5A mouse monoclonal antibody (TA501571) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VWA5A(RC212185).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VWA5A (RC212185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VWA5A.
HEK293T cells transfected with either RC212185 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-VWA5A antibody(TA501571), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-VWA5A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501572)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-VWA5A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501572)
Anti-VWA5A mouse monoclonal antibody (TA501572) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VWA5A(RC212185).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VWA5A (RC212185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VWA5A.
Anti-VMA5A mouse monoclonal antibody (TA501596) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VMA5A(RC212185).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VMA5A (RC212185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VMA5A.
HEK293T cells transfected with either RC212185 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-VMA5A antibody(TA501596), and then analyzed by flow cytometry.
Anti-VWA5A mouse monoclonal antibody (TA501597) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VWA5A(RC212185).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VWA5A (RC212185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VWA5A.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-VWA5A monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC212185 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-VWA5A antibody(TA501597), and then analyzed by flow cytometry.
Anti-VWA5A mouse monoclonal antibody (TA501636) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VWA5A(RC212185).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VWA5A (RC212185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VWA5A.
HEK293T cells transfected with either RC212185 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-VWA5A antibody(TA501636), and then analyzed by flow cytometry.
Anti-VWA5A mouse monoclonal antibody (TA501637) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VWA5A(RC212185).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VWA5A (RC212185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VWA5A.
HEK293T cells transfected with either RC212185 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-VWA5A antibody(TA501637), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-VWA5A antibody(TA501637),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Anti-VMA5A mouse monoclonal antibody (TA501638) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VMA5A(RC212185).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VMA5A (RC212185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VMA5A.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VWA5A (RC212185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VWA5A.
HEK293T cells transfected with either RC212185 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-VWA5A antibody(TA501644), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-VWA5A antibody(TA501644),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Anti-VWA5A mouse monoclonal antibody (TA501654) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VWA5A(RC212185).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VWA5A (RC212185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VWA5A.
HEK293T cells transfected with either RC212185 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-VWA5A antibody(TA501654), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-VWA5A antibody(TA501654),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Hela cells, using anti-VWA5A antibody(TA501654),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Western blot analysis of extracts from 293 cells, using LHR2A antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis on 293 cell lysate using VWA5A Antibody
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Human Adrenal pheochromocytoma tissue, Jurkat cells, A549 cells, Primary antibody: (VWA5A Antibody) at dilution 1/275, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using (VWA5A Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (VWA5A Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA312370 | VWA5A antibody |
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| Rabbit polyclonal anti-LHR2A antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
$335.00
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| OriGene Technologies, Inc. | |||||||
| TA347516 | VWA5A antibody |
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| Rabbit Polyclonal Anti-VWA5A Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.1 mg /
$335.00
|
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| OriGene Technologies, Inc. | |||||||
| TA349707 | VWA5A antibody |
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| Rabbit Polyclonal Anti-VWA5A Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P, WB |
0.1 ml /
$335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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