TBX5 antibody
Principal name
TBX5 antibody
Alternative names for TBX5 antibody
T-box protein 5, T-box transcription factor TBX5
SwissProt ID
P70326 (Mouse), Q5I2P1 (Rat), Q99593 (Human), Q9IAK8 (Danre), Q9PWE8 (Chick), Q9W7C2 (Xenla)
Ncbi ID
NP_000183, 9606, NP_542449, NP_000183.2, NM_000192, NM_181486, NM_011537, NM_001009964
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection))
Background of TBX5 antibody
- Kit Component:
- - KN206804G1, TBX5 gRNA vector 1 in pCas-Guide vector
- KN206804G2, TBX5 gRNA vector 2 in pCas-Guide vector
- KN206804D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Riazi,A.M., et al., (2005) J. Biol. Chem. 280 (11), 10716-10720
Human Brain, cortex; Immunohistochemistry with Human Brain, cortex tissue at an antibody concentration of 5.0ug/ml using anti-TBX5 antibody (ARP33403_P050); TBX5 antibody - N-terminal region (ARP33403_P050) in Human Brain, cortex cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-TBX5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; TBX5 antibody - N-terminal region (ARP33403_P050) in Human HepG2 cells using Western Blot
Western blot analysis of TBX5 over-expressed 293 cell line, cotransfected with TBX5 Validated Chimera RNAi ( Cat # H00006910-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TBX5 monoclonal antibody (M01), clone 1G10 (Cat # H00006910-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of TBX5 expression in transfected 293T cell line by TBX5 monoclonal antibody (M01), clone 1G10.Lane 1: TBX5 transfected lysate(57.7 KDa).Lane 2: Non-transfected lysate.
Human muscle; Rabbit Anti-TBX5 Antibody. Catalog Number: ARP33402. Paraffin Embedded Tissue: Human Muscle. Cellular Data: Skeletal muscle cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; TBX5 antibody - middle region (ARP33402_P050) in Human muscle cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-TBX5 Antibody Titration: 0.5ug/ml. Positive Control: Jurkat cell lysate; TBX5 antibody - middle region (ARP33402_P050) in Human Jurkat cells using Western Blot
Human HepG2; WB Suggested Anti-TBX5 Antibody. . Titration: 5 ug/ml. . Positive Control: HepG2 Whole Cell; TBX5 antibody - N-terminal region (ARP33403_T100) in Human HepG2 cells using Western Blot
TBX5 MaxPab rabbit polyclonal antibody. Western Blot analysis of TBX5 expression in mouse brain.
TBX5 MaxPab rabbit polyclonal antibody. Western Blot analysis of TBX5 expression in human kidney.
TBX5 Antibody (N-term) (Cat. #AP54186PU-N) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the TBX5 antibody detected the TBX5 protein (arrow).
Western blot analysis using TBX5 antibody Cat.-No against HepG2 cell lysate (1).
WB Suggested Anti-TBX5 Antibody Titration: 0.5 ug/ml; Positive Control: Jurkat cell lysate
Rabbit Anti-TBX5 Antibody; Paraffin Embedded Tissue: Human Muscle; Cellular Data: Skeletal muscle cells; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
WB Suggested Anti-TBX5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate
Immunohistochemistry with Human Brain, cortex tissue at an antibody concentration of 5.0 ug/ml using anti-TBX5 antibody
WB Suggested Anti-TBX5 Antibody; Titration: 5 ug/ml; Positive Control: HepG2 Whole Cell
Host: Rabbit; Target Name: Tbx5; Sample Tissue: Mouse Pancreas lysates; Antibody Dilution: 1.0 ug/ml
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM06346SU-N | TBX5 antibody |
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Mouse | IgG1 | 7B11 | Ascites | Hu | WB |
0.1 ml /
$380.00
|
| OriGene Technologies GmbH | |||||||
| AP54186PU-N | TBX5 (N-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
$415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP316520 | TBX5 (transcript variant 1) |
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| Recombinant protein of human T-box 5 (TBX5), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY400071 | TBX5 overexpression lysate |
|
 |
0.1 mg /
$295.00
|
|
| OriGene Technologies, Inc. | ||
| LY403620 | TBX5 overexpression lysate |
|
|
0.1 mg /
$495.00
|
|
| OriGene Technologies, Inc. | ||
| LY424987 | TBX5 overexpression lysate |
|
|
0.1 mg /
$295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR205420L2V | Lenti ORF particles, Tbx5 (GFP-tagged ORF) - Rat T-box 5 (Tbx5), 200 uL, >10^7 TU/mL |
|
|
200 µl /
$1,210.00
|
|
| OriGene Technologies, Inc. | ||
| RR205420L1V | Lenti ORF particles, Tbx5 (Myc-DDK-tagged ORF) - Rat T-box 5 (Tbx5), 200 uL, >10^7 TU/mL |
|
|
200 µl /
$1,080.00
|
|
| OriGene Technologies, Inc. | ||