TLX3 antibody
Principal name
TLX3 antibody
Alternative names for TLX3 antibody
TLX-3, T-cell leukemia homeobox protein 3, HOX11L2, Hox-11L2
Gene ID
30012 (TLX3)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P)
Background of TLX3 antibody
- Kit Component:
- - KN205739G1, TLX3 gRNA vector 1 in pCas-Guide vector
- KN205739G2, TLX3 gRNA vector 2 in pCas-Guide vector
- KN205739D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western blot analysis of TLX3 over-expressed 293 cell line, cotransfected with TLX3 Validated Chimera RNAi ( Cat # H00030012-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TLX3 monoclonal antibody (M01), clone 2A3 (Cat # H00030012-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of TLX3 expression in transfected 293T cell line by TLX3 monoclonal antibody (M01), clone 2A3.Lane 1: TLX3 transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.
TLX3 monoclonal antibody (M01), clone 2A3 Western Blot analysis of TLX3 expression in MCF-7 ( Cat # L046V1 ).
Detection limit for recombinant GST tagged TLX3 is approximately 0.03ng/ml as a capture antibody.
Detection limit for recombinant GST tagged TLX3 is approximately 0.03ng/ml as a capture antibody.
Detection limit for recombinant GST tagged TLX3 is approximately 0.03ng/ml as a capture antibody.
TLX3 MaxPab polyclonal antibody. Western Blot analysis of TLX3 expression in human spleen.
Western Blot analysis of TLX3 expression in transfected 293T cell line (H00030012-T01) by TLX3 MaxPab polyclonal antibody.Lane 1: TLX3 transfected lysate(32.01 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of TLX3 transfected lysate using anti-TLX3 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TLX3 MaxPab mouse polyclonal antibody (B01) (H00030012-B01).
Human ACHN; WB Suggested Anti-TLX3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: ACHN cell lysate; TLX3 antibody - N-terminal region (ARP31925_P050) in Human ACHN cells using Western Blot
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-TLX3 Rabbit Polyclonal antibody. (TA590472)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-TLX3 Rabbit Polyclonal antibody. (TA590472)
Immunohistochemistry: TLX3 Antibody - Normal and malignant tissues showed moderate to strong nuclear cytoplasmic staining. Gastro-intestinal glandular cells, urothelial cells, cells of seminiferus duct and tonsil exhibited strong nuclear and cytoplasmic staining. Placenta and exocrine pancreas displayed strong nuclear staining. Malignancies displayed in most cases similar staining as the corresponding normal tissue. Image and statement courtesy of the Human Protein Atlas (HPA).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TLX3 Rabbit Polyclonal antibody. (TA590472)
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-TLX3 Rabbit Polyclonal antibody. (TA590472)
Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-TLX3 Rabbit Polyclonal antibody. (TA590472)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TLX3 (RC205739, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TLX3.
ELISA: TLX3 Antibody
Western blot analysis of TLX3 in human spleen tissue lysate with TLX3 antibody at (A) 0.5 and (B) 1 ug/mL.
Immunohistochemistry of TLX3 in human spleen tissue with TLX3 antibody at 2.5 ug/mL.
Immunofluorescence of TLX3 in human spleen tissue with TLX3 antibody at 20 ug/mL.
WB Suggested Anti-TLX3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: ACHN cell lysate
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA590472 | TLX3 antibody |
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| Rabbit Polyclonal TLX3 Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, P, WB |
0.1 mg /
$409.00
|
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| OriGene Technologies, Inc. | |||||||
 +8 additional images |
Highly validated antibody! |
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| TA319908 | TLX3 antibody |
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| Rabbit Polyclonal TLX3 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 mg /
$415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305739 | TLX3 |
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| Recombinant protein of human T-cell leukemia homeobox 3 (TLX3) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY412148 | TLX3 overexpression lysate |
|
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0.1 mg /
$295.00
|
|
| OriGene Technologies, Inc. |