SMYD2 antibody
Principal name
SMYD2 antibody
Alternative names for SMYD2 antibody
HSKM-B, KMT3C, SET and MYND domain-containing protein 2, Lysine N-methyltransferase 3C
Ncbi ID
9606, NP_064582, NP_064582.2, NM_020197, NP_081072.1, NM_026796
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), ChIP assay (ChIP), Paraffin Sections (P)
Background of SMYD2 antibody
- Kit Component:
- - KN316363G1, Smyd2 gRNA vector 1 in pCas-Guide vector
- KN316363G2, Smyd2 gRNA vector 2 in pCas-Guide vector
- KN316363D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western blot analysis of SYMD2 ( arrow ) using rabbit SMYD2 polyclonal antibody ( Cat # PAB2223 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the SYMD2 gene ( Lane 2 ) ( Origene Technologies ) .
Western Blot analysis of SMYD2 expression in transfected 293T cell line (H00056950-T02) by SMYD2 MaxPab polyclonal antibody.Lane 1: SMYD2 transfected lysate(47.63 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of SMYD2 expression in transfected 293T cell line (H00056950-T02) by SMYD2 MaxPab polyclonal antibody.Lane 1: SMYD2 transfected lysate(49.70 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of SMYD2 expression in transfected 293T cell line (H00056950-T02) by SMYD2 MaxPab polyclonal antibody.Lane 1: SMYD2 transfected lysate(49.70 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of SMYD2 transfected lysate using anti-SMYD2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SMYD2 MaxPab mouse polyclonal antibody (B01) (H00056950-B01).
Human ACHN; WB Suggested Anti-SMYD2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: ACHN cell lysate. SMYD2 is supported by BioGPS gene expression data to be expressed in ACHN.; SMYD2 antibody - N-terminal region (ARP48568_P050) in Human ACHN cells using Western Blot
Human SH-SYSY; WB Suggested Anti-SMYD2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: SH-SYSY cell lysate. SMYD2 is supported by BioGPS gene expression data to be expressed in SHSY5Y.; SMYD2 antibody - middle region (ARP50639_P050) in Human SH-SYSY cells using Western Blot
HCT116; Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.; SMYD2 antibody - middle region (ARP50639_P050) in HCT116 using CHIP
Western blot analysis of SMYD2 expression in HEK293T (A); HeLa (B) whole cell lysates.
Western blot analysis of SYMD2 (arrow) using rabbit polyclonal SYMD2 Antibody (N-term) (Cat.#TA302185).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SYMD2 gene (Lane 2) (Origene Technologies).
SMYD2 Antibody (N-term) (Cat.#TA302185) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the SMYD2 antibody detected the SMYD2 protein (arrow).
SMYD2 Antibody (N-term) (Cat.#TA302185) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the SMYD2 antibody detected the SMYD2 protein (arrow).
WB Suggested Anti-SMYD2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: SH-SYSY cell lysate SMYD2 is supported by BioGPS gene expression data to be expressed in SHSY5Y
Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
Western blot detection of KMT3C / SMYD2 in 3T3,HT1080,A431 and Jurkat cell lysates and using KMT3C / SMYD2 mouse mAb (1:1000 diluted). Predicted band size: 50KDa.Observed band size: 50KDa.
WB Suggested Anti-SMYD2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: ACHN cell lysate.SMYD2 is supported by BioGPS gene expression data to be expressed in ACHN
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302185 | SMYD2 (N-term) antibody |
||||||
| Rabbit Polyclonal Antibody against SMYD2 (N-term) | |||||||
 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
$415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|||||||
| TA347054 | SMYD2 antibody |
||||||
| Mouse Monoclonal KMT3C / SMYD2 Antibody | |||||||
 |
Mouse | IgG2b | 1G10-E11-H9 | Purified | WB |
0.1 ml /
$335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA351942 | SMYD2 antibody |
||||||
| Rabbit Polyclonal Anti-SMYD2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
$335.00
|
|
| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP314307 | SMYD2 |
||||
| Recombinant protein of human SET and MYND domain containing 2 (SMYD2) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY412619 | SMYD2 overexpression lysate |
|
|
0.1 mg /
$495.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC214307L1V | Lenti ORF particles, SMYD2 (Myc-DDK tagged) - Human SET and MYND domain containing 2 (SMYD2) , 200 uL, >10^7 TU/mL |
|
|
200 µl /
$1,000.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206911L2V | Lenti ORF particles, Smyd2 (GFP-tagged) - Mouse SET and MYND domain containing 2 (Smyd2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
$1,090.00
|
|
| OriGene Technologies, Inc. | ||
| MR206911L1V | Lenti ORF particles, Smyd2 (Myc-DDK-tagged) - Mouse SET and MYND domain containing 2 (Smyd2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
$980.00
|
|
| OriGene Technologies, Inc. | ||