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SIX3 antibody

Principal name

SIX3 antibody

Alternative names for SIX3 antibody

Homeobox protein SIX3, Sine oculis homeobox homolog 3

SwissProt ID

O42406 (Chick), O95343 (Human), Q62233 (Mouse)

Gene ID

6496 (SIX3), 20473 (Six3)

Ncbi ID

9606, NP_005404, NM_011381, NP_005404.1, NM_005413

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), GP (Guinea Pig)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of SIX3 antibody

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Two of these family members Six3 and Six6 (also designated Optx2 and Six9) are required for the specification and proliferation of the eye field in vertebrates, and, therefore, are the vertebrate homologues most closely related to the Drosophila sine oculis protein, which has an essential role in controlling compound eye development. Six3 and Six6 expression largely overlap during development of specific tissues, such as retina, hypothalamus, and pituitary. The human Six6 gene maps to chromosome 14q22.3-q23. Haploinsufficiency of Six6 may cause several developmental disorders, including bilateral anophthalmia and pituitary anomalies. The gene encoding the human Six3 protein maps to chromosome 2p21-p22, a region associated with holoprosencephaly type 2 (HPE2). Deletion of Six3 may be associated with HPE2 disorder, a common, severe malformation of the brain that results from incomplete cleavage of the forebrain during early embryogenesis.

General readings

Appolloni,I., (2008) Cereb. Cortex 18 (3), 553-562

SIX3 monoclonal antibody (M01), clone 3D12. Western Blot analysis of SIX3 expression in human thyroid(diffuse hyperplasia).

H00006496-M01

SIX3 monoclonal antibody (M01), clone 3D12. Western Blot analysis of SIX3 expression in MES-SA/Dx5 ( Cat # L021V1 ).

H00006496-M01

SIX3 monoclonal antibody (M01), clone 3D12 Western Blot analysis of SIX3 expression in 293 ( Cat # L026V1 ).

H00006496-M01

SIX3 monoclonal antibody (M02), clone 3F1. Western Blot analysis of SIX3 expression in human thyroid(diffuse hyperplasia).

H00006496-M02

SIX3 monoclonal antibody (M02), clone 3F1. Western Blot analysis of SIX3 expression in FHs 173 WE.

H00006496-M02

SIX3 monoclonal antibody (M02), clone 3F1. Western Blot analysis of SIX3 expression in MES-SA/Dx5 ( Cat # L021V1 ).

H00006496-M02

SIX3 monoclonal antibody (M02), clone 3F1 Western Blot analysis of SIX3 expression in 293 ( Cat # L026V1 ).

H00006496-M02

SIX3 monoclonal antibody (M04), clone 1A2 Western Blot analysis of SIX3 expression in 293 ( Cat # L026V1 ).

H00006496-M04

SIX3 monoclonal antibody (M04), clone 1A2. Western Blot analysis of SIX3 expression in human thyroid(diffuse hyperplasia).

H00006496-M04

SIX3 monoclonal antibody (M04), clone 1A2. Western Blot analysis of SIX3 expression in FHs 173 WE.

H00006496-M04

SIX3 monoclonal antibody (M04), clone 1A2. Western Blot analysis of SIX3 expression in MES-SA/Dx5 ( Cat # L021V1 ).

H00006496-M04

SIX3 monoclonal antibody (M08), clone 2B9. Western Blot analysis of SIX3 expression in human thyroid(diffuse hyperplasia).

H00006496-M08

SIX3 monoclonal antibody (M08), clone 2B9. Western Blot analysis of SIX3 expression in FHs 173 WE.

H00006496-M08

SIX3 monoclonal antibody (M08), clone 2B9. Western Blot analysis of SIX3 expression in MES-SA/Dx5 ( Cat # L021V1 ).

H00006496-M08

SIX3 monoclonal antibody (M08), clone 2B9 Western Blot analysis of SIX3 expression in 293 ( Cat # L026V1 ).

H00006496-M08

SIX3 monoclonal antibody (M10), clone 4H6. Western Blot analysis of SIX3 expression in human thyroid(diffuse hyperplasia).

H00006496-M10

SIX3 monoclonal antibody (M10), clone 4H6. Western Blot analysis of SIX3 expression in FHs 173 WE.

H00006496-M10

SIX3 monoclonal antibody (M10), clone 4H6. Western Blot analysis of SIX3 expression in MES-SA/Dx5 ( Cat # L021V1 ).

H00006496-M10

SIX3 monoclonal antibody (M10), clone 4H6 Western Blot analysis of SIX3 expression in 293 ( Cat # L026V1 ).

H00006496-M10

Western blot (WB) analysis of Six3/6 antibody Cat.-No AP06651PU-N in extracts from HUVEC cells.

AP06651PU-N

Western blot (WB) analysis of Six3/6 antibody Cat.-No AP06651PU-N at 1/500 dilution.
Lane 1: HEK293T whole cell lysate.
Lane 2: Raw264.7 whole cell lysate.
Lane 3: H9C2 whole cell lysate.

AP06651PU-N

Human HepG2; WB Suggested Anti-SIX3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; SIX3 antibody - N-terminal region (ARP32369_P050) in Human HepG2 cells using Western Blot

ARP32369_P050

Mouse Thymus; WB Suggested Anti-Six3 Antibody Titration: 0.2-1 ug/ml. Positive Control: Mouse Thymus; Six3 antibody - C-terminal region (ARP37135_P050) in Mouse Thymus cells using Western Blot

ARP37135_P050

Western blot analysis of SIX3 expression in HeLa (A); Jurkat (B); Raw264.7 (C); PC12 (D) whole cell lysates.

Western blot analysis of extracts from A549 cells, using SIX3 antibody.The lane on the right is treated with the synthesized peptide.

TA314537

SIX3 Antibody (Center) (Cat. #TA324591) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the SIX3 antibody detected the SIX3 protein (arrow).