Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Target » SOCS1 antibody

SOCS1 antibody

(reset)

Principal name

SOCS1 antibody

Alternative names for SOCS1 antibody

Suppressor of cytokine signaling 1, SOCS-1, SSI1, SSI-1, TIP3, TIP-3, JAB, JAK-binding protein, STAT-induced STAT inhibitor 1, Tec-interacting protein 3

SwissProt ID

O15524 (Human), O35716 (Mouse), Q9QX78 (Rat)

Gene ID

8651 (SOCS1), 252971 (Socs1), 12703

Ncbi ID

9606, NP_003736, NP_003736.1, 10116, NM_003745, AAI53089.1, CAB92528, NM_009896, NM_145879

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Chk (Chicken), Rt (Rat), Rb (Rabbit), Bov (Bovine), Por (Porcine)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SOCS1 antibody

Suppressor of cytokine signaling 1 (SOCS1) is a member of the SOCS family of proteins, consisting of eight members (SOCS1 to SOCS7 and CIS) all sharing a central SH2 domain and a c-terminal SOCS box (1). This family is involved in inducible feedback inhibition of janus kinases (JAKs) and signal transducers and activators of transcription (STATs) signaling pathway, which plays multiple roles in cell growth, differentiation, and apoptosis (2). SOCS1 is located on chromosome 16 (3). It plays a key role in the negative regulation of IFN-gamma signaling and in T-cell differentiation. SOCS1 inhibits macrophage differentiation of M1 cells in response to IL-6, protects protein tyrosine phosphatases by thioredoxin upregulation and attenuates JAKs to suppress ROS-mediated apoptosis (2, 4). It translocates into the nucleus due to the presence of a functional nuclear localization signal (NLS). It also regulates IFN-gamma mediated sensory neuronal survival.
SOCS1 is implicated in various cancers and diseases. Mutations of SOCS1 are found in classic Hodgkin lymphoma and primary mediastinal B-cell lymphoma (5). It is induced in response to TNF-alpha or H2O2 and by a subset of cytokines including those belonging to interferon, interleukin and colony stimulating factor colonies. It attenuates their signaling by its ability to bind and inhibit all four of the JAKs. It is found to colocalize and biochemically copurify with the microtubule organizing complex (MTOC) and its associated 20S proteasome (6). It is expressed in all tissues with high expression in spleen, small intestine and peripheral blood lymphocytes. It is approximately a 25kDa protein (211 amino acids).

General readings

1. Larsen L, Ropke C. Suppressors of Cytokine Signaling: SOCS. APMIS. December 2002; 110(12):833-844.
2. Oh J, Hur MW, Lee CE. SOCS1 protects protein tyrosine phosphatases by thioredoxin upregulation and attenuates JAK to suppress ROS mediated apoptosis. Oncogene. 3 September 2009; 28(35):3145-3156.
3. Yandava CN, Pillari A, Drazen JM. Radiation hybrid and cytogenetic mapping of SOCS1 and SOCS2 to chromosomes 16p13 and 12q, respectively. Genomics. 1 October 1999; 61(1):108-111.
4. Starr R, Wilson TA, Viney EM, Murray LJ, Rayner JR, Jenkins BJ, Gonda TJ, Alexander WS, Metcalf D, Nicola NA, Hilton DJ. A family of cytokine-inducible inhibitors of signaling. Nature. 26 June 1997; 6636:917-921.
5. Weniger MA, Melzner I, Menz CK, Wegener S, Bucur AJ, Dorsch K, Mattfeldt T, Barth TF, Muller P. Mutations of the tumor suppressor gene SOCS-1 in classical Hodgkin lymphoma are frequent and associated with delayed degradation of JAK2 protein. Oncogene. 2006; 25(18):2679-2684.
6. Vuong BQ, Arenzana TL, Showalter BM, Losman J, Chen XP, Mostecki J, Banks AS, Limnander A, Fernandez N, Rothman PB. SOCS-1 localizes to the microtubule organizing complexassociated
20S proteasome. Mol Cell Biol. 2004; 24(20):9092-9101.

PAB0214 - SOCS1
Western blot analysis of SOCS1. Using SOCS1 polyclonal antibody ( Cat # PAB0214 ) at 1 : 1000 dilution against 15 ug/lane of Ramos ( A ) and Raw ( B ) cell lysate.
PAB6012 - SOCS1
SOCS1 polyclonal antibody ( Cat # PAB6012 ) staining ( 2 µg/ml ) of mouse spleen lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
ARP42147_T100 - SOCS1
Human Kidney; Rabbit Anti-SOCS1 Antibody. Catalog Number: ARP42147. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; SOCS1 antibody - N-terminal region (ARP42147_T100) in Human Kidney cells using Immunohistochemistry
ARP42147_T100 - SOCS1
Mouse, Rat; . Lanes: 1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug). Primary Antibody Dilution: 2ug/ml. Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience. Secondary Antibody Dilution: 1: 20,000. Gene Name: SOCS1. Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science.; SOCS1 antibody - N-terminal region (ARP42147_T100) in Mouse, Rat cells using Western Blot
ARP42147_T100 - SOCS1
Huh7 transfected with microRNA; SOCS1 antibody - N-terminal region (ARP42147_T100) validated by WB using Huh7 Cells transfected with microRNA at 1:1000.; SOCS1 antibody - N-terminal region (ARP42147_T100) in Huh7 transfected with microRNA cells using Western Blot
ARP42147_T100 - SOCS1
Human Jurkat; SOCS1 antibody - N-terminal region (ARP42147_T100) validated by WB using Jurkat cell lysate at 0.2-1 ug/ml.; SOCS1 antibody - N-terminal region (ARP42147_T100) in Human Jurkat cells using Western Blot
ARP42147_T100 - SOCS1
Human Jurkat; WB Suggested Anti-SOCS1 Antibody Titration: 1.25ug/ml. . Sample Type: Jurkat Lysate (20ug) Sample Type: Jurkat cell lysate; SOCS1 antibody - N-terminal region (ARP42147_T100) in Human Jurkat cells using Western Blot
PAB12778 - SOCS1
The tissue/cell lysate derived from mouse liver ( Lane 1 ), mouse muscle ( Lane 2 ) and MCF-7 ( Lane 3 ) were immunoprobed by SOCS1 polyclonal antibody ( Cat # PAB12778 ) at 1 : 500. An immunoreactive band is observed around ~24kDa.
PAB13191 - SOCS1
Western blot analysis of SOCS1 in human spleen tissue lysate with SOCS1 polyclonal antibody ( Cat # PAB13191 ) at ( A ) 1, ( B ) 2 and ( C ) 4 µg/ml.
PAB13192 - SOCS1
Western blot analysis of SOCS1 in human spleen tissue lysate with SOCS1 polyclonal antibody ( Cat # PAB13192 ) at ( A ) 1, ( B ) 2 and ( C ) 4 µg/ml.
PAB8011 - SOCS1
Immunohistochemical analysis of paraffin-embedded rat lung sections, stain SOCS1 in cytoplasm DAB chromogenic reaction.
PAB9029 - SOCS1
Western blot analysis of Socs1 expression in Jurkat cell lysate ( Lane 1, 2 ) .
AP08926PU-N - SOCS1
Brain, cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP08926PU-N - SOCS1
Western blot analysis of SOCS1 in Human spleen cell lysate with SOCS1 antibody at (A) 1, (B) 2 and (C) 4 µg/ml.
AP08927PU-N - SOCS1
Brain, cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP08927PU-N - SOCS1
Western blot analysis of SOCS1 in Human spleen cell lysate with SOCS1 antibody at (A) 1, (B) 2 and (C) 4 µg/ml.
AP18204PU-N - SOCS1
Western blot analysis of SOCS1 Antibody (N-term) (AP18204PU-N) in mouse kidney tissue lysates (35ug/lane). SOCS1 (arrow) was detected using the purified Pab.
AP20630PU-N - SOCS1
Western blot analysis of SOCS-1 in extracts from Jurkat cells using SOCS1 / JAB antibody (Cat.-No. AP20630PU-N).
AP23370PU-N - SOCS1
Immunohistochemical analysis of paraffin-embedded rat tissue sections (lung), staining SOCS1 in nucleus, DAB chromogenic reaction
ARP42148_P050 - SOCS1
Human ACHN; WB Suggested Anti-SOCS1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: ACHN cell lysate; SOCS1 antibody - middle region (ARP42148_P050) in Human ACHN cells using Western Blot
AM26561AF-N - SOCS1

Western blot analysis of GST-SOCS-1 (2-78 a.a.) using AM26561AF-N.


AM26561AF-N - SOCS1

Immunoprecipitation of SOCS-1 from mouse splenocytes (1) and mouse thymocytes (3) with AM26561AF-N (1,3) or mouse IgG1 (2,4). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with biotinylated anti-SOCS-1 monoclonal antibody.


AM26561BT-N - SOCS1
Immunoprecipitation of SOCS-1 from mouse splenocytes with mouse IgG1 (1), AM26561AF-N (2), and from mouse hepatocytes with mouse IgG1 (3), AM26561AF-N (4). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM26561BT-N.
CPA3359-100ul - SOCS1
Immunofluorescent analysis of SOCS1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CPA3359-100ul - SOCS1
Western blot analysis of SOCS1 expression in MCF7 (A); mouse kidney (B) whole cell lysates.
TP320847 - SOCS1
TA302691 - SOCS1
TA302691 staining (2µg/ml) of mouse spleen lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA306303 - SOCS1
Western blot analysis of SOCS1 in Human spleen cell lysate with SOCS1 antibody at (A) 1, (B) 2 and (C) 4 µg/ml.
TA306304 - SOCS1
Western blot analysis of SOCS1 in Human spleen cell lysate with SOCS1 antibody at (A) 1, (B) 2 and (C) 4 µg/ml.
TA321592 - SOCS1
Predicted band size: 24 kDa . Positive control: 231 cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/1300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
TA321592 - SOCS1
Predicted cell location: Cytoplasm. Positive control: Human gastric cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using SOCS1 antibody at dilution 1/70, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA321593 - SOCS1
Predicted band size: 24kDa. Positive control: HT-29 and 231 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: HT-29 cell lysate Lane2: 231 cell lysate Lysates: 40 ug per lane Primary antibody: 1/1700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 1 minute)
TA321593 - SOCS1
Predicted cell location: Cytoplasm. Positive control: Human ovarian cancer tissue. Recommended dilution: 1/100-300 The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using SOCS1 antibody at dilution 1/90, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA336433 - SOCS1
Western Blot: SOCS1 Antibody [TA336433] - Western blot analysis of SOCS1 using TA336433 at 1:1000 dilution against 15 ug/lane of Ramos (A) and Raw (B) cell lysate.

14 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SOCS1 (C-term) antibody
  Goat Polyclonal Antibody against SOCS1  
TA302691 staining (2µg/ml) of mouse spleen lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, P, WB
0.1 mg / $360.00
  OriGene Technologies, Inc.

SOCS1 (C-term) antibody
  Rabbit Polyclonal SOCS1 Antibody  
Western blot analysis of SOCS1 in Human spleen cell lysate with SOCS1 antibody at (A) 1, (B) 2 and (C) 4 µg/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / $415.00
  OriGene Technologies, Inc.

SOCS1 (N-term) antibody
  Rabbit Polyclonal SOCS1 Antibody  
Western blot analysis of SOCS1 in Human spleen cell lysate with SOCS1 antibody at (A) 1, (B) 2 and (C) 4 µg/ml. Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / $415.00
  OriGene Technologies, Inc.

SOCS1 antibody
  Anti-SOCS1 rabbit polyclonal antibody  
Predicted band size: 24 kDa . Positive control: 231 cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/1300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / $335.00
  OriGene Technologies, Inc.
+1 additional image

SOCS1 antibody
  Anti-SOCS1 rabbit polyclonal antibody  
Predicted band size: 24kDa. Positive control: HT-29 and 231 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: HT-29 cell lysate Lane2: 231 cell lysate Lysates: 40 ug per lane Primary antibody: 1/1700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / $335.00
  OriGene Technologies, Inc.
+1 additional image

SOCS1 antibody
  Rabbit Polyclonal SOCS-1 Antibody  
Western Blot: SOCS1 Antibody [TA336433] - Western blot analysis of SOCS1 using TA336433 at 1:1000 dilution against 15 ug/lane of Ramos (A) and Raw (B) cell lysate. Rabbit Purified Hu, Ms WB
0.2 ml / $395.00
  OriGene Technologies, Inc.

SOCS1 antibody
Brain, cortex: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / $425.00
  OriGene Technologies GmbH
+1 additional image

SOCS1 antibody
Brain, cortex: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu E, P, WB
50 µg / $425.00
  OriGene Technologies GmbH
+1 additional image

SOCS1 (N-term) antibody
Western blot analysis of SOCS1 Antibody (N-term) (AP18204PU-N) in mouse kidney tissue lysates (35ug/lane). SOCS1 (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / $415.00
  OriGene Technologies GmbH

SOCS1 antibody
Western blot analysis of SOCS-1 in extracts from Jurkat cells using SOCS1 / JAB antibody (Cat.-No. AP20630PU-N). Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / $370.00
  OriGene Technologies GmbH

SOCS1 (C-term) antibody
Immunohistochemical analysis of paraffin-embedded rat tissue sections (lung), staining SOCS1 in nucleus, DAB chromogenic reaction Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / $390.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SOCS1
  Recombinant protein of human suppressor of cytokine signaling 1 (SOCS1)  
SOCS1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / $748.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Socs1 (GFP-tagged ORF) - Rat suppressor of cytokine signaling 1 (Socs1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Socs1 (GFP-tagged ORF) - Rat suppressor of cytokine signaling 1 (Socs1), 200 uL, >10^7 TU/mL  
200 µl / $930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Socs1 (Myc-DDK-tagged ORF) - Rat suppressor of cytokine signaling 1 (Socs1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Socs1 (Myc-DDK-tagged ORF) - Rat suppressor of cytokine signaling 1 (Socs1), 200 uL, >10^7 TU/mL  
200 µl / $830.00
  OriGene Technologies, Inc.
 

14 Item(s)

per page
14 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Primary Antibody (11)
  2. Protein | Growth Factor (1)
  3. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (11)
Target
  1. SOCS1 (12)
Property
  1. C-term (3)
  2. N-term (2)
Reactivity
  1. Human (Hu) (11)
  2. Mouse (Ms) (9)
  3. Rat (Rt) (7)
  4. Bovine (Bov) (1)
  5. Canine (Can) (1)
Applications
  1. Western blot / Immunoblot (WB) (11)
  2. Enzyme Immunoassay (E) (7)
  3. Paraffin Sections (P) (6)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Aff - Purified (7)
  2. Purified (3)
Host
  1. Rabbit (10)
  2. Goat (1)
Images
  1. 1 image (12)
Images: Applications
  1. Western blot / Immunoblot (WB) (10)
  2. Paraffin Sections (P) (3)
  3. IHC (2)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (14)
Service
 
© Copyright 1998 - 2018 OriGene Technologies, Inc.
  • LinkedIn