TP53RK antibody
Principal name
TP53RK antibody
Alternative names for TP53RK antibody
TP53-regulating kinase, p53-related protein kinase, PRPK, C20orf64, Nori-2
Ncbi ID
9606, 5874, NP_291028.3, NP_291028, NP_076304.2, NM_023815, NM_033550
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of TP53RK antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
Immunoperoxidase of monoclonal antibody to TP53RK on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 2ug/ml]
Western Blot analysis of TP53RK expression in transfected 293T cell line by TP53RK monoclonal antibody (M01), clone 4B9-1H3.Lane 1: TP53RK transfected lysate(28 KDa).Lane 2: Non-transfected lysate.
TP53RK monoclonal antibody (M01), clone 4B9-1H3 Western Blot analysis of TP53RK expression in Jurkat ( Cat # L017V1 ).
Detection limit for recombinant GST tagged TP53RK is approximately 1ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to TP53RK on HeLa cell. [antibody concentration 10 ug/ml]
TP53RK monoclonal antibody (M02), clone 2A9 Western Blot analysis of TP53RK expression in COLO 320 HSR ( Cat # L020V1 ).
Detection limit for recombinant GST tagged TP53RK is approximately 0.03ng/ml as a capture antibody.
Detection limit for recombinant GST tagged TP53RK is approximately 0.1ng/ml as a capture antibody.
Detection limit for recombinant GST tagged TP53RK is approximately 0.3ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to TP53RK on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 3 ug/ml]
Detection limit for recombinant GST tagged TP53RK is approximately 1ng/ml as a capture antibody.
Detection limit for recombinant GST tagged TP53RK is approximately 0.1ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with TP53RK polyclonal antibody ( Cat # PAB3315 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
The TP53RK polyclonal antibody ( Cat # PAB3315 ) is used in Western blot to detect TP53Rk in mouse thymus tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
The anti-PRPK Pab is used in Western blot to detect PRPK in mouse thymus tissue lysate.
Rat thyrocytes-FRTL-5; . Sample Type :. Rat thyrocytes-FRTL-5 . Primary Antibody Dilution :. 1:100 . Secondary Antibody :. Anti-rabbit-FITC . Secondary Antibody Dilution :. 1:100 . Color/Signal Descriptions :. Green: TP53RKBlue: DAPI. Gene Name :. TP53RK. Submitted by :. Syed A Morshed, Mount Sinai School of Medicine and James J Peters VA Medical Center; TP53RK antibody - N-terminal region (ARP52434_P050) in Rat thyrocytes-FRTL-5 cells using Immunofluorescence
Human HT1080; WB Suggested Anti-TP53RK Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HT1080 cell lysate; TP53RK antibody - N-terminal region (ARP52434_P050) in Human HT1080 cells using Western Blot
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
TP53RK monoclonal antibody, clone 4B9-1H3 Western Blot analysis of TP53RK expression in Jurkat.
Detection limit for recombinant GST tagged TP53RK is approximately 1 ng/ml as a capture antibody.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TP53RK mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA808226)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53RK (RC209748, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53RK.(1:2000)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TP53RK mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808230)(1:150)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TP53RK mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808230)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53RK (RC209748, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53RK.(1:2000)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-TP53RK mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA808231)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TP53RK mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA808231)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53RK (RC209748, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53RK.(1:2000)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TP53RK mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA808232)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TP53RK mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA808232)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TP53RK mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA808232)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53RK (RC209748, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53RK.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53RK (RC209748, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53RK.(1:500)
WB Suggested Anti-TP53RK Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HT1080 cell lysate
Sample Type: Rat thyrocytes-FRTL-5 Primary Antibody Dilution: 1: 100 Secondary Antibody: Anti-rabbit-FITC Secondary Antibody Dilution: 1: 100 Color/Signal Descriptions: Green: TP53RK Blue: DAPI; Gene Name: TP53RK; Submitted by: Syed A Morshed, Mount Sina
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA808232 | TP53RK antibody |
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| TP53RK mouse monoclonal antibody, clone OTI5B2 (formerly 5B2) | |||||||
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Mouse | IgG2b | OTI5B2 | Purified | Hu | P, WB |
0.1 ml /
$360.00
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
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