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SMAD5 antibody

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Principal name

SMAD5 antibody

Alternative names for SMAD5 antibody

SMAD family member 5, SMAD 5, SMAD-5, MADH5, MAD homolog 5, JV5-1, Mothers against decapentaplegic homolog 5, Mothers against DPP homolog 5

SwissProt ID

P97454 (Mouse), Q56I99 (Chick), Q99717 (Human), Q9R1V3 (Rat), Q9W7E7 (Danre)

Gene ID

4090 (SMAD5), 17129 (Smad5), 59328

Ncbi ID

NP_001001419.1, 9606, NP_001001420, NP_005894, NP_001157513.1, NM_001164042, NM_008541, NM_021692

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Sh (Sheep)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), ELISA (detection) (E(detection)), Flow Cytometry (F), Paraffin Sections (P)

Background of SMAD5 antibody

Bone morphogenetic proteins (BMPs) constitute a large family of sigling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determition, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-β family of Ser/Thr kise receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-termil motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5).MAP kises and CDKs 8 and 9 phosphorylate residues in the linker region of Smad1, including Ser206. The phosphorylation of Ser206 recruits Smurf1 to the linker region and leads to the degradation of Smad1 (6). Phosphorylation of this site also promotes Smad1 transcriptiol action by recruiting YAP to the linker region (7).

General readings

Langenfeld,E.M., et al., (2006) Oncogene 25 (5), 685-692

H00004090-M01 - SMAD5
Immunofluorescence of monoclonal antibody to SMAD5 on HeLa cell. [antibody concentration 10 ug/ml]
H00004090-M01 - SMAD5
SMAD5 monoclonal antibody (M01), clone 2D7 Western Blot analysis of SMAD5 expression in Hela NE ( Cat # L013V3 ).
H00004090-M01 - SMAD5
SMAD5 monoclonal antibody (M01), clone 2D7. Western Blot analysis of SMAD5 expression in K-562 ( Cat # L009V1 ).
H00004090-M01 - SMAD5
SMAD5 monoclonal antibody (M01), clone 2D7. Western Blot analysis of SMAD5 expression in Jurkat ( Cat # L017V1 ).
H00004090-M01 - SMAD5
Detection limit for recombinant GST tagged SMAD5 is approximately 0.1ng/ml as a capture antibody.
H00004090-M03 - SMAD5
Immunofluorescence of monoclonal antibody to SMAD5 on HeLa cell. [antibody concentration 10 ug/ml]
H00004090-M03 - SMAD5
SMAD5 monoclonal antibody (M03), clone 3A10 Western Blot analysis of SMAD5 expression in HeLa ( Cat # L013V1 ).
H00004090-M03 - SMAD5
Detection limit for recombinant GST tagged SMAD5 is approximately 0.3ng/ml as a capture antibody.
ARP32006_T100 - SMAD5
Human HepG2; WB Suggested Anti-SMAD5 Antibody. . Titration: 2.5 ug/ml. . Positive Control: HepG2 Whole Cell; SMAD5 antibody - middle region (ARP32006_T100) in Human HepG2 cells using Western Blot
ARP32006_P050 - SMAD5
r-SMAD5; . Lanes: Lane 1: 5ug of transfected 293T lysate (SMAD1) . Lane 1: 5ug of transfected 293T lysate (SMAD2) . Lane 1: 5ug of transfected 293T lysate (SMAD3) . Lane 1: 5ug of transfected 293T lysate (SMAD4) . Lane 1: 5ug of transfected 293T lysate (SMAD5) . Lane 1: 5ug of transfected 293T lysate (SMAD6) . Lane 1: 5ug of transfected 293T lysate (SMAD7) . Lane 1: 5ug of transfected 293T lysate (SMAD8) . Lane 1: 5ug of transfected 293T lysate (GFP). Primary Antibody Dilution: 1:1000. Secondary Antibody: Goat anti-Rabbit IgG HRP Conjugated. Secondary Antibody Dilution: 1:10,000. Gene Name: SMAD5. Submitted by: Amanda Urick, Medical College of Wisconsin.; SMAD5 antibody - middle region (ARP32006_P050) in r-SMAD5 cells using Western Blot
ARP32006_P050 - SMAD5
Human HepG2; WB Suggested Anti-SMAD5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; SMAD5 antibody - middle region (ARP32006_P050) in Human HepG2 cells using Western Blot
H00004090-M05 - SMAD5
SMAD5 monoclonal antibody (M05), clone 4F10 Western Blot analysis of SMAD5 expression in HeLa ( Cat # L013V1 ).
H00004090-M05 - SMAD5
Detection limit for recombinant GST tagged SMAD5 is approximately 0.1ng/ml as a capture antibody.
H00004090-M08 - SMAD5
SMAD5 monoclonal antibody (M08), clone 5E12 Western Blot analysis of SMAD5 expression in HeLa ( Cat # L013V1 ).
H00004090-M08 - SMAD5
Detection limit for recombinant GST tagged SMAD5 is approximately 0.1ng/ml as a capture antibody.
H00004090-M11 - SMAD5
SMAD5 monoclonal antibody (M11), clone 1C1 Western Blot analysis of SMAD5 expression in C32 ( Cat # L002V1 ).
H00004090-B01 - SMAD5
Western Blot analysis of SMAD5 expression in transfected 293T cell line (H00004090-T01) by SMAD5 MaxPab polyclonal antibody.Lane 1: SMAD5 transfected lysate(51.15 KDa).Lane 2: Non-transfected lysate.
AM06562SU-N - SMAD5
Immunohistochemical analysis of paraffin-embedded brain tissues (left) and lung cancer tissues (right) using SMAD5 antibody Cat.-No AM06562SU-N with DAB staining.
AM06562SU-N - SMAD5
Immunofluorescence analysis of NTERA-2 cells using SMAD5 antibody Cat.-No AM06562SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
AM06562SU-N - SMAD5
Western blot analysis using SMAD5 antibody Cat.-No AM06562SU-N against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.
AM06562SU-N - SMAD5
Flow cytometric analysis of Jurkat cells using SMAD5 antibody Cat.-No AM06562SU-N (green) and negative control (purple).
CPA1710-100ul - SMAD5
Western blot analysis of SMAD5 expression in HeLa (A); mouse testis (B); PC12 (C) whole cell lysates.
TP760815 - SMAD5
TA323512 - SMAD5
Predicted band size: 52 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds)
TA323512 - SMAD5
Predicted cell location: Cytoplasm. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using SMAD5 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200)
TA323513 - SMAD5
Predicted cell location: Cytoplasm. Positive control: Human breast and lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using SMAD5 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200)
TA323513 - SMAD5
Predicted cell location: Cytoplasm. Positive control: Human breast and lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using SMAD5 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x200)
TA329743 - SMAD5
WB Suggested Anti-SMAD5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate
TA329743 - SMAD5
Lanes: Lane 1: 5ug of transfected 293T lysate (SMAD1); Lane 1: 5ug of transfected 293T lysate (SMAD2); Lane 1: 5ug of transfected 293T lysate (SMAD3); Lane 1: 5ug of transfected 293T lysate (SMAD4); Lane 1: 5ug of transfected 293T lysate (SMAD5); Lane 1:
TA329744 - SMAD5
WB Suggested Anti-SMAD5 Antibody; Titration: 2.5 ug/ml; Positive Control: HepG2 Whole Cell
TA332472 - SMAD5
Western blot analysis of extracts of K562 cell lines, using SMAD5 antibody.
TA347048 - SMAD5
Western blot detection of SMAD5 (C-terminus) in Hela,Jurkat and K562 cell lysates using SMAD5 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:60KDa.
TA347048 - SMAD5
Immunocytochemistry of HeLa cells using anti-SMAD5 (C-terminus) mouse mAb diluted 1:75.
TA347048 - SMAD5
Flow Cytometry analysis of Jurkat cells stained with SMAD5 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.
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Catalog No. Host Iso. Clone Pres. React. Applications  

SMAD5 antibody
Immunohistochemical analysis of paraffin-embedded brain tissues (left) and lung cancer tissues (right) using SMAD5 antibody Cat.-No AM06562SU-N with DAB staining. Mouse IgG1 3H9 Ascites Hu, Rt E, F, ICC/IF, P, WB
0.1 ml / $380.00
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  1. Enzyme Immunoassay (E) (1)
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