TIRAP antibody
Principal name
TIRAP antibody
Alternative names for TIRAP antibody
MAL, Toll/interleukin-1 receptor domain-containing adapter protein, TIR domain-containing adapter protein, MyD88 adapter-like protein, Adaptor protein Wyatt
Ncbi ID
NP_001034750.1, 9606, 10090, NP_683708, NP_001034750, NP_683708.1, AAL05627, NP_473437.2, NM_001039661, NM_148910
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Enzyme Immunoassay (E), Immunoprecipitation (IP)
Background of TIRAP antibody
- Kit Component:
- - KN317596G1, Tirap gRNA vector 1 in pCas-Guide vector
- KN317596G2, Tirap gRNA vector 2 in pCas-Guide vector
- KN317596D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Andreakos E, Sacre SM, Smith C, Lundberg A, Kiriakidis S, Stonehouse T, Monaco C, Feldmann M, Foxwell BM.
Distinct pathways of LPS-induced NF-kappa B activation and cytokine production in human myeloid and nonmyeloid cells defined by selective utilization of MyD88 and Mal/TIRAP.
Blood. 2004 Mar 15;103(6):2229-37.
PMID: 14630816
Immunohistochemical staining of human heart with Rabbit anti Mouse TIRAP antibody Cat.-No AP05881PU-N
Western blot analysis of human heart lysate probed with Rabbit anti Mouse TIRAP antibody Cat.-No AP05881PU-N at 0.5(A), 1(B) and 2(C) µg/ml
Western Blot analysis of TIRAP expression in transfected 293T cell line (H00114609-T01) by TIRAP MaxPab polyclonal antibody.Lane 1: TIRAP transfected lysate(28.27 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of TIRAP in human heart cell lysates with TIRAP antibody at (A) 0.5, (B) 1, and (C) 2 µg/ml.
Western blot analysis of TIRAP in MCF-7 cell lysate with TIRAP antibody (IN) at 4 µg/ml.
Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
Heart: Formalin-Fixed Paraffin-Embedded (FFPE)
Spleen: Formalin-Fixed Paraffin-Embedded (FFPE)
TIRAP polyclonal antibody ( Cat # PAB6059 ) staining ( 1 µg/ml ) of human liver lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
TIRAP polyclonal antibody ( Cat # PAB6892 ) ( 0.1 µg/ml ) staining of K-562 cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of TIRAP. Using TIRAP monoclonal antibody, clone 20D1960.2.1 ( Cat # MAB0073 ) at 1 : 1000 on 20 µgs of human spleen lysate.
Skeletal muscle: Formalin-Fixed Paraffin-Embedded (FFPE)
Western blot using Tirap polyclonal antibody ( Cat # PAB11331 ) shows detection of a predominant band at ~34 kDa corresponding to Tirap ( arrowhead ) in human heart tissue whole cell lysate.The predicted MW of Tirap is 26 kDa. Tirap was detected using 0.5 µg/ml ( lane A ) , 1.0 µg/ml ( lane B ) and 2.0 µg/ml ( lane C ) concentrations of primary antibody.
Immunohistochemical staining of human heart cellsusing Tirap polyclonal antibody ( Cat # PAB13113 ) at 2 µg/ml.
Western blot analysis of Tirap in human heart tissue lysates with Tirap polyclonal antibody ( Cat # PAB13113 ) at ( A ) 0.5, ( B ) 1, and ( C ) 2 µg/ml.
Western blot analysis of TIRAP expression in MCF -7 cell lysate with TIRAP polyclonal antibody ( Cat # PAB9723 ) at 4 µg/ml.
Western blot analysis of Tirap expression in human heart tissue with Tirap polyclonal antibody at 2 µg/ml.
Human Placenta; WB Suggested Anti-TIRAP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Placenta; TIRAP antibody - middle region (ARP52870_P050) in Human Placenta cells using Western Blot
TA302730 (1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA303067 (0.1µg/ml) staining of K562 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of TIRAP in MCF-7 cell lysate with TIRAP antibody at 4 ug/ml.
Immunohistochemistry of TIRAP in human heart tissue with TIRAP antibody at 20 ug/ml.
Immunofluorescence of TIRAP in Human Heart cells with TIRAP antibody at 20 ug/mL.
Western blot analysis of TIRAP in human heart cell lysates with TIRAP antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Immunohistochemical staining of human heart cells using TIRAP antibody at 2 ug/ml.
Immunofluorescence of TIRAP in Human Heart tissue with TIRAP antibody at 10 ug/mL.
Western Blot: TIRAP (TLR2 and TLR4) Antibody (20D1055.1) [TA336468] - analysis of TIRAP in human spleen lysate using this antibody.
WB Suggested Anti-TIRAP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Placenta
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (TIRAP Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (TIRAP Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307162 | TIRAP (transcript variant 3) |
||||
| Recombinant protein of human toll-interleukin 1 receptor (TIR) domain containing adaptor protein (TIRAP), transcript variant 3 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
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| OriGene Technologies, Inc. | |||||