SH2D1A antibody
Principal name
SH2D1A antibody
Alternative names for SH2D1A antibody
DSHP, SAP, SH2 domain-containing protein 1A, SLAM-associated protein, T-cell signal transduction molecule SAP, Duncan disease SH2-protein, Signaling lymphocytic activation molecule-associated protein
Ncbi ID
9606, NP_001108409, NM_002351, NP_001108409.1, NP_002342, NP_035494.1, NM_011364, NM_001109313, NM_001114937
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP)
Background of SH2D1A antibody
- Kit Component:
- - KN315699G1, Sh2d1a gRNA vector 1 in pCas-Guide vector
- KN315699G2, Sh2d1a gRNA vector 2 in pCas-Guide vector
- KN315699D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Romero X, Benitez D, March S, Vilella R, Miralpeix M, Engel P. Differential expression of SAP and EAT-2-binding leukocyte cell-surface molecules CD84, CD150 (SLAM), CD229 (Ly9) and CD244 (2B4). Tissue Antigens. 2004 Aug;64(2):132-44.
Western Blot analysis of SH2D1A expression in transfected 293T cell line by SH2D1A monoclonal antibody (M01), clone 1C9.Lane 1: SH2D1A transfected lysate(14.2 KDa).Lane 2: Non-transfected lysate.
SH2D1A monoclonal antibody (M01), clone 1C9 Western Blot analysis of SH2D1A expression in Jurkat ( Cat # L017V1 ).
Western Blot analysis of SH2D1A expression in transfected 293T cell line (H00004068-T01) by SH2D1A MaxPab polyclonal antibody.Lane 1: SH2D1A transfected lysate(14.08 KDa).Lane 2: Non-transfected lysate.
Western blot analysis using SAP antibody on NK-92 cell lysate at 10 µg/ml).
SH2D1A polyclonal antibody ( Cat # PAB6819 ) staining ( 0.3 µg/ml ) of human thymus lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
HEK293 overexpressing SH2D1A and probed with SH2D1A polyclonal antibody ( Cat # PAB6819 ) ( mock transfection in first lane ), tested by Origene.
SH2D1A polyclonal antibody ( Cat # PAB6907 ) ( 0.2 µg/ml ) staining of human lymph node lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot analysis of SH2D1A expression in transfected 293T cell line (H00004068-T01) by SH2D1A MaxPab polyclonal antibody.Lane 1: SH2D1A transfected lysate(14.20 KDa).Lane 2: Non-transfected lysate.
SH2D1A MaxPab rabbit polyclonal antibody. Western Blot analysis of SH2D1A expression in Jurkat.
Western Blot analysis of SH2D1A expression in transfected 293T cell line (H00004068-T02) by SH2D1A MaxPab polyclonal antibody.Lane 1: SH2D1A transfected lysate(14.08 KDa).Lane 2: Non-transfected lysate.
Western blot analysis using SH2D1A polyclonal antibody ( Cat # PAB10367 ) on NK-92 cell lysate at 10µg/ml).
Recombinant human SH2D1A, 1-128aa, His-tagged
Human Jurkat; WB Suggested Anti-SH2D1A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. SH2D1A is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; SH2D1A antibody - C-terminal region (ARP60359_P050) in Human Jurkat cells using Western Blot
Western blot analysis of SH2D1A expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates.
TA302605 staining (0.3µg/ml) of Human Thymus lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA302606 (0.2µg/ml) staining of human lymph node lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of thymus cell lysate using SH2D1A antibody.
Immunohistochemistry of paraffin-embedded human stomach cancer using SH2D1A antibody at dilution of 1:200 (400x lens)
Immunohistochemistry of paraffin-embedded rat lung using SH2D1A antibody at dilution of 1:200 (400x lens).
Immunofluorescence analysis of HeLa cell using SH2D1A antibody. Blue: DAPI for nuclear staining.
WB Suggested Anti-SH2D1A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate SH2D1A is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
Host: Rabbit; Target Name: SH2D1A; Sample Tissue: 293T Whole Cell lysates; Antibody Dilution: 1.0 ug/ml
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304723 | SH2D1A (transcript variant 1) |
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| Recombinant protein of human SH2 domain protein 1A (SH2D1A), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
$748.00
|
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| OriGene Technologies, Inc. | |||||