SPDEF antibody
Principal name
SPDEF antibody
Alternative names for SPDEF antibody
PDEF, PSE, SAM pointed domain-containing Ets transcription factor, Prostate-derived Ets factor, Prostate epithelium-specific Ets transcription factor
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of SPDEF antibody
- Kit Component:
- - KN205688G1, SPDEF gRNA vector 1 in pCas-Guide vector
- KN205688G2, SPDEF gRNA vector 2 in pCas-Guide vector
- KN205688D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Fowler,M., (2006) J. Cell. Biochem. 97 (1), 1-17
Western Blot analysis of SPDEF expression in transfected 293T cell line by SPDEF monoclonal antibody (M01), clone 4A5.Lane 1: SPDEF transfected lysate(37.518 KDa).Lane 2: Non-transfected lysate.
Human MCF7; WB Suggested Anti-SPDEF Antibody Titration: 2.5ug/ml. ELISA Titer: 1:12500. Positive Control: MCF7 cell lysate. SPDEF is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells.; SPDEF antibody - N-terminal region (ARP31841_T100) in Human MCF7 cells using Western Blot
Human Placenta; WB Suggested Anti-SPDEF Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Placenta; SPDEF antibody - middle region (ARP32533_P050) in Human Placenta cells using Western Blot
Human HeLa; WB Suggested Anti-SPDEF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate; SPDEF antibody - N-terminal region (ARP32534_P050) in Human HeLa cells using Western Blot
Human Fetal Muscle; WB Suggested Anti-SPDEF Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Muscle; SPDEF antibody - C-terminal region (ARP38992_P050) in Human Fetal Muscle cells using Western Blot
Human MCF7; WB Suggested Anti-SPDEF Antibody Titration: 2.5ug/ml. ELISA Titer: 1:12500. Positive Control: MCF7 cell lysate. SPDEF is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells.; SPDEF antibody - N-terminal region (AP42057PU-N) in Human MCF7 cells using Western Blot
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human tonsil and colon cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human tonsil tissue using SPDEF antibody at dilution 1/60, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human tonsil and colon cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using SPDEF antibody at dilution 1/60, on the right is treated with the fusion protein. (Original magnification:x200)
WB Suggested Anti-SPDEF Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Placenta
WB Suggested Anti-SPDEF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Hela cell lysate
WB Suggested Anti-SPDEF Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Muscle
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA324209 | SPDEF antibody |
||||||
| Anti-SPDEF Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
$335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305688 | SPDEF |
||||
| Recombinant protein of human SAM pointed domain containing ets transcription factor (SPDEF) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY415796 | SPDEF overexpression lysate |
|
 |
0.1 mg /
$295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR204725L2V | Lenti ORF particles, Spdef (GFP-tagged) - Mouse SAM pointed domain containing ets transcription factor (Spdef), 200 uL, >10^7 TU/mL |
|
|
200 µl /
$930.00
|
|
| OriGene Technologies, Inc. | ||
| MR204725L1V | Lenti ORF particles, Spdef (Myc-DDK-tagged) - Mouse SAM pointed domain containing ets transcription factor (Spdef), 200 uL, >10^7 TU/mL |
|
|
200 µl /
$830.00
|
|
| OriGene Technologies, Inc. | ||