TFE3 antibody
Principal name
TFE3 antibody
Alternative names for TFE3 antibody
Transcription factor E3
Ncbi ID
9606, NP_006512, NP_006512.2, NM_172472, NM_001105196, NM_006521
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of TFE3 antibody
- Kit Component:
- - KN206840G1, TFE3 gRNA vector 1 in pCas-Guide vector
- KN206840G2, TFE3 gRNA vector 2 in pCas-Guide vector
- KN206840D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Henthorn PS, Stewart CC, Kadesch T, Puck JM.
The gene encoding human TFE3, a transcription factor that binds the immunoglobulin heavy-chain enhancer, maps to Xp11.22. Genomics. 1991 Oct;11(2):374-8. PMID: 1685140
TFE3 MaxPab rabbit polyclonal antibody. Western Blot analysis of TFE3 expression in mouse kidney.
Western Blot analysis of TFE3 expression in transfected 293T cell line (H00007030-T02) by TFE3 MaxPab polyclonal antibody.Lane 1: TFE3 transfected lysate(61.50 KDa).Lane 2: Non-transfected lysate.
Human NCI-H226; WB Suggested Anti-TFE3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: NCI-H226 cell lysate. TFE3 is supported by BioGPS gene expression data to be expressed in NCIH226.; TFE3 antibody - middle region (ARP33440_P050) in Human NCI-H226 cells using Western Blot
Human MCF-7; WB Suggested Anti-TFE3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; TFE3 antibody - middle region (ARP33439_P050) in Human MCF-7 cells using Western Blot
Immunofluorescent analysis of TFE3 staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of TFE3 expression in HeLa (A); mouse lung (B); PC12 (C) whole cell lysates.
ELISA: Transcription factor E3 Antibody
Sample(30 ug whole cell lysate). A:Hep G2 . 7.5% SDS PAGE. TA308771 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using TFE3(TA308771) antibody at 1:200 dilution.
TFE3 antibody [N1], N-term detects TFE3 protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. TFE3 antibody [N1], N-term (TA308771) dilution: 1:500.
TFE3 antibody [N1], N-term detects TFE3 protein at nucleus on rat middle brain by immunohistochemical analysis. Sample: Paraffin-embedded rat middle brain. TFE3 antibody [N1], N-term (TA308771) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Transcription factor E3(TA308771) antibody at 1:200 dilution.
(1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of extracts from K562 cells and A549 cells, using TFE3 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using TFE3 antibody.The picture on the right is treated with the synthesized peptide.
Predicted band size: 62 kDa . Positive control: 293 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
TFE3 Antibody (N-term) (Cat. #TA324646) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TFE3 Antibody detected the TFE3 protein (arrow).
TFE3 Antibody (N-term) (Cat. #TA324646) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the TFE3 antibody detected the TFE3 protein (arrow).
IF image of Hela cell stained with TFE3 Antibody (N-term)(Cat#TA324646). Hela cells were incubated with TFE3 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . TFE3 immunoreactivity is localized to nucleus and Cytoplasm significantly.
Immunohistochemistry staining of Paraffin Melanoma, ASPS tissue by TFE3 antibody (dilution: 1:50 - 1:200; visualization of staining: Nuclear)
WB Suggested Anti-TFE3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: MCF7 cell lysate
WB Suggested Anti-TFE3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: NCI-H226 cell lysateTFE3 is supported by BioGPS gene expression data to be expressed in NCIH226
Western blot analysis on K562 cell lysate using TFE3 Antibody
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP710034 | TFE3 (N-term polyhistidine tag) |
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| Recombinant protein of human transcription factor binding to IGHM enhancer 3 (TFE3),with N-terminal polyhistidine tag, expressed in sf9 cells. | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
SF9 cells |
20 µg /
$411.00
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| OriGene Technologies, Inc. | |||||