SMAD5 antibody
Principal name
SMAD5 antibody
Alternative names for SMAD5 antibody
SMAD family member 5, SMAD 5, SMAD-5, MADH5, MAD homolog 5, JV5-1, Mothers against decapentaplegic homolog 5, Mothers against DPP homolog 5
Ncbi ID
NP_001001419.1, 9606, NP_001001420, NP_005894, NP_001157513.1, NM_001164042, NM_008541, NM_021692
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), ELISA (detection) (E(detection)), Flow Cytometry (F), Paraffin Sections (P)
Background of SMAD5 antibody
Bone morphogenetic proteins (BMPs) constitute a large family of sigling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determition, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-β family of Ser/Thr kise receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-termil motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5).MAP kises and CDKs 8 and 9 phosphorylate residues in the linker region of Smad1, including Ser206. The phosphorylation of Ser206 recruits Smurf1 to the linker region and leads to the degradation of Smad1 (6). Phosphorylation of this site also promotes Smad1 transcriptiol action by recruiting YAP to the linker region (7).
General readings
Langenfeld,E.M., et al., (2006) Oncogene 25 (5), 685-692
Immunofluorescence of monoclonal antibody to SMAD5 on HeLa cell. [antibody concentration 10 ug/ml]
SMAD5 monoclonal antibody (M01), clone 2D7 Western Blot analysis of SMAD5 expression in Hela NE ( Cat # L013V3 ).
SMAD5 monoclonal antibody (M01), clone 2D7. Western Blot analysis of SMAD5 expression in K-562 ( Cat # L009V1 ).
SMAD5 monoclonal antibody (M01), clone 2D7. Western Blot analysis of SMAD5 expression in Jurkat ( Cat # L017V1 ).
Detection limit for recombinant GST tagged SMAD5 is approximately 0.1ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to SMAD5 on HeLa cell. [antibody concentration 10 ug/ml]
SMAD5 monoclonal antibody (M03), clone 3A10 Western Blot analysis of SMAD5 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged SMAD5 is approximately 0.3ng/ml as a capture antibody.
Human HepG2; WB Suggested Anti-SMAD5 Antibody. . Titration: 2.5 ug/ml. . Positive Control: HepG2 Whole Cell; SMAD5 antibody - middle region (ARP32006_T100) in Human HepG2 cells using Western Blot
r-SMAD5; . Lanes: Lane 1: 5ug of transfected 293T lysate (SMAD1) . Lane 1: 5ug of transfected 293T lysate (SMAD2) . Lane 1: 5ug of transfected 293T lysate (SMAD3) . Lane 1: 5ug of transfected 293T lysate (SMAD4) . Lane 1: 5ug of transfected 293T lysate (SMAD5) . Lane 1: 5ug of transfected 293T lysate (SMAD6) . Lane 1: 5ug of transfected 293T lysate (SMAD7) . Lane 1: 5ug of transfected 293T lysate (SMAD8) . Lane 1: 5ug of transfected 293T lysate (GFP). Primary Antibody Dilution: 1:1000. Secondary Antibody: Goat anti-Rabbit IgG HRP Conjugated. Secondary Antibody Dilution: 1:10,000. Gene Name: SMAD5. Submitted by: Amanda Urick, Medical College of Wisconsin.; SMAD5 antibody - middle region (ARP32006_P050) in r-SMAD5 cells using Western Blot
Human HepG2; WB Suggested Anti-SMAD5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; SMAD5 antibody - middle region (ARP32006_P050) in Human HepG2 cells using Western Blot
SMAD5 monoclonal antibody (M05), clone 4F10 Western Blot analysis of SMAD5 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged SMAD5 is approximately 0.1ng/ml as a capture antibody.
SMAD5 monoclonal antibody (M08), clone 5E12 Western Blot analysis of SMAD5 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged SMAD5 is approximately 0.1ng/ml as a capture antibody.
SMAD5 monoclonal antibody (M11), clone 1C1 Western Blot analysis of SMAD5 expression in C32 ( Cat # L002V1 ).
Western Blot analysis of SMAD5 expression in transfected 293T cell line (H00004090-T01) by SMAD5 MaxPab polyclonal antibody.Lane 1: SMAD5 transfected lysate(51.15 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical analysis of paraffin-embedded brain tissues (left) and lung cancer tissues (right) using SMAD5 antibody Cat.-No AM06562SU-N with DAB staining.
Immunofluorescence analysis of NTERA-2 cells using SMAD5 antibody Cat.-No AM06562SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot analysis using SMAD5 antibody Cat.-No AM06562SU-N against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.
Flow cytometric analysis of Jurkat cells using SMAD5 antibody Cat.-No AM06562SU-N (green) and negative control (purple).
Western blot analysis of SMAD5 expression in HeLa (A); mouse testis (B); PC12 (C) whole cell lysates.
Predicted band size: 52 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds)
Predicted cell location: Cytoplasm. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using SMAD5 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200)
Predicted cell location: Cytoplasm. Positive control: Human breast and lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using SMAD5 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human breast and lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using SMAD5 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x200)
WB Suggested Anti-SMAD5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate
Lanes: Lane 1: 5ug of transfected 293T lysate (SMAD1); Lane 1: 5ug of transfected 293T lysate (SMAD2); Lane 1: 5ug of transfected 293T lysate (SMAD3); Lane 1: 5ug of transfected 293T lysate (SMAD4); Lane 1: 5ug of transfected 293T lysate (SMAD5); Lane 1:
WB Suggested Anti-SMAD5 Antibody; Titration: 2.5 ug/ml; Positive Control: HepG2 Whole Cell
Western blot analysis of extracts of K562 cell lines, using SMAD5 antibody.
Western blot detection of SMAD5 (C-terminus) in Hela,Jurkat and K562 cell lysates using SMAD5 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:60KDa.
Immunocytochemistry of HeLa cells using anti-SMAD5 (C-terminus) mouse mAb diluted 1:75.
Flow Cytometry analysis of Jurkat cells stained with SMAD5 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA323512 | SMAD5 antibody |
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| Anti-SMAD5 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
$335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA323513 | SMAD5 antibody |
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| Anti-SMAD5 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
$335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA332472 | SMAD5 antibody |
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| Rabbit anti-SMAD5 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
$335.00
|
||
| OriGene Technologies, Inc. | |||||||
| AP00199PU-N | SMAD5 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
$380.00
|
||
| OriGene Technologies GmbH | |||||||