RUNX1T1 antibody
Principal name
RUNX1T1 antibody
Alternative names for RUNX1T1 antibody
AML1T1, CBFA2T1, MTG8, ZMYND2, Cyclin-D-related protein, CDR, Eight twenty one protein, ETO, Zinc finger MYND domain-containing protein 2
Ncbi ID
9606, NP_783554, NP_004340, NP_001185554.1, NM_175634, NP_783553, NP_033952.1
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of RUNX1T1 antibody
RUNX1T1 is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Several transcript variants encoding multiple isoforms have been found for this gene.
General readings
Lausen,J., et al., (2004) J. Biol. Chem. 279 (47), 49281-49288
Western Blot analysis of RUNX1T1 expression in transfected 293T cell line by RUNX1T1 monoclonal antibody (M01), clone 5A12.Lane 1: RUNX1T1 transfected lysate(67.566 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged RUNX1T1 is approximately 0.3ng/ml as a capture antibody.
Human 721_B; Host: Rabbit. Target Name: WT1 . Sample Tissue: 721_B . Antibody Dilution: 1.0ug/ml. RUNX1T1 is supported by BioGPS gene expression data to be expressed in 721_B.; RUNX1T1 antibody - middle region (ARP33996_T100) in Human 721_B cells using Western Blot
Human HepG2; WB Suggested Anti-RUNX1T1 Antibody Titration: 1.25ug/ml. Positive Control: HepG2 cell lysate. There is BioGPS gene expression data showing that RUNX1T1 is expressed in HepG2.; RUNX1T1 antibody - middle region (ARP33996_T100) in Human HepG2 cells using Western Blot
Western Blot analysis of RUNX1T1 expression in transfected 293T cell line (H00000862-T01) by RUNX1T1 MaxPab polyclonal antibody.Lane 1: RUNX1T1 transfected lysate(63.47 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Analysis (PLA) of protein-protein interactions between RUNX1T1 and HDAC1 HeLa cells were stained with anti-RUNX1T1 rabbit purified polyclonal 1:1200 and anti-HDAC1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Western Blot analysis of RUNX1T1 expression in transfected 293T cell line (H00000862-T01) by RUNX1T1 MaxPab polyclonal antibody.Lane 1: RUNX1T1 transfected lysate(64.40 KDa).Lane 2: Non-transfected lysate.
Western blot was performed on seven GST-fusion proteins containing different fragments of RUNX1T1 ( Lane 1~7 ).RUNX1T1 polyclonal antibody ( Cat # PAB14127 ) was used at dilution 1 : 1,000 in TBS-Tween containing 5% skimmed milk.
Western blot was performed on nuclear extracts from KAS-6/1 cells ( human myeloma cell line, lane 1 ) and SKNO-1 cells ( human acute myeloblastic leukaemia, lane 2 ) with RUNX1T1 polyclonal antibody ( Cat # PAB14127 ), diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.The location of RUNX1T1 and a presumed splice variant ( missing 106 C-terminal amino acids ) are indicated on the right.
ELISA was performed using a serial dilution of RUNX1T1 polyclonal antibody ( Cat # PAB14127 ), crude serum and flow through in antigen coated wells.The synthetic peptide used to immunise the rabbits, served as antigen.By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1 : 5,250.
Human 293T; WB Suggested Anti-RUNX1T1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate; RUNX1T1 antibody - middle region (ARP58127_P050) in Human 293T cells using Western Blot
Anti-RUNX1T1 mouse monoclonal antibody (TA504703) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RUNX1T1(RC222426).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUNX1T1 (RC222426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RUNX1T1.
Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-RUNX1T1 monoclonal antibody at 1:200 dilution.
ELISA: RUNX1T1 / ETO Antibody
1Human 721_B; Host: Rabbit; Target Name: WT1; Sample Tissue: 721_B; Antibody Dilution: 1.0 ug/mlRUNX1T1 is supported by BioGPS gene expression data to be expressed in 721_B
WB Suggested Anti-RUNX1T1 Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that RUNX1T1 is expressed in HepG2
WB Suggested Anti-RUNX1T1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysate
ChIP assays were performed using SKNO-1 cells, the antibody against ETO and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Image shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used.
The IP’d DNA of 6 ChIP’s was pooled and analysed with an Illumina Genome Analyzer. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Image shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.
Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against human ETO. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,300.
WB was performed on seven GST-fusion proteins containing different fragments of AML-ETO (1-7) with the ab at 1:1,000. The antibody raised against AML-ETO recognizes the ETO part (lane 2) of the fusion protein. C: WB was performed on nuclear extracts from KAS-6/1 cells (human myeloma cell line) and SKNO-1 cells (human acute myeloblastic leukaemia) with the ab against AML-ETO at 1:1,000 . The location of AML-ETO and a presumed splice variant (missing 106 C-terminal amino acids) are indicated.
Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of antibody against AML-ETO, crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,250.
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (RUNX1T1 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA351613 | RUNX1T1 antibody |
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| Rabbit Polyclonal Anti-RUNX1T1 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
$335.00
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| OriGene Technologies, Inc. | |||||||