SOX10 antibody
Principal name
SOX10 antibody
Alternative names for SOX10 antibody
Transcription factor SOX-10, SRY-box 10, DOM
Ncbi ID
9606, NP_008872, NP_008872.1, XM_128139, NP_035567.1, BC018551, BC023356, NM_011437, NM_019193, NM_006941
Available reactivities
Available hosts
Available applications
Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of SOX10 antibody
- Kit Component:
- - KN316487G1, Sox10 gRNA vector 1 in pCas-Guide vector
- KN316487G2, Sox10 gRNA vector 2 in pCas-Guide vector
- KN316487D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunoperoxidase of monoclonal antibody to SOX10 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml]
Human Skin; Immunohistochemistry with Skin tissue at an antibody concentration of 4-8ug/ml using anti-SOX10 antibody (ARP33326_P050); SOX10 antibody - middle region (ARP33326_P050) in Human Skin cells using Immunohistochemistry
human optic nerves and spinal cord; A/ [IHC-PZ] (optimal processing)human optic nerve (short post mortem interval) fixed by immersion in zinc-based fixative (BD Pharmingen 552658), processed to minimize antigen loss (shortened protocol, reduced exposure to high temperature); embedded in paraffin wax; sectioned at 3 micronsA2/ [IHC-PZ + FF]As above for initial fixation then post-fixed in 10% buffered formalin for 5 daysB/ [IHC-P]human optic nerve and spinal cord fixed in 10% buffered formalin (relatively short post mortem interval and fixation duration); standard processing; embedded in paraffin wax; sectioned at 3 micronsControlsNegative: omission of primary.Positive: Olig2 reactivity was in comparison with antibody from Prof Charles Stiles/ Dr John Alberta (DF308; N terminal Olig2) used at 1:10000 (IHC-PZ); 1:4000 (IHC-P); SOX10 antibody - middle region (ARP33326_P050) in Human human optic nerves and spinal cordcells using Immunohistochemistry
Human Kidney; Immunohistochemistry with Kidney lysate tissue at an antibody concentration of 4-8ug/ml using anti-SOX10 antibody (ARP33326_P050); SOX10 antibody - middle region (ARP33326_P050) in Human Kidney cells using Immunohistochemistry
Human Jurkat; Host: Rabbit. Target Name: SOX10. Sample Tissue: Jurkat cell lysates. Lane A: Primary Antibody . Lane B: Primary Antibody + Blocking Peptide . Primary Antibody Concentration:2.5ug/ml. Peptide Concentration: 4.0ug/ml. Lysate Quantity: 25ug/lane. Gel Concentration: 12%.; SOX10 antibody - middle region (ARP33326_P050) in Human Jurkat cells using Western Blot
Human HepG2; WB Suggested Anti-SOX10. Antibody Titration: .25ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; SOX10 antibody - middle region (ARP33326_P050) in Human HepG2 cells using Western Blot
Human HEK293; SOX10 antibody - middle region (ARP33326_P050) validated by WB using Hek 293 Whole Cell Lysate at 1:4,000.; SOX10 antibody - middle region (ARP33326_P050) in Human HEK293 cells using Western Blot
Mouse SP2/0; WB Suggested Anti-Sox10 Antibody Titration: 0.2-1 ug/ml. Positive Control: SP2/0 cell lysate; Sox10 antibody - N-terminal region (ARP37633_P050) in Mouse SP2/0 cells using Western Blot
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP32967PU-N (0.1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
WB Suggested Anti-SOX10Antibody Titration: 0.25 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate
SOX10 antibody - middle region validated by WB using Hek 293 Whole Cell Lysate at 1:4,000.
Host: Rabbit; Target Name: SOX10; Sample Tissue: Jurkat cell lysates; Lane A: Primary Antibody; Lane B: Primary Antibody + Blocking Peptide ; Primary Antibody Concentration:2.5 ug/ml; Peptide Concentration: 4.0ug/ml; Lysate Quantity: 25ug/lane; Gel Concen
Immunohistochemistry with Skin tissue at an antibody concentration of 4-8 ug/ml using anti-SOX10 antibody
Immunohistochemistry with Kidney lysate tissue at an antibody concentration of 4-8 ug/ml using anti-SOX10 antibody
A/ [IHC-PZ] (optimal processing) human optic nerve (short post mortem interval) fixed by immersion in zinc-based fixative (BD Pharmingen 552658), processed to minimize antigen loss (shortened protocol, reduced exposure to high temperature); embedded in pa
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP32967PU-N | SOX10 antibody |
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Goat | Aff - Purified | Bov, Can, Hu, Ms, Por, Rt | WB |
0.1 mg /
$380.00
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
OriGene recommends this product for Western blotting. |
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