USP10 antibody
Principal name
USP10 antibody
Alternative names for USP10 antibody
KIAA0190, Ubiquitin carboxyl-terminal hydrolase 10, Deubiquitinating enzyme 10, Ubiquitin thioesterase 10, Ubiquitin-specific-processing protease 10
SwissProt ID
A5PJS6 (Bovin), P52479 (Mouse), Q14694 (Human), Q3KR59 (Rat), Q5ZJN4 (Chick), Q6DIJ4 (Xentr)
Ncbi ID
9606, NP_005144.2, NP_005144, NM_001034146, AAH00263.1, NP_033488.1, NM_009462, NM_005153
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)
Background of USP10 antibody
- Kit Component:
- - KN318765G1, Usp10 gRNA vector 1 in pCas-Guide vector
- KN318765G2, Usp10 gRNA vector 2 in pCas-Guide vector
- KN318765D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Detection limit for recombinant GST tagged USP10 is approximately 0.1ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with USP10 polyclonal antibody ( Cat # PAB4190 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
The USP10 polyclonal antibody ( Cat # PAB4190 ) is used in Western blot to detect USP10 in A-375 cell lysate .
Immunofluorescence of purified MaxPab antibody to USP10 on HeLa cell. [antibody concentration 10 ug/ml]
USP10 MaxPab polyclonal antibody. Western Blot analysis of USP10 expression in human placenta.
Western Blot analysis of USP10 expression in transfected 293T cell line (H00009100-T01) by USP10 MaxPab polyclonal antibody.Lane 1: USP10 transfected lysate(87.78 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of USP10 expression in transfected 293T cell line (H00009100-T01) by USP10 MaxPab polyclonal antibody.Lane 1: USP10 transfected lysate(87.20 KDa).Lane 2: Non-transfected lysate.
USP10 MaxPab rabbit polyclonal antibody. Western Blot analysis of USP10 expression in Hela NE.
Human THP-1; WB Suggested Anti-USP10 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: THP-1 cell lysate; USP10 antibody - middle region (ARP59302_P050) in Human THP-1 cells using Western Blot
Rat Lung; WB Suggested Anti-Usp10 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Rat Lung; Usp10 antibody - C-terminal region (ARP59301_P050) in Rat Lung cells using Western Blot
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503878)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503878)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503878)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503878)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503878)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503878)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503878)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503878)
Anti-USP10 mouse monoclonal antibody (TA503878) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY USP10(RC200835).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP10 (RC200835, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP10.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-USP10 monoclonal antibody.
Flow cytometric Analysis of Hela cells, using anti-USP10 antibody(TA503878),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504007)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504007)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504007)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504007)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504007)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-USP10 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP10 (RC200835, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP10.
Flow cytometric Analysis of Jurkat cells, using anti-USP10 antibody(TA504007),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504130)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504130)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504130)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504130)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504130)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504130)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504130)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP10 (RC200835, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP10.
Flow cytometric Analysis of Hela cells, using anti-USP10 antibody(TA504130),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-USP10 antibody(TA504130),(Red), compared to a nonspecific negative control antibody,(Blue).
Western blot analysis of USP10 in Jurkat cell lysate with USP10 antibody at (A) 1 and (B) 2 ug/mL.
Immunofluorescence of USP10 in Jurkat cells with USP10 antibody at 20 ug/mL.
Immunocytochemistry of USP10 in Jurkat cells with USP10 antibody at 20 ug/mL.
WB Suggested Anti-USP10 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: THP-1 cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA319768 | USP10 (N-term) antibody |
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| Rabbit Polyclonal USP10 Antibody | |||||||
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Rabbit | Aff - Purified | Hu, Ms | E, ICC/IF, WB |
0.1 mg /
$415.00
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| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| AP31186PU-N | USP10 (N-term) antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, P, WB |
50 µg /
$425.00
|
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| OriGene Technologies GmbH | |||||||
| +2 additional images |
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